Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Formalin-fixed, paraffin-embedded human ovary tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/100 dilution (1.34 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224804 for 10 mins at room temperature.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Formalin-fixed, paraffin-embedded human prostate tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Formalin-fixed, paraffin-embedded human colon tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

This data was developed using ab240983, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections Mouse Prostate tissue labelling E Cadherin with ab324191 at 1 : 500 dilution (B), Cytokeratin 5 with ab236216 at 1 : 1500 dilution (C) and smooth muscle Myosin heavy chain 11 with ab240983 at 1 : 4000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-E Cadherin (green; Opal™690), anti-Cytokeratin 5 (magenta; Opal™520) and anti-smooth muscle Myosin heavy chain 11 (gray; Opal™570) on mouse prostate.

Panel B : anti-E Cadherin staining epithelium in mouse prostate.

Panel C : ant-Cytokeratin 5 staining basal cells in mouse prostate.

Panel D : ant-smooth muscle Myosin heavy chain 11 staining smooth muscle in mouse prostate.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324191, ab236216 and ab240983 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Immunohistochemistry (Frozen) analysis of rat small intestine tissue section labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/500 (1.8 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/400 dilution (0.27 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in rat colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224804 for 10 mins at room temperature.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Flow cytometry analysis of C2C12 (Mouse myoblasts myoblast) labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/80 dilution (1.12 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/400 dilution (0.27 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in mouse colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224804 for 10 mins at room temperature.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (AB224804)

Immunohistochemistry (Frozen) analysis of mouse small intestine tissue section labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/500 (1.8 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.