AB18038

Anti-SMC5 antibody

Name: Anti-SMC5 antibody (ab18038) | Abcam
Brand: Abcam Limited
SKU: AB18038
Price: 535 USD
Availability: InStock
Rating: 1 (2 reviews)

(2 Reviews)

(5 Publications)

Rabbit Polyclonal SMC5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SMC5 aa 1050 to C-terminus.

View Alternative Names

KIAA0594, SMC5L1, SMC5, Structural maintenance of chromosomes protein 5, SMC protein 5, SMC-5, hSMC5

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Supplier Data

Immunoprecipitation - Anti-SMC5 antibody (AB18038)

Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.

Lane 1 : ab18038 used for IP at 6 μg per reaction

Lane 2 : Control IgG

For blotting immunoprecipitated SMC5, ab18038 was used at 0.1 μg/ml. Detection : Chemiluminescence with an exposure time of 75 seconds.

All lanes:

Immunoprecipitation - Anti-SMC5 antibody (ab18038)

Predicted band size: 129 kDa

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Western blot - Anti-SMC5 antibody (AB18038)

ab18038 staining Human SMC5 at a concentration of 0.1 μg/ml by Western Blot, and 5 μg/0.5 mg lysate by Immunoprecipitation.

Samples :

A) Nuclear extract (10 and 20 μg) from HeLa cells.

B) Whole cell lysate (500 μg) from HeLa and 293T cells.

Detection : Chemiluminescence with exposure times of less than 2 minutes.

ab18038 staining Human SMC5 at a concentration of 0.1 μg/ml by Western Blot, and 5 μg/0.5 mg lysate by Immunoprecipitation.

Samples :
A) Nuclear extract (10 and 20 μg) from HeLa cells.
B) Whole cell lysate (500 μg) from HeLa and 293T cells.

Detection : Chemiluminescence with exposure times of less than 2 minutes.

All lanes:

Western blot - Anti-SMC5 antibody (ab18038)

Predicted band size: 129 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SMC5 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IY18

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/25000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was purified using an epitope specific to SMC5 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC5 also known as Structural Maintenance of Chromosomes 5 is a protein that plays a significant role in chromosome segregation and stability. It has a molecular mass of approximately 122 kDa. This protein is widely expressed in many tissues with high levels observed in the brain liver and lung. SMC5 is a part of the SMC protein family and functions in connection with SMC6 forming the SMC5/6 complex. This complex is necessary for DNA repair and recombination processes.

Biological function summary

SMC5 influences the maintenance of genomic integrity during cell division. It is an essential component of the SMC5/6 complex which also includes non-SMC proteins like NSMCE1-4. The complex facilitates proper segregation of chromosomes by stabilizing replication forks and resolving DNA interlinkages. This mechanism is key in preventing genomic instability which can lead to diverse cellular dysfunctions.

Pathways

SMC5 is involved in the DNA damage response and homologous recombination repair pathways. It interacts with proteins like RAD51 which plays a role in the repair of double-strand breaks through homologous recombination. These pathways ensure that damaged DNA is accurately repaired preventing erroneous sequences from propagating during subsequent cell divisions.

SMC5 has been linked to conditions such as cancer and Fanconi anemia. Mutations affecting the SMC5/6 complex can lead to defective DNA repair and accumulated genomic instability often observed in cancers. The complex's disruption also relates to Fanconi anemia a disorder that affects bone marrow function. There are associations with proteins like BRCA1 and FANCI both of which participate in the cellular response to DNA damage and repair highlighting the complex's medical relevance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed : 26983541).

See full target information SMC5

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 85:660-674 PubMed39652575

2024

HBV Remodels PP2A Complexes to Rewire Kinase Signaling in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rigney E Turnham,Adriana Pitea,Gwendolyn M Jang,Zhong Xu,Huat Chye Lim,Alex L Choi,John Von Dollen,Rebecca S Levin,James T Webber,Elizabeth McCarthy,Junjie Hu,Xiaolei Li,Li Che,Ananya Singh,Alex Yoon,Gary K L Chan,Robin K Kelley,Danielle L Swaney,Wei Zhang,Sourav Bandyopadhyay,Fabian J Theis,Manon Eckhardt,Xin Chen,Kevan M Shokat,Trey Ideker,Nevan J Krogan,John D Gordan

Cellular and molecular gastroenterology and hepatology 7:297-312 PubMed30704981

2019

Inhibition of HBV Transcription From cccDNA With Nitazoxanide by Targeting the HBx-DDB1 Interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuma Sekiba,Motoyuki Otsuka,Motoko Ohno,Mari Yamagami,Takahiro Kishikawa,Tatsunori Suzuki,Rei Ishibashi,Takahiro Seimiya,Eri Tanaka,Kazuhiko Koike

Molecular biology of the cell 25:302-17 PubMed24258023

2013

Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells.

Applications

Unspecified application

Species

Human

Lina Marcela Gallego-Paez,Hiroshi Tanaka,Masashige Bando,Motoko Takahashi,Naohito Nozaki,Ryuichiro Nakato,Katsuhiko Shirahige,Toru Hirota

Journal of cell science 126:4239-52 PubMed23843628

2013

Dynamic localization of SMC5/6 complex proteins during mammalian meiosis and mitosis suggests functions in distinct chromosome processes.

Applications

Unspecified application

Species

Unspecified reactive species

Rocío Gómez,Philip W Jordan,Alberto Viera,Manfred Alsheimer,Tomoyuki Fukuda,Rolf Jessberger,Elena Llano,Alberto M Pendás,Mary Ann Handel,José A Suja

Neuro-oncology 10:45-51 PubMed18182627

2008

Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Chunyue Yin,Hiroaki Okamoto,Harry Mushlin,Brian M Balgley,Cheng S Lee,Kristy Yuan,Barbara Ikejiri,Sven Glasker,Alexander O Vortmeyer,Edward H Oldfield,Robert J Weil,Zhengping Zhuang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMC5 antibody

Immunoprecipitation - Anti-SMC5 antibody (AB18038)

Western blot - Anti-SMC5 antibody (AB18038)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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