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Rabbit Recombinant Monoclonal smooth muscle Myosin heavy chain 11 antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 16 publications.


Images

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (AB133567), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (AB133567), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (AB133567), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (AB133567), expandable thumbnail
  • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (AB133567), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/100-1/250

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Muscle contraction.

Alternative names

KIAA0866, MYH11, Myosin-11, Myosin heavy chain 11, SMMHC

Recommended products

Rabbit Recombinant Monoclonal smooth muscle Myosin heavy chain 11 antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 16 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5336(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Smooth muscle Myosin heavy chain 11 also known as MYH11 or myosin 11 is a protein that plays a mechanical role in muscle contraction. It forms part of the thick filament in smooth muscle cells. MYH11 has a molecular mass of approximately 227 kDa and is expressed in smooth muscle tissues including the walls of blood vessels the gastrointestinal tract and the respiratory system. As a member of the myosin heavy chain family it functions by interacting with actin filaments to facilitate contraction allowing for the maintenance and regulation of vascular tone and organ motility.

Biological function summary

Smooth muscle Myosin heavy chain 11 contributes to muscle function through its role within myofilament complexes. These complexes enable cells to achieve the force generation necessary for muscle contraction. MYH11 is critical to cytoskeletal maintenance and cell movement especially in the smooth muscles that line the visceral organs and blood vessels. Without proper functioning of MYH11 cells may lose structural stability and functionality leading to impaired muscle contraction and vessel elasticity.

Pathways

Smooth muscle Myosin heavy chain 11 engages intimately with signal transduction processes governing muscle contraction and relaxation. MYH11 acts in the RhoA/ROCK signaling pathway which is vital to the regulation of vascular smooth muscle contraction. Within this pathway MYH11 influences the activity of proteins like myosin light chain kinase and phosphatase. The protein also plays a role in actin-myosin complex dynamics through its interaction with cellular elements such as tropomyosin and caldesmon significantly impacting cellular processes like cell proliferation and migration.

Associated diseases and disorders

Smooth muscle Myosin heavy chain 11 has significant correlations with conditions such as familial thoracic aortic aneurysm and aortic dissection types. Mutations or dysfunctions in MYH11 have a direct impact on the integrity of the aortic wall which can lead to these severe vascular conditions. Additionally MYH11 has associations with hypertension through its involvement in vascular tension regulation. The protein's malfunction may disturb its interactions with other myosin heavy chain family members exacerbating vascular risk factors.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567), expandable thumbnail

    Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    All lanes: Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567) at 1/5000 dilution

    All lanes: Human testis lysate at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 227 kDa

    Observed band size: 227 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    Immunocytochemistry/ Immunofluorescence analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/50 dilution (2.34 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/1000 dilution (0.12 µg/mL). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    Immunohistochemical analysis of paraffin-embedded Human ovary tissue labelling smooth muscle Myosin heavy chain 11 with unpurified ab133567 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567), expandable thumbnail

    Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    All lanes: Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567) at 1/1000 dilution

    Lane 1: Human bladder lysate at 10 µg

    Lane 2: Human artery lysate at 10 µg

    Lane 3: Human testis lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 227 kDa

  • Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    Intracellular Flow Cytometry analysis of 293 (Human embryonic kidney epithelial cell) cells labeling smooth muscle Myosin heavy chain 11 (red) with purified ab133567 at a 1/20 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labelling smooth muscle Myosin heavy chain 11 with unpurified ab133567 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    unpurified ab133567 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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