Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (AB240069)

Immunohistochemical analysis of paraffin-embedded Human ovary tissue labelling smooth muscle Myosin heavy chain 11 with unpurified, ab133567 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (AB240069)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/1000 dilution (0.12 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (AB240069)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labelling smooth muscle Myosin heavy chain 11 with unpurified ab133567 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (AB240069)

Immunocytochemistry/ Immunofluorescence analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/50 dilution (2.34 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488 , ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

• ICC/IF

AbReview36508****

Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (AB240069)

Unpurified ab133567 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

This image is courtesy of an anonymous abreview.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (AB240069)

Intracellular Flow Cytometry analysis of 293 (Human embryonic kidney epithelial cell) cells labeling smooth muscle Myosin heavy chain 11 (red) with purified ab133567 at a 1/20 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

• WB

Unknown

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (AB240069)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

All lanes:

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (<a href='/en-us/products/primary-antibodies/smooth-muscle-myosin-heavy-chain-11-antibody-epr5336b-ab133567'>ab133567</a>) at 1/5000 dilution

All lanes:

Human testis lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 227 kDa

Observed band size: 227 kDa

false