AB212657

Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free

Name: Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free (ab212657) | Abcam
Brand: Abcam Limited
SKU: AB212657
Price: 905 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(5 Publications)

Mouse Monoclonal smooth muscle Myosin heavy chain 11 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Tissue preparation containing MYH11 protein.

View Alternative Names

KIAA0866, MYH11, Myosin-11, Myosin heavy chain 11, SMMHC

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free (AB212657)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free (AB212657)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue section labeling smooth muscle Myosin heavy chain with ab212657 at 1 μg/mL.

IHC-P

AbReview60645****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free (AB212657)

ab212657 staining Smooth muscle myosin heavy chain I in Human lymph node tissue sections by Immunohistochemistry (Formalin/PFA- fixed paraffin embedded section). Tissue was fixed with 10% buffered formalin and blocked with 2% serum for 20 minutes at 22°C; antigen retrieval was by heat mediation in a 10Mm Sodium Citrate (pH6). Permeabilized in 0.2% Tween. Samples were incubated with primary antibody (1/100 dilution in 1% BSA in Tris Buffered Saline) for 1 hour at 22°C. An Alexa Fluor® 594 Donkey anti Mouse IgG (1/200) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Joy Folkvord.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free (AB212657)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Leiomyosarcoma tissue section labeling smooth muscle Myosin heavy chain with ab212657 at 1 μg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SMMS-1

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Tissue preparation containing MYH11 protein. The exact immunogen used to generate this antibody is proprietary information.

P35749

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval using 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.</p><p>Incubate with primary for 30 minutes at room temperature.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab212657 may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smooth muscle myosin heavy chain 1 also known as MYH11 plays a significant role in the contractile function of smooth muscle tissues. It is a part of the myosin II family which are motor proteins responsible for converting chemical energy into mechanical force enabling muscle contraction. MYH11 has a high molecular mass approximately 220 kDa and exists primarily in smooth muscles like those in the walls of blood vessels and the gastrointestinal tract.

Biological function summary

MYH11 functions as a critical part of the contractile apparatus in smooth muscle cells. It interacts with actin filaments to initiate contraction utilizing ATP in the process. MYH11 often forms a complex with other proteins including light chains and myosin-binding proteins to ensure proper muscle contraction and relaxation. This complex stabilizes the filament structure optimizing muscle performance.

Pathways

MYH11 integrates into smooth muscle contraction pathways and the cytoskeletal signaling networks. Its activity involves interaction with the RhoA/Rho kinase pathway essential for muscle contraction regulation. Proteins such as actin and tropomyosin work closely with MYH11 in these pathways facilitating coordinated contraction and enabling cells to adapt to mechanical stress.

MYH11 mutations or dysregulation associate with conditions like familial aortic aneurysm and patent ductus arteriosus. In these disorders the normal contractile function of smooth muscle can be compromised leading to vessel weakening. Additionally MYH11 may involve with proteins like actin and caldesmon in these pathologies where altered contractile proteins contribute to disease progression and symptoms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Muscle contraction.

See full target information MYH11

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401844 PubMed38884204

2024

Versatile Design of NO-Generating Proteolipid Nanovesicles for Alleviating Vascular Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyue Yang,Xiangyun Zhang,Hongyu Yan,Rongping Zhao,Ruixin Zhang,Liuyang Zhu,Jingai Zhang,Adam C Midgley,Ye Wan,Songdi Wang,Meng Qian,Qiang Zhao,Ding Ai,Ting Wang,Deling Kong,Xinglu Huang,Kai Wang

Protein & cell 14:433-447 PubMed37402315

2023

Spatially resolved expression landscape and gene-regulatory network of human gastric corpus epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Dong,Xinglong Wu,Xin Zhou,Yuan Gao,Changliang Wang,Wendong Wang,Weiya He,Jingyun Li,Wenjun Deng,Jiayu Liao,Xiaotian Wu,Yongqu Lu,Antony K Chen,Lu Wen,Wei Fu,Fuchou Tang

Science advances 8:eabl3888 PubMed35294246

2022

Mechanically reinforced biotubes for arterial replacement and arteriovenous grafting inspired by architectural engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Dengke Zhi,Quhan Cheng,Adam C Midgley,Qiuying Zhang,Tingting Wei,Yi Li,Ting Wang,Tengzhi Ma,Muhammad Rafique,Shuang Xia,Yuejuan Cao,Yangchun Li,Jing Li,Yongzhe Che,Meifeng Zhu,Kai Wang,Deling Kong

Journal of biomedical materials research. Part A 109:2493-2505 PubMed34096176

2021

The performance of heparin modified poly(ε-caprolactone) small diameter tissue engineering vascular graft in canine-A long-term pilot experiment in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Ye,Toshitaka Takagi,Chengzhao Tu,Akeo Hagiwara,Xue Geng,Zengguo Feng

Biomaterials 242:119922 PubMed32155476

2020

Construction of vascular graft with circumferentially oriented microchannels for improving artery regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Pingli Wu,Lina Wang,Wen Li,Yu Zhang,Yifan Wu,Dengke Zhi,Hongjun Wang,Lianyong Wang,Deling Kong,Meifeng Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free (AB212657)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free (AB212657)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] - BSA and Azide free (AB212657)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise