Rabbit Polyclonal SMG7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SMG7 aa 700-1050.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SMG7 aa 700-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q92540
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Target data
Function
Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.
Alternative names
C1orf16, EST1C, KIAA0250, SMG7, Nonsense-mediated mRNA decay factor SMG7, SMG-7 homolog, hSMG-7
Recommended products
Rabbit Polyclonal SMG7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SMG7 aa 700-1050.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SMG7 aa 700-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q92540
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMG7 also known as Suppressor with Morphogenetic Effect on Genitalia 7 is a protein involved in the nonsense-mediated mRNA decay (NMD) pathway. This protein has an approximate mass of 120 kDa and is expressed widely in human tissues. SMG7 interacts with phosphorylated SMG1 and forms an important component of the complex responsible for degrading mRNAs containing premature stop codons effectively regulating gene expression by preventing the synthesis of truncated proteins.
- Biological function summary
SMG7 functions as an important part of the surveillance system that maintains the quality of mRNA transcripts. It acts within the NMD complex along with proteins like SMG1 SMG8 and SMG9. This protein binds to protein phosphatase 2A (PP2A) aiding in the dephosphorylation and recycling of NMD components. SMG7 plays an important role in controlling mRNA stability influencing cell survival differentiation and adaptation to stress.
- Pathways
SMG7 actively participates in the nonsense-mediated decay pathway. This pathway is central to maintaining cellular homeostasis by eliminating defective transcripts that could encode malfunctioning proteins. SMG7 often works in conjunction with proteins such as UPF1 and UPF2 sharing roles in recognizing and targeting faulty mRNAs for decay. Through its involvement in the decay process SMG7 helps prevent the accumulation of potentially harmful proteins that arise from erroneous gene expression.
- Associated diseases and disorders
Disruptions in SMG7 function have been linked to cancer and neurodegenerative diseases. Aberrant regulation of NMD can lead to the stabilization of mRNAs harboring premature stop codons contributing to tumorigenesis and disease progression. Furthermore SMG7 and proteins like UPF1 have been studied in the context of cancers whereby altered NMD activity might affect cancer cell survival and invasion. Research also indicates the importance of proper NMD function in managing neurological disorders highlighting the potential of targeting SMG7 for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SMG7 antibody (ab126984)
5% SDS PAGE
All lanes: Western blot - Anti-SMG7 antibody (ab126984) at 1/1000 dilution
All lanes: NT2D1 cell lysate at 30 µg
Predicted band size: 118 kDa, 126 kDa, 127 kDa, 142 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG7 antibody (ab126984)
ab126984 at a 1/500 dilution staining SMG7 in paraffin-embedded HeLa xenograft tissue by Immunohistochemistry.
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