AB17958

Anti-SMC4 antibody

Name: Anti-SMC4 antibody (ab17958) | Abcam
Brand: Abcam Limited
SKU: AB17958

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(14 Publications)

Rabbit Polyclonal SMC4 antibody. Suitable for WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human SMC4.

View Alternative Names

CAPC, SMC4L1, SMC4, Structural maintenance of chromosomes protein 4, SMC protein 4, SMC-4, Chromosome-associated polypeptide C, XCAP-C homolog, hCAP-C

1 Images

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Western blot - Anti-SMC4 antibody (AB17958)

ab17958 at 0.2μg/ml detecting SMC4 from 90μg of whole cell lysate from 293T cells (left hand lane) or from normal human lymphoblasts (right hand lane) by western blot. The blot was detected by ECL. ab17958 at 0.2µg/ml detecting SMC4 from 90µg of whole cell lysate from 293T cells (left hand lane) or from normal human lymphoblasts (right hand lane) by western blot. The blot was detected by ECL.

All lanes:

Western blot - Anti-SMC4 antibody (ab17958)

Predicted band size: 147 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human SMC4. The exact immunogen used to generate this antibody is proprietary information.

Q9NTJ3

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC4 also known as Structural Maintenance of Chromosomes protein 4 is a core component of the condensin complex. It weighs approximately 141 kDa and localizes to the nucleus of cells. SMC4 plays a mechanical role during mitosis where it ensures chromosomes condense correctly allowing for proper segregation. This protein's expression occurs widely across different tissue types reflecting its critical involvement in cell division and genomic integrity.

Biological function summary

SMC4 operates as a part of the condensin complex which consists of several proteins including SMC2. This complex is essential in chromosome architecture mediating the compression and cohesion of sister chromatids. This structural role stabilizes chromosomes to prevent damage during cell cycle phases. By maintaining chromosomal morphology SMC4 contributes to genetic stability across generations of cells.

Pathways

SMC4 functions prominently within the chromosome segregation and cell cycle pathways. Alongside its partner SMC2 it helps manage the chromosomal landscape during mitotic events. This positioning within pathways highlights interactions with other key proteins such as cohesin which also plays a role in chromosome cohesion. By ensuring proper chromosomal condensation SMC4 synchronizes with various checkpoints throughout the cell cycle.

SMC4 has links to certain cancers including glioblastoma and colorectal cancer where abnormal expression or function can disrupt mitosis and promote genomic instability. In these contexts SMC4 often interacts with other cancer-related proteins such as p53 which plays roles in cell cycle regulation and apoptosis. The misregulation of SMC4 can contribute to unchecked cell proliferation and tumor development making it a significant focus of cancer research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

See full target information SMC4

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 239:e31445 PubMed39344989

2024

The role of TREM-1 in septic myocardial pyroptosis and septic cardiomyopathy in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxia Chen,Lixia Mao,Songtao Liu,Shunyi Huang,Qiuyun Lin,Man Zeng,Huiyi Huang,Xiaocong Sun,Hongpeng Chen,Jiahao Huang,Gaosheng Zhou,Liehua Deng

Nature communications 13:1474 PubMed35304442

2022

A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats.

Applications

Unspecified application

Species

Unspecified reactive species

Shelby Winans,Hyun Jae Yu,Kenia de Los Santos,Gary Z Wang,Vineet N KewalRamani,Stephen P Goff

Genome biology 23:70 PubMed35241136

2022

Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Eui-Hwan Choi,Seobin Yoon,Young Eun Koh,Tae Kyung Hong,Jeong Tae Do,Bum-Kyu Lee,Yoonsoo Hahn,Keun P Kim

Frontiers in oncology 11:761693 PubMed34868977

2021

The Clinical Significance and Transcription Regulation of a DNA Damage Repair Gene, SMC4, in Low-Grade Glioma Integrated Bioinformatic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Zhisheng Wu

Journal of cellular physiology 236:3579-3598 PubMed33078399

2020

Cancer-associated mutations in the condensin II subunit CAPH2 cause genomic instability through telomere dysfunction and anaphase chromosome bridges.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Weyburne,Giovanni Bosco

Nature cell biology 21:1393-1402 PubMed31685986

2019

A chromosome folding intermediate at the condensin-to-cohesin transition during telophase.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Abramo,Anne-Laure Valton,Sergey V Venev,Hakan Ozadam,A Nicole Fox,Job Dekker

International journal of cell biology 2017:8607532 PubMed29445399

2017

Nesprin-2 Interacts with Condensin Component SMC2.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xing,Carmen Mroß,Linlin Hao,Martina Munck,Alexandra Herzog,Clara Mohr,C P Unnikannan,Pranav Kelkar,Angelika A Noegel,Ludwig Eichinger,Sascha Neumann

Genes & development 29:1683-95 PubMed26302788

2015

A structural hierarchy mediated by multiple nuclear factors establishes IgH locus conformation.

Applications

ChIP

Species

Mouse

Tatiana Gerasimova,Changying Guo,Amalendu Ghosh,Xiang Qiu,Lindsey Montefiori,Jiyoti Verma-Gaur,Nancy M Choi,Ann J Feeney,Ranjan Sen

Cell cycle (Georgetown, Tex.) 14:2160-70 PubMed26017022

2015

A novel role for the condensin II complex in cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhki Yokoyama,Hengrui Zhu,Rugang Zhang,Ken-ichi Noma

Cell cycle (Georgetown, Tex.) 13:1115-31 PubMed24553121

2014

Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuko Murakami-Tonami,Satoshi Kishida,Ichiro Takeuchi,Yuki Katou,John M Maris,Hitoshi Ichikawa,Yutaka Kondo,Yoshitaka Sekido,Katsuhiko Shirahige,Hiroshi Murakami,Kenji Kadomatsu

View all publications

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Product promise

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Anti-SMC4 antibody

Western blot - Anti-SMC4 antibody (AB17958)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Complementary Products

Secondary Antibodies

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Primary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise