AB70635

Anti-Smek1 antibody

Name: Anti-Smek1 antibody (ab70635) | Abcam
Brand: Abcam Limited
SKU: AB70635

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(4 Publications)

Rabbit Polyclonal Smek1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PPP4R3A.

View Alternative Names

KIAA2010, PP4R3A, SMEK1, MSTP033, PPP4R3A, Serine/threonine-protein phosphatase 4 regulatory subunit 3A, SMEK homolog 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smek1 antibody (AB70635)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smek1 antibody (AB70635)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and breast carcinoma (right) tissues labelling Smek1 with ab70635 at 1/200 (1µg/ml) and 1/80 (2.5µg/ml). Detection : DAB and DyLight® 594-conjugated goat anti-rabbit IgG (1/100).

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Western blot - Anti-Smek1 antibody (AB70635)

All lanes:

Western blot - Anti-Smek1 antibody (ab70635) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 95 kDa

Observed band size: 95 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PPP4R3A. The exact immunogen used to generate this antibody is proprietary information.

Q6IN85

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Smek1 protein also known as SMEK homolog 1 or Protein Phosphatase 4 regulatory subunit 3A (PP4R3A) is involved in the regulation of protein phosphorylation. It has a molecular weight of approximately 97 kDa. Smek1 is expressed in various tissues including the brain and liver suggesting it plays roles in multiple biological functions. It acts by directing phosphatase activity towards specific targets making it essential for several signaling pathways.

Biological function summary

Smek1 contributes to cellular processes through its role as part of the protein phosphatase 4 (PP4) complex. This complex modulates several signaling pathways by dephosphorylating specific substrates which influences cell cycle progression apoptosis and DNA repair. As a regulatory subunit Smek1 enhances the specificity of PP4 to target phosphorylated proteins thereby participating in controlling cellular homeostasis and response to stress.

Pathways

Smek1 has essential roles in the DNA damage response and Wnt signaling. In the DNA damage response Smek1 helps control the dephosphorylation of proteins involved in DNA repair such as those encoded by the RAD51 and ATM genes. Through the Wnt signaling pathway Smek1 affects the regulation of beta-catenin an important mediator in cell growth and differentiation. These pathways show how Smek1 integrates into larger cellular processes by directing enzyme activity in response to various signals.

Smek1 holds significance in neurological and cancer conditions. In particular its role in DNA repair connects it to neurodegenerative diseases where proper repair mechanisms are vital to prevent neuronal damage. Furthermore alterations in Smek1 function have implications in oncogenesis especially where atypical Wnt signaling contributes to tumor development. Smek1 interacts with proteins such as beta-catenin in cancer progression highlighting its potential as a target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA DSB repair.

See full target information PPP4R3A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400584 PubMed39206808

2024

Loss of Smek1 Induces Tauopathy and Triggers Neurodegeneration by Regulating Microtubule Stability.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Nan Duan,Ai Liu,Yue-Qing Sun,Yun-Fang Xie,Shi-Jun Wei,Shang Gao,Yi-Ming Liu,Xi Li,Wen-Jie Sun,Jiang-Xia Li,Chuan-Zhu Yan,Qi-Ji Liu

Disease models & mechanisms 15: PubMed35314861

2022

Ppp4r3a deficiency leads to depression-like behaviors in mice by modulating the synthesis of synaptic proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Gao,Ai Liu,Xing Qi,Meitian Wang,Xiao Chen,Shijun Wei,Shang Gao,Yueqing Sun,Ping Sun,Xi Li,Wenjie Sun,Jiangxia Li,Qiji Liu

Journal of oncology 2021:6617700 PubMed33564307

2021

Exosomal MiR-1290 Promotes Angiogenesis of Hepatocellular Carcinoma via Targeting SMEK1.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Wang,Guanwen Wang,Lianjie Niu,Shaorong Zhao,Jianjun Li,Zhen Zhang,Huimin Jiang,Quansheng Zhang,Hang Wang,Peiqing Sun,Rong Xiang,Antao Chang,Shuang Yang

Oncotarget 7:52255-52269 PubMed27391343

2016

PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-κB signaling in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yi Ho,Chi-Ming Liang,Shu-Mei Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smek1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smek1 antibody (AB70635)

Western blot - Anti-Smek1 antibody (AB70635)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise