AB202283

Anti-SMCR8 antibody

Name: Anti-SMCR8 antibody (ab202283) | Abcam
Brand: Abcam Limited
SKU: AB202283
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal SMCR8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SMCR8 aa 600 to C-terminus.

View Alternative Names

Guanine nucleotide exchange protein SMCR8, Smith-Magenis syndrome chromosomal region candidate gene 8 protein, SMCR8

2 Images

Supplier Data

Immunoprecipitation - Anti-SMCR8 antibody (AB202283)

Lane 1 : Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognized an epitope of SMCR8.
Lane 2 : Detection of Human SMCR8 by Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202283 at 6 μg per reaction for IP.
Lane 3 : Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab202283 at 0.4 μg/ml.

Detection : Chemiluminescence with an exposure time of 3
minutes.

All lanes:

Immunoprecipitation - Anti-SMCR8 antibody (ab202283)

Predicted band size: 105 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-SMCR8 antibody (AB202283)

Western blot analysis labeling SMCR8 with ab202283 at 0.1 ug/ml.

All lanes:

Western blot - Anti-SMCR8 antibody (ab202283) at 0.1 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Predicted band size: 105 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SMCR8 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TEV9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab202283 was affinity purified using an epitope specific to SMCR8 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMCR8 also known as Smith-Magenis Syndrome Chromosome Region 8 is a protein with a molecular weight of approximately 105 kDa. This protein is expressed in multiple tissues with significant presence in the brain kidney and liver. SMCR8 functions mechanically as a guanine nucleotide exchange factor and it plays a vital role in cellular homeostasis and autophagy.

Biological function summary

SMCR8 is involved in regulating macroautophagy a critical process for cellular maintenance by forming a complex with the C9orf72 protein. This complex ensures proper lysosomal positioning and function impacting the cell's ability to remove damaged organelles and proteins. SMCR8’s activity is essential for maintaining normal lysosomal dynamics and preventing the accumulation of toxic substances within the cell.

Pathways

SMCR8 participates in the autophagy and lysosome pathways. It interacts with several other proteins including UVRAG and Beclin1 which are important for autophagosome formation and maturation. SMCR8 and its associated pathways are important in coordinating proper cellular degradation and recycling processes ensuring cellular stability and function.

SMCR8 has been connected to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Mutations or dysfunction in SMCR8 can interfere with autophagy contributing to the pathogenesis of these disorders. The interaction between SMCR8 and C9orf72 has been particularly highlighted in relation to ALS as disruptions in this interaction could aggravate disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed : 20562859, PubMed : 27103069, PubMed : 27193190, PubMed : 27559131, PubMed : 27617292, PubMed : 28195531, PubMed : 32303654). In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation (PubMed : 20562859, PubMed : 27103069, PubMed : 27617292, PubMed : 28195531). The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ULK1/ATG1 kinase complex and inhibiting its protein kinase activity (PubMed : 27617292, PubMed : 28195531). As part of the C9orf72-SMCR8 complex, stimulates RAB8A and RAB11A GTPase activity in vitro (PubMed : 32303654). Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates (PubMed : 27559131, PubMed : 28195531). In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes (PubMed : 28195531).

See full target information SMCR8

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e55895 PubMed37317656

2023

C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Jülg,Dieter Edbauer,Christian Behrends

Frontiers in cellular neuroscience 16:1061559 PubMed36619668

2022

Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia S Bauer,Christopher P Webster,Allan C Shaw,Jannigje R Kok,Lydia M Castelli,Ya-Hui Lin,Emma F Smith,Francisco Illanes-Álvarez,Adrian Higginbottom,Pamela J Shaw,Mimoun Azzouz,Laura Ferraiuolo,Guillaume M Hautbergue,Andrew J Grierson,Kurt J De Vos

Acta neuropathologica communications 8:110 PubMed32678027

2020

C9orf72-associated SMCR8 protein binds in the ubiquitin pathway and with proteins linked with neurological disease.

Applications

Unspecified application

Species

Unspecified reactive species

John L Goodier,Alisha O Soares,Gavin C Pereira,Lauren R DeVine,Laura Sanchez,Robert N Cole,Jose Luis García-Pérez

eLife 8: PubMed31612854

2019

Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

Applications

Unspecified application

Species

Unspecified reactive species

Carl Laflamme,Paul M McKeever,Rahul Kumar,Julie Schwartz,Mahshad Kolahdouzan,Carol X Chen,Zhipeng You,Faiza Benaliouad,Opher Gileadi,Heidi M McBride,Thomas M Durcan,Aled M Edwards,Luke M Healy,Janice Robertson,Peter S McPherson

Genes & development 32:929-943 PubMed29950492

2018

The C9orf72-interacting protein Smcr8 is a negative regulator of autoimmunity and lysosomal exocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Zhang,Aaron Burberry,Jin-Yuan Wang,Jackson Sandoe,Sulagna Ghosh,Namrata D Udeshi,Tanya Svinkina,Daniel A Mordes,Joanie Mok,Maura Charlton,Quan-Zhen Li,Steven A Carr,Kevin Eggan

eLife 6: PubMed28195531

2017

Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.

Applications

ICC/IF, WB

Species

Human, Human

Jennifer Jung,Arnab Nayak,Véronique Schaeffer,Tatjana Starzetz,Achim K Kirsch,Stefan Müller,Ivan Dikic,Michel Mittelbronn,Christian Behrends

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SMCR8 antibody

Immunoprecipitation - Anti-SMCR8 antibody (AB202283)

Western blot - Anti-SMCR8 antibody (AB202283)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise