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AB250130

Anti-SMC4 antibody [EPR13366] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SMC4 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CAPC, SMC4L1, SMC4, Structural maintenance of chromosomes protein 4, SMC protein 4, SMC-4, Chromosome-associated polypeptide C, XCAP-C homolog, hCAP-C

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - BSA and Azide free (AB250130)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - BSA and Azide free (AB250130)

This data was developed using ab179831, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells labeling SMC4 with ab179831 at 1/50 dilution.

Western blot - Anti-SMC4 antibody [EPR13366] - BSA and Azide free (AB250130)
  • WB

Supplier Data

Western blot - Anti-SMC4 antibody [EPR13366] - BSA and Azide free (AB250130)

This data was developed using ab179831, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (<a href='/en-us/products/primary-antibodies/smc4-antibody-epr13366-c-terminal-ab179831'>ab179831</a>) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 147 kDa

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Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - BSA and Azide free (AB250130)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - BSA and Azide free (AB250130)

This data was developed using ab179831, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Raji cells using ab179831 at 1/10 (red) or a rabbit IgG negative (green).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13366

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250130 is the carrier-free version of ab179831.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC4 also known as Structural Maintenance of Chromosomes protein 4 is a core component of the condensin complex. It weighs approximately 141 kDa and localizes to the nucleus of cells. SMC4 plays a mechanical role during mitosis where it ensures chromosomes condense correctly allowing for proper segregation. This protein's expression occurs widely across different tissue types reflecting its critical involvement in cell division and genomic integrity.
Biological function summary

SMC4 operates as a part of the condensin complex which consists of several proteins including SMC2. This complex is essential in chromosome architecture mediating the compression and cohesion of sister chromatids. This structural role stabilizes chromosomes to prevent damage during cell cycle phases. By maintaining chromosomal morphology SMC4 contributes to genetic stability across generations of cells.

Pathways

SMC4 functions prominently within the chromosome segregation and cell cycle pathways. Alongside its partner SMC2 it helps manage the chromosomal landscape during mitotic events. This positioning within pathways highlights interactions with other key proteins such as cohesin which also plays a role in chromosome cohesion. By ensuring proper chromosomal condensation SMC4 synchronizes with various checkpoints throughout the cell cycle.

SMC4 has links to certain cancers including glioblastoma and colorectal cancer where abnormal expression or function can disrupt mitosis and promote genomic instability. In these contexts SMC4 often interacts with other cancer-related proteins such as p53 which plays roles in cell cycle regulation and apoptosis. The misregulation of SMC4 can contribute to unchecked cell proliferation and tumor development making it a significant focus of cancer research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
See full target information SMC4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:12868-12884 PubMed32580945

2020

Adipose mesenchymal stem cell-derived extracellular vesicles containing microRNA-26a-5p target TLR4 and protect against diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yurui Duan,Qingyang Luo,Yun Wang,Yali Ma,Fang Chen,Xiaoguang Zhu,Jun Shi
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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