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AB155495

Anti-SMC6 antibody

0

(1 Review)

|

(1 Publication)

Rabbit Polyclonal SMC6 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMC6 aa 500-800.

View Alternative Names

SMC6L1, SMC6, Structural maintenance of chromosomes protein 6, SMC protein 6, SMC-6, hSMC6

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SMC6 antibody (AB155495)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SMC6 antibody (AB155495)

Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling SMC6 with ab155495 at 1/200 dilution. Lower panel co-stained with Hoescht 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC6 antibody (AB155495)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC6 antibody (AB155495)

Immunohistochemical analysis of paraffin-embedded ES2 xenograft tissue labeling SMC6 with ab155495 at 1/500 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SMC6 aa 500-800. The exact immunogen used to generate this antibody is proprietary information.

Q96SB8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrievalusing heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Keep as a concentrated solution.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMC6 also known as Structural Maintenance of Chromosomes 6 is a core component of the SMC5/6 complex. It has a molecular mass of about 128 kDa. This protein is expressed in various tissues indicating its widespread role in cellular functions. SMC6 is known for its role in DNA repair and proper chromosome segregation during cell division where it maintains genomic stability.
Biological function summary

SMC6 operates as part of the SMC5/6 complex important for DNA repair processes. The complex is involved in Homologous Recombination Repair (HRR) of DNA double-strand breaks. It also contributes to resolving DNA replication stress. SMC6 ensures that cells sustain genomic integrity by supporting chromosomal dynamics during the cell cycle.

Pathways

The SMC6 protein is significant in DNA damage response and cell cycle checkpoint pathways. It cooperates closely with related proteins like RAD51 and BRCA1. These interactions are essential in maintaining genomic stability aiding in DNA repair and safe replication processes.

Disruption in SMC6 functions relates to cancer and Fanconi anemia. Its role in maintaining DNA integrity associates it with tumor suppression where impaired SMC6 can lead to genomic instability seen in cancers. In Fanconi anemia faulty coordination with other proteins such as FANCD2 affects DNA repair making SMC6 an important target to better understand these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed : 26983541).
See full target information SMC6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:1211 PubMed34584556

2021

HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yayun Lin,Yan Liu,Dongping Xu,Fanfan Guo,Wentao Zhang,Yidan Zhang,Guiqin Bai
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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