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Rabbit Polyclonal SMC5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Structural maintenance of chromosomes protein 5 aa 250-500.

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Images

Western blot - Anti-SMC5 antibody (AB154103), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Structural maintenance of chromosomes protein 5 aa 250-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IY18

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541).

Alternative names

Recommended products

Rabbit Polyclonal SMC5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Structural maintenance of chromosomes protein 5 aa 250-500.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Structural maintenance of chromosomes protein 5 aa 250-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IY18
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SMC5 also known as Structural Maintenance of Chromosomes 5 is a protein that plays a significant role in chromosome segregation and stability. It has a molecular mass of approximately 122 kDa. This protein is widely expressed in many tissues with high levels observed in the brain liver and lung. SMC5 is a part of the SMC protein family and functions in connection with SMC6 forming the SMC5/6 complex. This complex is necessary for DNA repair and recombination processes.

Biological function summary

SMC5 influences the maintenance of genomic integrity during cell division. It is an essential component of the SMC5/6 complex which also includes non-SMC proteins like NSMCE1-4. The complex facilitates proper segregation of chromosomes by stabilizing replication forks and resolving DNA interlinkages. This mechanism is key in preventing genomic instability which can lead to diverse cellular dysfunctions.

Pathways

SMC5 is involved in the DNA damage response and homologous recombination repair pathways. It interacts with proteins like RAD51 which plays a role in the repair of double-strand breaks through homologous recombination. These pathways ensure that damaged DNA is accurately repaired preventing erroneous sequences from propagating during subsequent cell divisions.

Associated diseases and disorders

SMC5 has been linked to conditions such as cancer and Fanconi anemia. Mutations affecting the SMC5/6 complex can lead to defective DNA repair and accumulated genomic instability often observed in cancers. The complex's disruption also relates to Fanconi anemia a disorder that affects bone marrow function. There are associations with proteins like BRCA1 and FANCI both of which participate in the cellular response to DNA damage and repair highlighting the complex's medical relevance.

Product promise

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1 product image

  • Western blot - Anti-SMC5 antibody (ab154103), expandable thumbnail

    Western blot - Anti-SMC5 antibody (ab154103)

    5% SDS PAGE

    All lanes: Western blot - Anti-SMC5 antibody (ab154103) at 1/500 dilution

    All lanes: NT2D1 whole cell lysate at 30 µg

    Predicted band size: 129 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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