Anti-smooth muscle Myosin heavy chain 11 antibody

• WB

Unknown

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody (AB82541)

All lanes:

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1 µg/mL

Lane 1:

PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate at 10 µg

Lane 2:

HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg

Lane 3:

Human bladder tissue lysate - soluble fraction (female, 45 years) (<a href='/en-us/products/unavailable/human-bladder-tissue-lysate-soluble-fraction-female-45-years-ab44654'>ab44654</a>) at 10 µg

Lane 4:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 227 kDa

Observed band size: 227 kDa,49 kDa,64 kDa

true

Exposure time: 30s

• ICC

Lab

Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody (AB82541)

ab82541 staining smooth muscle Myosin heavy chain 11 in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab82541 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• WB

AbReview25425****

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody (AB82541)

All lanes:

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1/2000 dilution

All lanes:

whole cell lysate prepared from human PASMC, P6 at 300 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit polyclonal at 1/2000 dilution

Predicted band size: 227 kDa

Observed band size: 100 kDa,200 kDa,227 kDa

true

Exposure time: 20s

Image courtesy of an anonymous Abreview.