Anti-SMO antibody [HL2975] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-SMO antibody [HL2975] - BSA and Azide free (AB325873)

Boiled and unboiled wild-type (WT) and Smoothened homolog knockout (KO) T-47D cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325873 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

true

• WB

Supplier Data

Western blot - Anti-SMO antibody [HL2975] - BSA and Azide free (AB325873)

Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325873 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE : whole cell extract; ME : membrane extract)

All lanes:

Western blot - Anti-SMO antibody [HL2975] - BSA and Azide free (ab325873) at 1/1000 dilution

Lane 1:

Boiled SH-SY5Y whole cell extracts at 30 µg

Lane 2:

Boiled SH-SY5Y membrane extracts at 30 µg

Lane 3:

Unboiled SH-SY5Y whole cell extracts at 30 µg

Lane 4:

Unboiled SH-SY5Y membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-SMO antibody [HL2975] - BSA and Azide free (AB325873)

Boiled and unboiled mouse brain extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325873 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-SMO antibody [HL2975] - BSA and Azide free (ab325873) at 1/1000 dilution

Lane 1:

Boiled mouse brain extract at 50 µg

Lane 2:

Unboiled mouse brain extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SMO antibody [HL2975] - BSA and Azide free (AB325873)

ab325873 detects Smoothened homolog protein by flow cytometry analysis. Sample : Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with ab325873 diluted at 1 : 50 or a Rabbit IgG isotype control at 4ºC for 1 hour.

• WB

Supplier Data

Western blot - Anti-SMO antibody [HL2975] - BSA and Azide free (AB325873)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325873 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SMO antibody [HL2975] - BSA and Azide free (ab325873) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged SMO transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged SMO transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• Array

Supplier Data

Array - Anti-SMO antibody [HL2975] - BSA and Azide free (AB325873)

ab325873 specifically detects Smoothened homolog protein on a VirDTM-GPCR array. Array was probed with ab325873 at a 1 : 2000 dilution. Yellow : SMO.