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AB204555

Anti-SMIM12 antibody

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(2 Publications )

Rabbit Polyclonal SMIM12 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMIM12 aa 1 to C-terminus.

View Alternative Names

C1orf212, SMIM12, Small integral membrane protein 12

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMIM12 antibody (AB204555)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMIM12 antibody (AB204555)

Paraffin embedded human cerebral cortex tissue stained for SMIM12 using ab204555 at 1/10000 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SMIM12 antibody (AB204555)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMIM12 antibody (AB204555)

SK-MEL-30 cells stained for SMIM12 (green) using ab204555 at 2 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMIM12 antibody (AB204555)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMIM12 antibody (AB204555)

Paraffin embedded human skeletal muscle tissue stained for SMIM12 using ab204555 at 1/10000 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMIM12 antibody (AB204555)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMIM12 antibody (AB204555)

Paraffin embedded human testis tissue stained for SMIM12 using ab204555 at 1/10000 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SMIM12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96EX1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMIM12 also known as Small Integral Membrane Protein 12 is a protein with a mass of approximately 13 kDa. This protein integrates into cellular membranes suggesting it plays a role in membrane-related processes. SMIM12 exhibits expression in many tissues with notable expression in the testes and liver. Its localization in these areas indicates its involvement in specific physiological processes relevant to these organs.
Biological function summary

SMIM12 contributes to cellular mechanisms by participating in signaling functions related to membrane integrity and transport. It appears to be part of a larger protein complex indicating it interacts with other membrane proteins to coordinate cellular activities. Such interactions might suggest it facilitates communication between intra- and extracellular environments important for maintaining normal cell functionality.

Pathways

Understood functions of SMIM12 place it within the cellular signaling pathways linked to membrane integrity and protein transport. It interacts closely with proteins such as CD47 and integrins which are important for cell adhesion and immune response signaling. These pathways ensure cellular communication and proper response to physiological changes.

SMIM12 shows relevance to conditions like testicular dysfunction and liver disease. Its interaction with proteins like integrins suggests a role in disorders affecting membrane stability and cellular adhesion. Understanding these interactions could provide insights into pathological conditions resulting from disrupted membrane functions and aid in the development of new therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SMIM12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:1304-1320.e16 PubMed38382526

2024

Cullin-RING ligases employ geometrically optimized catalytic partners for substrate targeting.

Applications

Unspecified application

Species

Unspecified reactive species

Jerry Li,Nicholas Purser,Joanna Liwocha,Daniel C Scott,Holly A Byers,Barbara Steigenberger,Spencer Hill,Ishita Tripathi-Giesgen,Trent Hinkle,Fynn M Hansen,J Rajan Prabu,Senthil K Radhakrishnan,Donald S Kirkpatrick,Kurt M Reichermeier,Brenda A Schulman,Gary Kleiger

Cardiovascular therapeutics 2022:9966306 PubMed35360546

2022

Upregulated miR-206 Aggravates Deep Vein Thrombosis by Regulating GJA1-Mediated Autophagy of Endothelial Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Jingping Ge,Yuanyuan Yin,Ruowen Yang,Jie Kong,Jianping Gu
View all publications
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Product promise

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