Skip to main content

Rabbit Recombinant Monoclonal SMOC1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (AB313570), expandable thumbnail
  • Immunoprecipitation - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (AB313570), expandable thumbnail
  • Immunoprecipitation - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (AB313570), expandable thumbnail
  • Western blot - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (AB313570), expandable thumbnail
  • Western blot - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (AB313570), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IPIHC-PIHC-Fr
Human
Tested
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation.

Alternative names

SPARC-related modular calcium-binding protein 1, Secreted modular calcium-binding protein 1, SMOC-1, SMOC1

Recommended products

Rabbit Recombinant Monoclonal SMOC1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26922-29
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SPARC-related modular calcium binding 1 (SMOC1) is an extracellular matrix protein coded by the SMOC1 gene. Known also as its full form the protein weighs approximately 59 kDa and consists of several domains including a thyroglobulin type-1 repeat and two EF-hand domains. SMOC1 is widely expressed in various tissues such as the eye bone and skin. Its expression pattern suggests it plays an important role in tissue development and maintenance.

Biological function summary

SMOC1 functions involve regulation of angiogenesis and cellular differentiation. It interacts with the extracellular matrix modulating the assembly and dynamics of structural proteins. SMOC1 sounds interactions with calmodulin and integrins forming part of a larger complex. This complex engagement influences cellular processes like cell migration adhesion and stabilization which are pivotal for development and wound healing.

Pathways

SMOC1 associates with integrin signaling and TGF-beta signaling pathways. These pathways govern processes like cell growth apoptosis and differentiation. SMOC1 modulates the activity of integrins connecting with proteins such as integrin alpha-V beta-3 and transforming growth factor beta (TGF-β). Their relationships indicate SMOC1's involvement in cellular communication and response to environmental changes.

Associated diseases and disorders

SMOC1 links to conditions like microphthalmia and coloboma as well as bone development disorders. Mutations or dysregulation of SMOC1 impairs normal development indicating its role in ocular and skeletal health. Researchers note connections with proteins like Pax6 which is also involved in eye development. This involvement shows how closely SMOC1 is tied to disorders affecting vision and structural integrity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570)

    This data was developed using Anti-SMOC1 antibody [EPR26922-29] ab313569, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SMOC1 with Anti-SMOC1 antibody [EPR26922-29] ab313569 at 1/100 (5.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunoprecipitation - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570), expandable thumbnail
    The images were kindly provided by YCharOS Antibody Characterization.

    Immunoprecipitation - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570)

    This data was developed using Anti-SMOC1 antibody [EPR26922-29] ab313569, the same antibody clone in a different buffer formulation.
    SMOC1 was immunoprecipitated from 0.35 mg Wild-type HeLa (human cervical adenocarcinoma epithelial cell) concentrated culture supernatant (ultrafiltration). with Anti-SMOC1 antibody [EPR26922-29] ab313569 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SMOC1 antibody [EPR26922-29] ab313569 at 1/1000 dilution.
    Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) concentrated culture supernatant (ultrafiltration).
    Lane 2: Anti-SMOC1 antibody [EPR26922-29] ab313569 IP in Wild-type HeLa (human cervical adenocarcinoma epithelial cell) concentrated culture supernatant (ultrafiltration).
    Lane 3:Wild-type HeLa (human cervical adenocarcinoma epithelial cell) concentrated culture supernatant (ultrafiltration).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    The images were kindly provided by YCharOS Antibody Characterization.

    All lanes: Immunoprecipitation - Anti-SMOC1 antibody [EPR26922-29] (Anti-SMOC1 antibody [EPR26922-29] ab313569) at 1/30 dilution

    All lanes: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) concentrated culture supernatant (ultrafiltration).

  • Immunoprecipitation - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570), expandable thumbnail

    Immunoprecipitation - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570)

    This data was developed using Anti-SMOC1 antibody [EPR26922-29] ab313569, the same antibody clone in a different buffer formulation.
    SMOC1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) treated with 300 ng/ml Brefeldin A for 24 hours whole cell lysate with Anti-SMOC1 antibody [EPR26922-29] ab313569 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SMOC1 antibody [EPR26922-29] ab313569 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) treated with 300 ng/ml Brefeldin A for 24 hours whole cell lysate
    Lane 2: Anti-SMOC1 antibody [EPR26922-29] ab313569 IP in HeLa (human cervical adenocarcinoma epithelial cell) treated with 300 ng/ml Brefeldin A for 24 hours whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SMOC1 antibody [EPR26922-29] ab313569 in HeLa treated with 300 ng/ml Brefeldin A for 24 hours whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 50 seconds

    All lanes: Immunoprecipitation - Anti-SMOC1 antibody [EPR26922-29] (Anti-SMOC1 antibody [EPR26922-29] ab313569) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) treated with 300 ng/ml Brefeldin A for 24 hours whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 50s

  • Western blot - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570), expandable thumbnail
    The images were kindly provided by YCharOS Antibody Characterization.

    Western blot - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570)

    This data was developed using Anti-SMOC1 antibody [EPR26922-29] ab313569, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Cells were grown for 24 hours in serum free media before the media was collected.
    The images were kindly provided by YCharOS Antibody Characterization.
    Exposure time: 2 minutes

    All lanes: Western blot - Anti-SMOC1 antibody [EPR26922-29] (Anti-SMOC1 antibody [EPR26922-29] ab313569) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) cture supernatant (trafiltration) at 30 µg

    Lane 2: SMOC1 knockout HeLa cture supernatant (trafiltration) at 30 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Observed band size: 50-60 kDa

    Exposure time: 2min

  • Western blot - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570), expandable thumbnail

    Western blot - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570)

    This data was developed using Anti-SMOC1 antibody [EPR26922-29] ab313569, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 48 seconds

    All lanes: Western blot - Anti-SMOC1 antibody [EPR26922-29] (Anti-SMOC1 antibody [EPR26922-29] ab313569) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa treated with /ml Brefeldin A for 24 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50-60 kDa

    Exposure time: 48s

  • Flow Cytometry (Intracellular) - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SMOC1 antibody [EPR26922-29] - BSA and Azide free (ab313570)

    This data was developed using Anti-SMOC1 antibody [EPR26922-29] ab313569, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SMOC1 with Anti-SMOC1 antibody [EPR26922-29] ab313569 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com