Rabbit Polyclonal SMN/Gemin 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMN1 aa 1-200.
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- Frontiers in genetics 11:5632652021Downregulated microRNA-129-5p by Long Non-coding RNA NEAT1 Upregulates PEG3 Expression to Aggravate Non-alcoholic Steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi Zhang,Huiqing Wen,Bangjian Peng,Jun Weng,Fanhong ZengPubMed 33574830
- Molecular therapy. Nucleic acids 19:1237-12492020MSC-Secreted Exosomal H19 Promotes Trophoblast Cell Invasion and Migration by Downregulating let-7b and Upregulating FOXO1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Chen,Haiyan Ding,Min Wei,Wenhui Zha,Shuang Guan,Ning Liu,Yang Li,Yuan Tan,Yan Wang,Fuju WuPubMed 32069774
- The international journal of biochemistry & cell biology 102:59-702018MicroRNA-17 inhibition overcomes chemoresistance and suppresses epithelial-mesenchymal transition through a DEDD-dependent mechanism in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Mei Wu,Xiao-Wu Hong,Ling-Ling Wang,Xia-Feng Cui,Jun Lu,Gui-Quan Chen,Yuan-Lin ZhengPubMed 29953965
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SMN1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q16637
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Macaque monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Macaque monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Macaque monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Macaque monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed:18984161, PubMed:9845364). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed:18984161). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:18984161). To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate (PubMed:18984161). Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits (PubMed:17178713, PubMed:21816274, PubMed:22101937). Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP (PubMed:31799625). Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development (PubMed:23063131). Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (PubMed:26700805). May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
Alternative names
SMN, SMNT, SMN2, SMNC, SMN1, Survival motor neuron protein, Component of gems 1, Gemin-1
Recommended products
Rabbit Polyclonal SMN/Gemin 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMN1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SMN1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q16637
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMN also known as Survival of Motor Neuron protein or Gemin 1 is an important protein involved in the assembly of small nuclear ribonucleoproteins (snRNPs). It has a molecular mass of approximately 38 kDa. SMN proteins are expressed in almost all tissues with highest levels in spinal cord and brain tissues. They play a mechanical role in Gemin complex assisting RNA processing tasks which are essential in RNA metabolism alongside other Gemin proteins.
- Biological function summary
The SMN protein operates within the SMN-Gemin complex a multi-protein assembly important for snRNP biogenesis and pre-mRNA splicing. This complex coordinates the maturation and transport of snRNPs facilitating the processing of precursor mRNAs in the nucleus. SMN and associated Gemin proteins mediate the assembly of Sm proteins onto snRNA ensuring accurate splicing mechanisms necessary for gene expression.
- Pathways
The SMN protein is notably involved in the chaperone-assisted assembly pathway related to snRNP biogenesis. It plays a central role in the spliceosome assembly process working closely with Sm proteins and other RNA-binding proteins. Importantly SMN interacts with the ribonucleoprotein complex influencing mRNA splicing which integrates into the broader cell signaling pathways such as the cholinergic agonist pathways and RNA splicing pathways that regulate neuromuscular communication.
- Associated diseases and disorders
The deficiency or malfunction of SMN protein directly relates to Spinal Muscular Atrophy (SMA) an autosomal recessive disorder. SMA is characterized by motor neuron degeneration which can be attributed to insufficient SMN protein levels leading to compromised snRNP assembly and splicing. The SMN1 gene deletion or mutation reduces the available SMN protein linking this defect with the severity of SMA. Studies suggest that altering the expression levels of the related SMN2 gene can potentially modulate disease outcomes further emphasizing the connection between SMN protein and SMA pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-SMN/Gemin 1 antibody (ab223068)
All lanes: Western blot - Anti-SMN/Gemin 1 antibody (ab223068) at 1/500 dilution
Lane 1: A549 (human lung carcinoma cell line) whole cell lysate
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody (ab223068)
Paraffin-embedded human testis tissue stained for SMN/Gemin 1 using ab223068 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SMN/Gemin 1 antibody (ab223068)
PFA-fixed, Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SMN/Gemin 1(green) using ab223068 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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