Name: Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal
SKU: ab224804
Rating: 4 (2 reviews)

Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804) is a rabbit monoclonal antibody that is used to detect smooth muscle Myosin heavy chain 11 in Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt (Intra)	IHC-P	IHC-Fr
Human	Expected	Tested	Expected
Mouse	Tested	Tested	Tested
Rat	Expected	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/80	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/100	Notes Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information MYH11

Function

Muscle contraction.

Alternative names

KIAA0866, MYH11, Myosin-11, Myosin heavy chain 11, SMMHC

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: SP314
Purification technique: Affinity purification Protein A/G
Concentration
Purification notes: Purified from TCS by protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), in Human, Mouse, Rat samples.

Recommended positive controls

IHC-P: Human small intestine, colon, prostate, fallopian tube and ovary tissues, rat and mouse colon tissue.IHC-Fr: Mouse and rat small intestine tissue. Flow Cyt (Intra): C2C12 cells

Collaboration

Anti-smooth muscle Myosin heavy chain 11 [SP314] - N-terminal (ab224804) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Other related products

We have a range of other formats of antibody clone [SP314] also available for your convenience:
ab224804, Carrier free - ab240983, Alexa Fluor® 488 - ab320557, Alexa Fluor® 647 - ab320558, Alexa Fluor® 555 - ab320559, Alexa Fluor® 594 - ab320560, PE - ab320561, APC - ab320562, Alexa Fluor® 750 - ab321418

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Smooth muscle Myosin heavy chain 11 also known as MYH11 or myosin 11 is a protein that plays a mechanical role in muscle contraction. It forms part of the thick filament in smooth muscle cells. MYH11 has a molecular mass of approximately 227 kDa and is expressed in smooth muscle tissues including the walls of blood vessels the gastrointestinal tract and the respiratory system. As a member of the myosin heavy chain family it functions by interacting with actin filaments to facilitate contraction allowing for the maintenance and regulation of vascular tone and organ motility.

Biological function summary

Smooth muscle Myosin heavy chain 11 contributes to muscle function through its role within myofilament complexes. These complexes enable cells to achieve the force generation necessary for muscle contraction. MYH11 is critical to cytoskeletal maintenance and cell movement especially in the smooth muscles that line the visceral organs and blood vessels. Without proper functioning of MYH11 cells may lose structural stability and functionality leading to impaired muscle contraction and vessel elasticity.

Pathways

Smooth muscle Myosin heavy chain 11 engages intimately with signal transduction processes governing muscle contraction and relaxation. MYH11 acts in the RhoA/ROCK signaling pathway which is vital to the regulation of vascular smooth muscle contraction. Within this pathway MYH11 influences the activity of proteins like myosin light chain kinase and phosphatase. The protein also plays a role in actin-myosin complex dynamics through its interaction with cellular elements such as tropomyosin and caldesmon significantly impacting cellular processes like cell proliferation and migration.

Associated diseases and disorders

Smooth muscle Myosin heavy chain 11 has significant correlations with conditions such as familial thoracic aortic aneurysm and aortic dissection types. Mutations or dysfunctions in MYH11 have a direct impact on the integrity of the aortic wall which can lead to these severe vascular conditions. Additionally MYH11 has associations with hypertension through its involvement in vascular tension regulation. The protein's malfunction may disturb its interactions with other myosin heavy chain family members exacerbating vascular risk factors.

Product promise

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11 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/400 dilution (0.27 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in rat colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224804 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/400 dilution (0.27 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in mouse colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224804 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/100 dilution (1.34 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224804 for 10 mins at room temperature.
Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Immunohistochemistry (Frozen) analysis of mouse small intestine tissue section labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/500 (1.8 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Formalin-fixed, paraffin-embedded human colon tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Immunohistochemistry (Frozen) analysis of rat small intestine tissue section labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/500 (1.8 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Formalin-fixed, paraffin-embedded human small intestine tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Formalin-fixed, paraffin-embedded human prostate tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Formalin-fixed, paraffin-embedded human ovary tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
Flow cytometry analysis of C2C12 (Mouse myoblasts myoblast) labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/80 dilution (1.12 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue.