AB233007

Anti-SMP30 antibody

Name: Anti-SMP30 antibody (ab233007) | Abcam
Brand: Abcam Limited
SKU: AB233007

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(5 Publications)

Rabbit Polyclonal SMP30 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RGN aa 50 to C-terminus.

View Alternative Names

SMP30, RGN, Regucalcin, RC, Gluconolactonase, Senescence marker protein 30, GNL, SMP-30

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMP30 antibody (AB233007)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMP30 antibody (AB233007)

Formalin-fixed, paraffin-embedded human stomach tissue stained for SMP30 using ab233007 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMP30 antibody (AB233007)

Formalin-fixed, paraffin-embedded human liver tissue stained for SMP30 using ab233007 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMP30 antibody (AB233007)

Formalin-fixed, paraffin-embedded human kidney tissue stained for SMP30 using ab233007 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMP30 antibody (AB233007)

Formalin-fixed, paraffin-embedded human liver cancer tissue stained for SMP30 using ab233007 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-SMP30 antibody (AB233007)

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Western blot - Anti-SMP30 antibody (ab233007) at 2 µg/mL

All lanes:

Mouse liver lysate

Predicted band size: 33 kDa

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Supplier Data

Western blot - Anti-SMP30 antibody (AB233007)

All lanes:

Western blot - Anti-SMP30 antibody (ab233007) at 2 µg/mL

All lanes:

Human lung lysate

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-SMP30 antibody (AB233007)

All lanes:

Western blot - Anti-SMP30 antibody (ab233007) at 2 µg/mL

All lanes:

Recombinant human SMP30 protein

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RGN aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15493

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMP30 also known as senescence marker protein-30 or regucalcin functions as a calcium-binding protein involved in calcium homeostasis and antioxidation. SMP30's molecular mass is approximately 36 kDa reflecting its role in cellular maintenance processes. The protein exhibits expression in various tissues including liver kidney brain and lung reflecting its diverse functional roles within the body. SMP30 displays a strong presence in these tissues indicating its involvement in essential cellular mechanisms.

Biological function summary

SMP30 contributes to cellular longevity and protection against oxidative stress. This protein participates in several mechanisms that support cellular senescence and aging processes. SMP30 is part of a family of proteins that control intracellular calcium levels an important factor in cellular signaling and function. By maintaining calcium balance SMP30 enhances cell health and survival especially under conditions that challenge cellular stability.

Pathways

SMP30 plays important roles in the calcium signaling pathway and the oxidative stress response pathway. Within these pathways SMP30 interacts with proteins such as calmodulin and superoxide dismutase. Calmodulin links to calcium signaling while superoxide dismutase addresses oxidative stress management. These interactions place SMP30 centrally within pathways that govern cellular response to physiological and environmental changes.

SMP30 associates with aging-related disorders like osteoporosis and neurodegeneration. Changes in SMP30 expression correlate with altered calcium homeostasis contributing to these conditions. In the context of neurodegeneration SMP30's calcium-binding ability interacts with proteins such as calcium/calmodulin-dependent protein kinase a regulator in neuronal survival and plasticity. Understanding SMP30’s role provides insights into therapeutic avenues especially for diseases where calcium dysregulation and oxidative stress play an important part.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity).

See full target information RGN

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancers 16: PubMed39682121

2024

Downregulated Regucalcin Expression Induces a Cancer-like Phenotype in Non-Neoplastic Prostate Cells and Augments the Aggressiveness of Prostate Cancer Cells: Interplay with the G Protein-Coupled Oestrogen Receptor?

Applications

Unspecified application

Species

Unspecified reactive species

Lara R S Fonseca,Ricardo J P Carreira,Mariana Feijó,José E B Cavaco,Henrique J Cardoso,Cátia V Vaz,Marília I Figueira,Sílvia Socorro

Anais brasileiros de dermatologia 98:17-25 PubMed36244946

2022

Downregulated miR-181a alleviates HO-induced oxidative stress and cellular senescence by targeting PDIA6 in human foreskin fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Huang,Huimin Yan,Yanqing Yang,Jinfei Zhou,Qijun Xu,Hu Meng

Frontiers in genetics 13:902453 PubMed35812737

2022

miR-19b-3p/PKNOX1 Regulates Viral Myocarditis by Regulating Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Jiahui,Zheng Jiadai,Zheng Nan,Zhou Rui,Huang Lipin,He Jian,Zhu Wenzong,Zhang Riyuan

Bioengineered 13:12955-12971 PubMed35615975

2022

Senescence marker protein30 protects lens epithelial cells against oxidative damage by restoring mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

He Teng,Yaru Hong,Jingjing Cao,Hui Li,Fang Tian,Jing Sun,Kai Wen,Guoge Han,Amy Whelchel,Xiaomin Zhang,Xiaorong Li,Lijie Dong

Investigative ophthalmology & visual science 60:4643-4651 PubMed31682715

2019

P38-Mediated Cellular Senescence in Conjunctivochalasis Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Minhong Xiang,Lijuan Mo,Yueping Zhan,Hang Wen,Huanming Zhou,Wanhong Miao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMP30 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMP30 antibody (AB233007)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMP30 antibody (AB233007)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMP30 antibody (AB233007)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMP30 antibody (AB233007)

Western blot - Anti-SMP30 antibody (AB233007)

Western blot - Anti-SMP30 antibody (AB233007)

Western blot - Anti-SMP30 antibody (AB233007)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise