Rabbit Polyclonal SMC6 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMC6 aa 500-800.
Images
Publications
- Experimental and therapeutic medicine 22:12112021HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYayun Lin,Yan Liu,Dongping Xu,Fanfan Guo,Wentao Zhang,Yidan Zhang,Guiqin BaiPubMed 34584556
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SMC6 aa 500-800. The exact immunogen used to generate this antibody is proprietary information. Database link Q96SB8
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Suggested antigen retrievalusing heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541).
Alternative names
Structural maintenance of chromosomes protein 6, SMC protein 6, SMC-6, hSMC6, SMC6L1, SMC6
Recommended products
Rabbit Polyclonal SMC6 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMC6 aa 500-800.
Alternative names
Structural maintenance of chromosomes protein 6, SMC protein 6, SMC-6, hSMC6, SMC6L1, SMC6
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SMC6 aa 500-800. The exact immunogen used to generate this antibody is proprietary information. Database link Q96SB8
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Keep as a concentrated solution.
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Supplementary info
- Activity summary
SMC6 also known as Structural Maintenance of Chromosomes 6 is a core component of the SMC5/6 complex. It has a molecular mass of about 128 kDa. This protein is expressed in various tissues indicating its widespread role in cellular functions. SMC6 is known for its role in DNA repair and proper chromosome segregation during cell division where it maintains genomic stability.
- Biological function summary
SMC6 operates as part of the SMC5/6 complex important for DNA repair processes. The complex is involved in Homologous Recombination Repair (HRR) of DNA double-strand breaks. It also contributes to resolving DNA replication stress. SMC6 ensures that cells sustain genomic integrity by supporting chromosomal dynamics during the cell cycle.
- Pathways
The SMC6 protein is significant in DNA damage response and cell cycle checkpoint pathways. It cooperates closely with related proteins like RAD51 and BRCA1. These interactions are essential in maintaining genomic stability aiding in DNA repair and safe replication processes.
- Associated diseases and disorders
Disruption in SMC6 functions relates to cancer and Fanconi anemia. Its role in maintaining DNA integrity associates it with tumor suppression where impaired SMC6 can lead to genomic instability seen in cancers. In Fanconi anemia faulty coordination with other proteins such as FANCD2 affects DNA repair making SMC6 an important target to better understand these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-SMC6 antibody (ab155495)
7.5% SDS PAGE
All lanes: Western blot - Anti-SMC6 antibody (ab155495) at 1/1000 dilution
All lanes: NT2D1 whole cell lysate at 30 µg
Predicted band size: 126 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SMC6 antibody (ab155495)
Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling SMC6 with ab155495 at 1/200 dilution. Lower panel co-stained with Hoescht 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC6 antibody (ab155495)
Immunohistochemical analysis of paraffin-embedded ES2 xenograft tissue labeling SMC6 with ab155495 at 1/500 dilution.
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