AB247958

Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal smooth muscle Myosin heavy chain 11 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

KIAA0866, MYH11, Myosin-11, Myosin heavy chain 11, SMMHC

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

This data was developed using ab124679, the same antibody clone in a different buffer formulation.

Unpurified ab124679, at 1/100 dilution, staining smooth muscle Myosin heavy chain 11 in paraffin-embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

This data was developed using ab124679, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human smooth muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

This data was developed using ab124679, the same antibody clone in a different buffer formulation.

Unpurified ab124679, at 1/100 dilution, staining smooth muscle Myosin heavy chain 11 in paraffin-embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

This data was developed using ab124679, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Unknown

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

This data was developed using ab124679, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (<a href='/en-us/products/primary-antibodies/smooth-muscle-myosin-heavy-chain-11-antibody-epr5335-ab124679'>ab124679</a>) at 1/10000 dilution

Lane 1:

Human fetal artery lysate at 10 µg

Lane 2:

Human bladder lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 227 kDa

Observed band size: 250 kDa

false

Lab

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

This data was developed using ab124679, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

human fetal artery lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 227 kDa

Observed band size: 250 kDa

false

Lab

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

This data was developed using ab124679, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

mouse spleen at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 227 kDa

Observed band size: 250 kDa

false

OI-RD Scanning - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335]
578 PE

PE Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335]
660 APC

APC Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5335

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab247958 is the carrier-free version of ab124679.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smooth muscle Myosin heavy chain 11 also known as MYH11 or myosin 11 is a protein that plays a mechanical role in muscle contraction. It forms part of the thick filament in smooth muscle cells. MYH11 has a molecular mass of approximately 227 kDa and is expressed in smooth muscle tissues including the walls of blood vessels the gastrointestinal tract and the respiratory system. As a member of the myosin heavy chain family it functions by interacting with actin filaments to facilitate contraction allowing for the maintenance and regulation of vascular tone and organ motility.

Biological function summary

Smooth muscle Myosin heavy chain 11 contributes to muscle function through its role within myofilament complexes. These complexes enable cells to achieve the force generation necessary for muscle contraction. MYH11 is critical to cytoskeletal maintenance and cell movement especially in the smooth muscles that line the visceral organs and blood vessels. Without proper functioning of MYH11 cells may lose structural stability and functionality leading to impaired muscle contraction and vessel elasticity.

Pathways

Smooth muscle Myosin heavy chain 11 engages intimately with signal transduction processes governing muscle contraction and relaxation. MYH11 acts in the RhoA/ROCK signaling pathway which is vital to the regulation of vascular smooth muscle contraction. Within this pathway MYH11 influences the activity of proteins like myosin light chain kinase and phosphatase. The protein also plays a role in actin-myosin complex dynamics through its interaction with cellular elements such as tropomyosin and caldesmon significantly impacting cellular processes like cell proliferation and migration.

Smooth muscle Myosin heavy chain 11 has significant correlations with conditions such as familial thoracic aortic aneurysm and aortic dissection types. Mutations or dysfunctions in MYH11 have a direct impact on the integrity of the aortic wall which can lead to these severe vascular conditions. Additionally MYH11 has associations with hypertension through its involvement in vascular tension regulation. The protein's malfunction may disturb its interactions with other myosin heavy chain family members exacerbating vascular risk factors.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Muscle contraction.

See full target information MYH11

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Artificial cells, nanomedicine, and biotechnology 48:498-505 PubMed32013579

2020

Long non-coding RNA NKILA weakens TNF-α-induced inflammation of MRC-5 cells by miR-21 up-regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Wang,Jiajie Zhang,Yan Sun,Nan Lv,Jianwei Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

OI-RD Scanning - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] - BSA and Azide free (AB247958)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise