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AB236465

Anti-Smoothened antibody

1

(1 Review)

|

(28 Publications)

Rabbit Polyclonal Smoothened antibody. Suitable for WB, IF, IHC-P and reacts with Rat samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human SMO aa 500-600.

View Alternative Names

SMOH, SMO, Protein smoothened, Protein Gx

3 Images
Immunofluorescence - Anti-Smoothened antibody (AB236465)
  • IF

Supplier Data

Immunofluorescence - Anti-Smoothened antibody (AB236465)

Formalin-fixed, paraffin-embedded rat brain tissue stained for Smoothened using ab236465 at 1/200 dilution in ICC/IF. Secondary antibody was Cy3 conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothened antibody (AB236465)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothened antibody (AB236465)

Formalin-fixed, paraffin-embedded rat brain tissue stained for Smoothened using ab236465 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Western blot - Anti-Smoothened antibody (AB236465)
  • WB

Supplier Data

Western blot - Anti-Smoothened antibody (AB236465)

All lanes:

Western blot - Anti-Smoothened antibody (ab236465) at 1/200 dilution

Lane 1:

Rat kidney lysate

Lane 2:

Rat brain lysate

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB, IHC-P, IF

applications

Immunogen

Synthetic Peptide within Human SMO aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

Q99835

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smoothened (SMO) is a protein that plays a vital role in the Hedgehog signaling pathway. It is also known by other names such as SMOH and Smoothened homolog. This protein has a molecular mass of approximately 86 kDa. Smoothened is expressed in a variety of tissues including the brain lungs and kidneys. It functions as a transmembrane protein with seven helices which transmits signals across the cell membrane.
Biological function summary

Smoothened influences cellular processes by participating in the Hedgehog signaling pathway. It operates as part of a complex with the Patched (PTCH) receptor. When the Hedgehog ligand binds to PTCH Smoothened becomes activated. This activation leads to changes in gene expression that control cell growth and differentiation. Smoothened is critical in embryonic development tissue regeneration and stem cell maintenance.

Pathways

Smoothened interacts with essential components of the Hedgehog signaling pathway. This pathway includes proteins like PTCH and Gli transcription factors. Smoothened activation triggers the release of these Gli proteins from inhibitory complexes which then migrate to the nucleus to modulate the transcription of target genes. The Hedgehog pathway is important in regulating developmental processes and cellular responses.

Smoothened has connections to various forms of cancer including basal cell carcinoma and medulloblastoma. Abnormal activation or mutations in Smoothened can lead to unchecked cell proliferation. Smoothened also shows relevance in the development of gliomas. Drugs like 'anti smo' or 'glasdegib' act as Smoothened antagonists aiming to inhibit its function in these diseases by targeting the Hedgehog signaling pathway.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed : 19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).
See full target information SMO

Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Genes & genomics 47:961-972 PubMed40794264

2025

NCOA4 inhibits glioma progression by suppressing the Sonic Hedgehog pathway and its overexpression indicates a better glioma prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaining Liu,Hu Wang,Tian Qiu,Guangxiu Wang,Anling Zhang,Zhifan Jia,Xiaoguang Tong

International journal of molecular sciences 26: PubMed40332045

2025

Crosstalk Between Wnt/β-Catenin and Hedgehog Supports Gli1+ Lineage Osteogenesis in Cranial Sutures.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Sun,Jie Wang,Shuo Chen,Yang He

Nature communications 16:1267 PubMed39894896

2025

The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shahid Banday,Alok K Mishra,Romana Rashid,Tianyi Ye,Amjad Ali,Junhui Li,Jason T Yustein,Michelle A Kelliher,Lihua Julie Zhu,Sara K Deibler,Sunil K Malonia,Michael R Green

The Tohoku journal of experimental medicine : PubMed39603602

2024

Yangfei Tongluo Decoction Alleviated Fibrosis and Inflammation in Post-Infectious Bronchiolitis Obliterans via Hedgehog Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxia Chen,Libiao Wang,Sujing Su,Shuhua Fan

Nature communications 15:987 PubMed38307877

2024

Phosphorylation of human glioma-associated oncogene 1 on Ser937 regulates Sonic Hedgehog signaling in medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Hui Zeng,Chao Tang,Minli Yao,Qiangqiang He,Meiyu Qv,Qianlei Ren,Yana Xu,Tingyu Shen,Weizhong Gu,Chengyun Xu,Chaochun Zou,Xing Ji,Ximei Wu,Jirong Wang

International journal of molecular medicine 53: PubMed38240085

2024

NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica‑induced lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhou,Qun Zhang,Chenyang Liu,Jiahao Fan,Wen Huang,Nan Li,Mingxia Yang,Hong Wang,Weiping Xie,Hui Kong

Journal of biophotonics 16:e202300043 PubMed37483112

2023

Regeneration of taste through sonic hedgehog upregulation by photobiomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Young Lee,Celine Dg Abueva,Andrew Padalhin,So Young Park,Hyun Seok Ryu,Phil-Sang Chung,Hee Jung Kim,Jeongyun Kim,Seung Hoon Woo

Neural regeneration research 18:2208-2218 PubMed37056140

2023

M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Gui Huang,Jiang-Xia Ren,Yue Chen,Ming-Fen Tian,Li Zhou,Jun Wen,Xiao-Song Song,You-Lin Wu,Qing-Huan Yang,Pei-Ran Jiang,Jia-Ni Wang,Qin Yang

Journal of personalized medicine 12: PubMed36556306

2022

Resveratrol Inhibits Oxidative Stress and Regulates M1/M2-Type Polarization of Microglia via Mediation of the Nrf2/Shh Signaling Cascade after OGD/R Injury In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Hongyan Liao,Yue Chen,Huimin Zhu,Xuemei Li,Jing Liu,Qin Xiang,Fanling Zeng,Qin Yang

Journal of translational medicine 20:520 PubMed36348351

2022

CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jungang Zhao,Rizhao Li,Jiacheng Li,Ziyan Chen,Zixia Lin,Baofu Zhang,Liming Deng,Gang Chen,Yi Wang
View all publications

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