Anti-Smoothened antibody
1
(1 Review)
|
(28 Publications)
Rabbit Polyclonal Smoothened antibody. Suitable for WB, IF, IHC-P and reacts with Rat samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human SMO aa 500-600.
View Alternative Names
SMOH, SMO, Protein smoothened, Protein Gx
- IF
Supplier Data
Immunofluorescence - Anti-Smoothened antibody (AB236465)
Formalin-fixed, paraffin-embedded rat brain tissue stained for Smoothened using ab236465 at 1/200 dilution in ICC/IF. Secondary antibody was Cy3 conjugated goat anti-rabbit IgG.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothened antibody (AB236465)
Formalin-fixed, paraffin-embedded rat brain tissue stained for Smoothened using ab236465 at 1/200 dilution in immunohistochemical analysis. DAB staining.
- WB
Supplier Data
Western blot - Anti-Smoothened antibody (AB236465)
All lanes:
Western blot - Anti-Smoothened antibody (ab236465) at 1/200 dilution
Lane 1:
Rat kidney lysate
Lane 2:
Rat brain lysate
Predicted band size: 86 kDa
false
Reactivity data
Properties and storage information
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Shipped at conditions
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Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Smoothened influences cellular processes by participating in the Hedgehog signaling pathway. It operates as part of a complex with the Patched (PTCH) receptor. When the Hedgehog ligand binds to PTCH Smoothened becomes activated. This activation leads to changes in gene expression that control cell growth and differentiation. Smoothened is critical in embryonic development tissue regeneration and stem cell maintenance.
Pathways
Smoothened interacts with essential components of the Hedgehog signaling pathway. This pathway includes proteins like PTCH and Gli transcription factors. Smoothened activation triggers the release of these Gli proteins from inhibitory complexes which then migrate to the nucleus to modulate the transcription of target genes. The Hedgehog pathway is important in regulating developmental processes and cellular responses.
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Target data
Publications (28)
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Genes & genomics 47:961-972 PubMed40794264
2025
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International journal of molecular sciences 26: PubMed40332045
2025
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Nature communications 16:1267 PubMed39894896
2025
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The Tohoku journal of experimental medicine : PubMed39603602
2024
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Nature communications 15:987 PubMed38307877
2024
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International journal of molecular medicine 53: PubMed38240085
2024
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Journal of biophotonics 16:e202300043 PubMed37483112
2023
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Neural regeneration research 18:2208-2218 PubMed37056140
2023
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Journal of personalized medicine 12: PubMed36556306
2022
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Journal of translational medicine 20:520 PubMed36348351
2022
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