Rabbit Polyclonal SMOX antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMOX aa 1-150.
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Publications
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- Journal of Cancer 13:3051-30602022Microbiome dysbiosis inhibits carcinogen-induced murine oral tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuh-Ling Chen,Kuan-Chih Huang,Jer-Horng Wu,Tsunglin Liu,Jiung-Wen Chen,Jia-Yan Xie,Meng-Yen Chen,Li-Wha Wu,Chun-Liang TungPubMed 36046649
- Hepatology research : the official journal of the 48:967-9772018Spermine oxidase is upregulated and promotes tumor growth in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Hu et. al.PubMed 29923661
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SMOX aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NWM0
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Cells were fixed in 4% paraformaldehyde and then permealized with 0.1% Triton X-100. |
Associated Products
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Target data
Function
Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.
Alternative names
C20orf16, SMO, UNQ3039/PRO9854, SMOX, Spermine oxidase, Polyamine oxidase 1, PAO-1, PAOh1
Recommended products
Rabbit Polyclonal SMOX antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMOX aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SMOX aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NWM0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMOX also known as spermine oxidase is an enzyme that catalyzes the oxidation of spermine to spermidine releasing hydrogen peroxide and 3-aminopropanal. This enzyme is important in the polyamine metabolism process. SMOX has a molecular mass of approximately 66 kDa. It is expressed in various tissues including the liver kidney and other tissues with high metabolic activity.
- Biological function summary
SMOX is essential for polyamine catabolism influencing cell growth and proliferation. It does not function as part of a complex but works independently to regulate intracellular levels of polyamines. The balance of polyamines like spermine and spermidine is critical for cellular functions impacting cell cycle regulation and apoptosis.
- Pathways
The enzymatic activity of SMOX plays an important role in the polyamine metabolic pathway. This pathway interconnects with the urea cycle and is involved in the regulation of cellular proliferation. SMOX influences the activity of proteins like ornithine decarboxylase (ODC) which is vital in polyamine synthesis. By controlling polyamine levels SMOX indirectly modulates signaling pathways associated with cellular responses and stress reactions.
- Associated diseases and disorders
SMOX has a connection with cancer and neurodegenerative diseases. Abnormal expression of SMOX can disrupt polyamine homeostasis leading to oxidative stress and promoting tumorigenesis. In cancer SMOX activity often pairs with increased levels of ODC contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's disease SMOX activity is linked to elevated oxidative stress resulting in neuronal damage and cognitive decline. The regulation of SMOX and polyamine metabolism remains a focus of research for therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMOX antibody (ab150971)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Spermine oxidase with ab150971 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SMOX antibody (ab150971)
Immunofluorescent analysis of PFA/Triton X-100-fixed U20S cells labeling Spermine oxidase with ab150971 at 4 μg/ml.
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