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AB224222

Anti-SMEK2 antibody

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(2 Publications)

Rabbit Polyclonal SMEK2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPP4R3B aa 650-750.

View Alternative Names

KIAA1387, PP4R3B, SMEK2, PPP4R3B, Serine/threonine-protein phosphatase 4 regulatory subunit 3B, SMEK homolog 2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (AB224222)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (AB224222)

Paraffin-embedded human testis tissue stained for SMEK2 using ab224222 at 1/50 dilution in immunohistochemical analysis. Shows strong nuclear postitivity in seminiferous ducts cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (AB224222)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (AB224222)

Paraffin-embedded human cerebral cortex tissue stained for SMEK2 using ab224222 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SMEK2 antibody (AB224222)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMEK2 antibody (AB224222)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SMEK2 (green) using ab224222 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (AB224222)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (AB224222)

Paraffin-embedded human fallopian tube tissue stained for SMEK2 using ab224222 at 1/50 dilution in immunohistochemical analysis. Shows strong nuclear positivity in glandular cells.

Western blot - Anti-SMEK2 antibody (AB224222)
  • WB

Supplier Data

Western blot - Anti-SMEK2 antibody (AB224222)

All lanes:

Western blot - Anti-SMEK2 antibody (ab224222) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

EFO-21 cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 97 kDa

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Western blot - Anti-SMEK2 antibody (AB224222)
  • WB

Supplier Data

Western blot - Anti-SMEK2 antibody (AB224222)

All lanes:

Western blot - Anti-SMEK2 antibody (ab224222) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 97 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PPP4R3B aa 650-750. The exact immunogen used to generate this antibody is proprietary information.

Q5MIZ7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMEK2 also known as protein phosphatase 4 regulatory subunit 3B (PPP4R3B) is a regulatory subunit of the protein phosphatase 4 (PP4) complex. SMEK2 exhibits a molecular mass of about 106 kDa. It is distributed in various tissues with notable expression in the brain and skeletal muscle. As part of the PP4 complex its primary role is to guide and regulate the specific functions of the catalytic subunits of the complex.
Biological function summary

SMEK2 regulates cellular processes by modulating phosphatase activity. It acts as part of the PP4 complex which includes the PP4 catalytic subunit and other regulatory subunits. This complex plays a role in diverse biological functions such as cellular response to DNA damage and cell division. By affecting dephosphorylation events SMEK2 influences cellular signaling allowing for precise cellular responses under varying conditions.

Pathways

SMEK2 significantly impacts the DNA damage response and cell cycle regulation pathways. It interacts closely with proteins such as PP4C the catalytic subunit of the PP4 complex and has connections to key checkpoint regulators in these pathways. Such interactions ensure accurate DNA repair and proper progression through cell cycle phases. Changes in SMEK2’s functionality or expression can disrupt these pathways leading to improper cellular responses.

Altered SMEK2 activity or expression has been associated with cancer and neurodegenerative diseases. In cancer SMEK2 along with PP4C influences tumor suppressor pathways by regulating processes like DNA damage repair. Disruption in these interactions can lead to uncontrolled cell proliferation. Additionally abnormalities in SMEK2 have been linked to Alzheimer's disease possibly due to its involvement in neuronal signaling pathways and response to cellular stress.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers.
See full target information PPP4R3B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature 633:932-940 PubMed39232161

2024

CDK5-cyclin B1 regulates mitotic fidelity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Feng Zheng,Aniruddha Sarkar,Humphrey Lotana,Aleem Syed,Huy Nguyen,Richard G Ivey,Jacob J Kennedy,Jeffrey R Whiteaker,Bartłomiej Tomasik,Kaimeng Huang,Feng Li,Alan D D'Andrea,Amanda G Paulovich,Kavita Shah,Alexander Spektor,Dipanjan Chowdhury

Journal of enzyme inhibition and medicinal chemistry 39:2383886 PubMed39072709

2024

Design, synthesis, and evaluation of novel stilbene derivatives that degrade acidic nucleoplasmic DNA-binding protein 1 (And1) and synergize with PARP1 inhibitor in NSCLC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Leyuan Chen,Zhonghao Ren,Yunze Zhang,Wenbin Hou,Yiliang Li
View all publications
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Product promise

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