AB219652

Anti-Smoothelin antibody [EPR20044]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Smoothelin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

SMSMO, SMTN, Smoothelin

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle of human bladder cancer. Negative staining on tumor cells. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on visceral smooth muscle of human stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle and visceral smooth muscle of human colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle of skeletal muscle. Negative staining on skeletal muscle cells. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (GIST) tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on scattered tumor cells of human gastrointestinal stromal tumor (PMID : 19950405). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle and visceral smooth muscle of mouse colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle and visceral smooth muscle of rat colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Negative control : No staining on rat skeletal muscle (PMID : 8707825).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cardiac vascular smooth muscle cells. Negative staining on myocardium. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Smoothelin antibody [EPR20044] (AB219652)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 minutes; Lane 2 : 3 seconds.

This antibody reacts with the 59 kDa and 110 kDa bands, corresponding to isoforms A and B respectively. Smoothelin is exclusively found in smooth muscle cells (PMID : 8707825).

All lanes:

Western blot - Anti-Smoothelin antibody [EPR20044] (ab219652) at 1/1000 dilution

Lane 1:

Human bladder lysate at 20 µg

Lane 2:

Human uterus lysate at 10 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/4000 dilution

Lane 2:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 99 kDa

Observed band size: 110 kDa,59 kDa

false

Supplier Data

Western blot - Anti-Smoothelin antibody [EPR20044] (AB219652)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 30 seconds; Lane 2 : 2 seconds.

This antibody reacts with a 59 kDa band in mouse and rat colon smooth muscle.

All lanes:

Western blot - Anti-Smoothelin antibody [EPR20044] (ab219652) at 1/1000 dilution

Lane 1:

Mouse colon lysate at 10 µg

Lane 2:

Rat colon lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 99 kDa

Observed band size: 59 kDa

false

Carrier free

Anti-Smoothelin antibody [EPR20044] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20044

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smoothelin is a cytoskeletal protein known for its mechanical role in smooth muscle cells. It is approximately 59 kDa in mass. The protein binds to actin filaments contributing to muscle cell contractility and maintaining the integrity of vascular tissues. It is expressed predominantly in contractile smooth muscle cells in tissues such as the intestines bladder and vasculature. Alternative names for Smoothelin include SMTN and smoothelin-A the latter referring to its variant expressed in some muscle tissues.

Biological function summary

Smoothelin is an important component in smooth muscle contraction processes. It is not typically associated with larger protein complexes instead it functions directly with actin filaments. The protein stabilizes and organizes these filaments facilitating a contracted state that is essential for the function of hollow organs and blood vessels. Through its interactions with actin Smoothelin plays a regulatory role in ensuring the contractile apparatus of smooth muscle tissues functions properly.

Pathways

Smoothelin plays an integral role in the smooth muscle contractile pathway. This pathway includes interactions with other proteins such as myosin light-chain kinase which phosphorylates myosin to facilitate muscle contraction. Additionally Smoothelin is associated with the actin cytoskeleton pathway which orchestrates the organization of the cytoskeleton to manage cellular shape and motility. These pathways highlight the coordination among actin-binding proteins in supporting dynamic cellular functions.

Dysregulation or alteration of Smoothelin expression associates with conditions like atherosclerosis and bladder dysfunction. In atherosclerosis impaired smooth muscle contractility often related to aberrant protein expression contributes to vascular stiffness and plaque formation. Furthermore changes in Smoothelin expression in bladder tissues can link to smooth muscle dysfunction disorders. Smoothelin's activity may influence or be influenced by other proteins involved in these diseases such as myosin light-chain phosphatase which counterbalances kinase activity to regulate contraction levels.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural protein of the cytoskeleton.

See full target information SMTN

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:280 PubMed40887473

2025

Focal adhesion kinase/Src family kinase axis-mediated tyrosine phosphorylation of metabolic enzymes facilitates tumor metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Jing Zhang,Yuheng Zhu,Yanmeng Zhu,Jingyuan Pang,Qingnan Wu,Yan Wang,Qimin Zhan

iScience 27:111255 PubMed39620128

2024

activates SLFN4MDSCs to accelerate gastric intestinal metaplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihong Zhao,Rui Liu,Ye Peng,Cun Li,Qian Li

Nature neuroscience 27:232-248 PubMed38168932

2024

Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling.

Applications

Unspecified application

Species

Unspecified reactive species

Dongdong Zhang,Jiayu Ruan,Shiyu Peng,Jinze Li,Xu Hu,Yiyi Zhang,Tianrui Zhang,Yaping Ge,Zhu Zhu,Xian Xiao,Yunxu Zhu,Xuzhao Li,Tingbo Li,Lili Zhou,Qingzhu Gao,Guoxiao Zheng,Bingrui Zhao,Xiangqing Li,Yanming Zhu,Jinsong Wu,Wensheng Li,Jingwei Zhao,Woo-Ping Ge,Tian Xu,Jie-Min Jia

The American journal of pathology 190:1843-1858 PubMed32479820

2020

M2 Macrophages and Phenotypic Modulation of Intestinal Smooth Muscle Cells Characterize Inflammatory Stricture Formation in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra R Lourenssen,Michael G Blennerhassett

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smoothelin antibody [EPR20044]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (AB219652)

Western blot - Anti-Smoothelin antibody [EPR20044] (AB219652)

Western blot - Anti-Smoothelin antibody [EPR20044] (AB219652)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise