AB229213

Anti-SMC4 antibody - N-terminal

Name: Anti-SMC4 antibody - N-terminal (ab229213) | Abcam
Brand: Abcam Limited
SKU: AB229213
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal SMC4 antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMC4.

View Alternative Names

CAPC, SMC4L1, SMC4, Structural maintenance of chromosomes protein 4, SMC protein 4, SMC-4, Chromosome-associated polypeptide C, XCAP-C homolog, hCAP-C

2 Images

Supplier Data

Immunoprecipitation - Anti-SMC4 antibody - N-terminal (AB229213)

SMC4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg of ab229213. Western blot was performed from the immunoprecipitate using ab229213. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : HEK-293T whole cell extract (Input).

Lane 2 : Control IgG instead of ab229213 in HEK-293T whole cell extract.

Lane 3 : ab229213 IP in HEK-293T whole cell extract.

All lanes:

Immunoprecipitation - Anti-SMC4 antibody - N-terminal (ab229213)

Predicted band size: 147 kDa

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Supplier Data

Western blot - Anti-SMC4 antibody - N-terminal (AB229213)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-SMC4 antibody - N-terminal (ab229213) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 147 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human SMC4. The exact immunogen used to generate this antibody is proprietary information.

Q9NTJ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC4 also known as Structural Maintenance of Chromosomes protein 4 is a core component of the condensin complex. It weighs approximately 141 kDa and localizes to the nucleus of cells. SMC4 plays a mechanical role during mitosis where it ensures chromosomes condense correctly allowing for proper segregation. This protein's expression occurs widely across different tissue types reflecting its critical involvement in cell division and genomic integrity.

Biological function summary

SMC4 operates as a part of the condensin complex which consists of several proteins including SMC2. This complex is essential in chromosome architecture mediating the compression and cohesion of sister chromatids. This structural role stabilizes chromosomes to prevent damage during cell cycle phases. By maintaining chromosomal morphology SMC4 contributes to genetic stability across generations of cells.

Pathways

SMC4 functions prominently within the chromosome segregation and cell cycle pathways. Alongside its partner SMC2 it helps manage the chromosomal landscape during mitotic events. This positioning within pathways highlights interactions with other key proteins such as cohesin which also plays a role in chromosome cohesion. By ensuring proper chromosomal condensation SMC4 synchronizes with various checkpoints throughout the cell cycle.

SMC4 has links to certain cancers including glioblastoma and colorectal cancer where abnormal expression or function can disrupt mitosis and promote genomic instability. In these contexts SMC4 often interacts with other cancer-related proteins such as p53 which plays roles in cell cycle regulation and apoptosis. The misregulation of SMC4 can contribute to unchecked cell proliferation and tumor development making it a significant focus of cancer research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

See full target information SMC4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:30681 PubMed39730417

2024

Chronic unpredictable stress induces anxiety-like behavior and oxidative stress, leading to diminished ovarian reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihao Zhou,Yangshuo Li,Jie Ding,Shuai Sun,Wen Cheng,Jin Yu,Zailong Cai,Zhexin Ni,Chaoqin Yu

The EMBO journal 42:e113475 PubMed37357575

2023

Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Batty,Christoph Ch Langer,Zsuzsanna Takács,Wen Tang,Claudia Blaukopf,Jan-Michael Peters,Daniel W Gerlich

Nature 609:183-190 PubMed35922507

2022

A mitotic chromatin phase transition prevents perforation by microtubules.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian W G Schneider,Bryan A Gibson,Shotaro Otsuka,Maximilian F D Spicer,Mina Petrovic,Claudia Blaukopf,Christoph C H Langer,Paul Batty,Thejaswi Nagaraju,Lynda K Doolittle,Michael K Rosen,Daniel W Gerlich

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMC4 antibody - N-terminal

Immunoprecipitation - Anti-SMC4 antibody - N-terminal (AB229213)

Western blot - Anti-SMC4 antibody - N-terminal (AB229213)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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