AB200219

Anti-SMOC1 antibody

Name: Anti-SMOC1 antibody (ab200219) | Abcam
Brand: Abcam Limited
SKU: AB200219

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(5 Publications)

Rabbit Polyclonal SMOC1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPARC-related modular calcium-binding protein 1 aa 150 to C-terminus.

View Alternative Names

SPARC-related modular calcium-binding protein 1, Secreted modular calcium-binding protein 1, SMOC-1, SMOC1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMOC1 antibody (AB200219)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMOC1 antibody (AB200219)

Immunohistochemical analysis of formalin fixed, paraffin embedded human fetal stomach tissue labeling SMOC1 with ab200291 at 1/100.

Supplier Data

Western blot - Anti-SMOC1 antibody (AB200219)

All lanes:

Western blot - Anti-SMOC1 antibody (ab200219) at 1/500 dilution

All lanes:

OV2008 cell lysate

Predicted band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SPARC-related modular calcium-binding protein 1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H4F8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPARC-related modular calcium binding 1 (SMOC1) is an extracellular matrix protein coded by the SMOC1 gene. Known also as its full form the protein weighs approximately 59 kDa and consists of several domains including a thyroglobulin type-1 repeat and two EF-hand domains. SMOC1 is widely expressed in various tissues such as the eye bone and skin. Its expression pattern suggests it plays an important role in tissue development and maintenance.

Biological function summary

SMOC1 functions involve regulation of angiogenesis and cellular differentiation. It interacts with the extracellular matrix modulating the assembly and dynamics of structural proteins. SMOC1 sounds interactions with calmodulin and integrins forming part of a larger complex. This complex engagement influences cellular processes like cell migration adhesion and stabilization which are pivotal for development and wound healing.

Pathways

SMOC1 associates with integrin signaling and TGF-beta signaling pathways. These pathways govern processes like cell growth apoptosis and differentiation. SMOC1 modulates the activity of integrins connecting with proteins such as integrin alpha-V beta-3 and transforming growth factor beta (TGF-β). Their relationships indicate SMOC1's involvement in cellular communication and response to environmental changes.

SMOC1 links to conditions like microphthalmia and coloboma as well as bone development disorders. Mutations or dysregulation of SMOC1 impairs normal development indicating its role in ocular and skeletal health. Researchers note connections with proteins like Pax6 which is also involved in eye development. This involvement shows how closely SMOC1 is tied to disorders affecting vision and structural integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation.

See full target information SPARC-related modular calcium-binding protein 1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 148:72 PubMed39585417

2024

SMOC1 colocalizes with Alzheimer's disease neuropathology and delays Aβ aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Kaleah Balcomb,Caitlin Johnston,Tomas Kavanagh,Dominique Leitner,Julie Schneider,Glenda Halliday,Thomas Wisniewski,Margaret Sunde,Eleanor Drummond

F1000Research 12:1279 PubMed39291144

2024

Identification of high-performing antibodies for SPARC-related modular calcium-binding protein 1 (SMOC-1) for use in Western Blot and immunoprecipitation.

Applications

Species

Human

Riham Ayoubi,Sara González Bolívar,Michael Nicouleau,Kathleen Southern,Carl Laflamme

Acta neuropathologica 148:9 PubMed39039355

2024

Differences in the cerebral amyloid angiopathy proteome in Alzheimer's disease and mild cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique Leitner,Tomas Kavanagh,Evgeny Kanshin,Kaleah Balcomb,Geoffrey Pires,Manon Thierry,Jianina I Suazo,Julie Schneider,Beatrix Ueberheide,Eleanor Drummond,Thomas Wisniewski

Oncology reports 41:789-800 PubMed30535441

2018

lincRNA‑p21 inhibits the progression of non‑small cell lung cancer via targeting miR‑17‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Ao,Ming Jiang,Jie Zhou,Hongling Liang,Haoming Xia,Gang Chen

Oncology letters 16:2903-2910 PubMed30127878

2018

SMOC1 silencing suppresses the angiotensin II-induced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yize Wang,Xianming Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMOC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMOC1 antibody (AB200219)

Western blot - Anti-SMOC1 antibody (AB200219)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise