AB235183

Anti-Smoothened antibody

Name: Anti-Smoothened antibody (ab235183) | Abcam
Brand: Abcam Limited
SKU: AB235183
Price: 510 USD
Availability: InStock

(1 Review)

(6 Publications)

Rabbit Polyclonal Smoothened antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

SMOH, SMO, Protein smoothened, Protein Gx

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothened antibody (AB235183)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothened antibody (AB235183)

Paraffin-embedded human lung tissue stained for Smoothened using ab235183 at 1/100 dilution in immunohistochemical analysis. Counterstained with hematoxylin.

ab235183 developed a specific nuclear staining of the alveolar cells in lung tissue.

Supplier Data

Western blot - Anti-Smoothened antibody (AB235183)

Overexpressing lysate expected to run ~100 kDa.

All lanes:

Western blot - Anti-Smoothened antibody (ab235183) at 1/1000 dilution

Lane 1:

Smoothened (C-terminal DDK tagged) overexpressing HEK-293T lysate at 10 µg

Lane 2:

HEK293T lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/70000 dilution

Predicted band size: 86 kDa

false

Exposure time: 9s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smoothened (SMO) is a protein that plays a vital role in the Hedgehog signaling pathway. It is also known by other names such as SMOH and Smoothened homolog. This protein has a molecular mass of approximately 86 kDa. Smoothened is expressed in a variety of tissues including the brain lungs and kidneys. It functions as a transmembrane protein with seven helices which transmits signals across the cell membrane.

Biological function summary

Smoothened influences cellular processes by participating in the Hedgehog signaling pathway. It operates as part of a complex with the Patched (PTCH) receptor. When the Hedgehog ligand binds to PTCH Smoothened becomes activated. This activation leads to changes in gene expression that control cell growth and differentiation. Smoothened is critical in embryonic development tissue regeneration and stem cell maintenance.

Pathways

Smoothened interacts with essential components of the Hedgehog signaling pathway. This pathway includes proteins like PTCH and Gli transcription factors. Smoothened activation triggers the release of these Gli proteins from inhibitory complexes which then migrate to the nucleus to modulate the transcription of target genes. The Hedgehog pathway is important in regulating developmental processes and cellular responses.

Smoothened has connections to various forms of cancer including basal cell carcinoma and medulloblastoma. Abnormal activation or mutations in Smoothened can lead to unchecked cell proliferation. Smoothened also shows relevance in the development of gliomas. Drugs like 'anti smo' or 'glasdegib' act as Smoothened antagonists aiming to inhibit its function in these diseases by targeting the Hedgehog signaling pathway.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed : 19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).

See full target information SMO

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology reports 50:3317-3327 PubMed36725745

2023

Sirt1 regulates microglial activation and inflammation following oxygen-glucose deprivation/reoxygenation injury by targeting the Shh/Gli-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Liao,Jiagui Huang,Jie Liu,Huimin Zhu,Yue Chen,Xuemei Li,Jun Wen,Qin Yang

Journal of ovarian research 15:141 PubMed36581994

2022

GIMAP7 induces oxidative stress and apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inhibiting sonic hedgehog signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Anran Xu,Yuanyuan Fan,Song Liu,Lianbing Sheng,Yanyan Sun,Huijun Yang

BioMed research international 2022:8453011 PubMed36246980

2022

Resveratrol Induces Apoptosis, Suppresses Migration, and Invasion of Cervical Cancer Cells by Inhibiting the Hedgehog Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Jiang,Zhenghua Liu,Xiuping Zhou,Fenglan Peng,Zhihui Wang,Feng Li,Ming Li

Experimental and therapeutic medicine 23:429 PubMed35607374

2022

Expression of yes-associated protein, β-catenin and smoothened, and their clinical significance in invasive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pengju Liu,Jianfeng Zeng,Gaohua Yang

Frontiers in cell and developmental biology 9:716610 PubMed34646822

2021

Sonic Hedgehog Induces Mesenchymal Stromal Cell Senescence-Associated Secretory Phenotype and Chondrocyte Apoptosis in Human Osteoarthritic Cartilage.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Feng,Wenguang Liu,Jing Ding,Yusheng Qiu,Qian Chen

Molecular medicine reports 23: PubMed33398379

2021

Silencing of long non‑coding RNA NEAT1 inhibits hepatocellular carcinoma progression by downregulating SMO by sponging microRNA‑503.

Applications

Unspecified application

Species

Unspecified reactive species

Chuihua Sun,Ting Xiao,Ying Xiao,Yunbao Li

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Smoothened antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothened antibody (AB235183)

Western blot - Anti-Smoothened antibody (AB235183)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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