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Rabbit Recombinant Monoclonal SMP30 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-SMP30 antibody [EPR22052-107] - BSA and Azide free (Capture) (AB281164), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity).

Alternative names

SMP30, RGN, Regucalcin, RC, Gluconolactonase, Senescence marker protein 30, GNL, SMP-30

Recommended products

Rabbit Recombinant Monoclonal SMP30 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22052-107
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab281164 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Regucalcin. The recommended pair for sandwich ELISA is:
Capture: ab281164, Human Regucalcin Capture Antibody (unconjugated)
Detector: Anti-SMP30 antibody [EPR22052-155] - BSA and Azide free (Detector) ab281014, Human Regucalcin Detector Antibody (unconjugated)
The reference range value is 78.13-5000 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SMP30 also known as senescence marker protein-30 or regucalcin functions as a calcium-binding protein involved in calcium homeostasis and antioxidation. SMP30's molecular mass is approximately 36 kDa reflecting its role in cellular maintenance processes. The protein exhibits expression in various tissues including liver kidney brain and lung reflecting its diverse functional roles within the body. SMP30 displays a strong presence in these tissues indicating its involvement in essential cellular mechanisms.

Biological function summary

SMP30 contributes to cellular longevity and protection against oxidative stress. This protein participates in several mechanisms that support cellular senescence and aging processes. SMP30 is part of a family of proteins that control intracellular calcium levels an important factor in cellular signaling and function. By maintaining calcium balance SMP30 enhances cell health and survival especially under conditions that challenge cellular stability.

Pathways

SMP30 plays important roles in the calcium signaling pathway and the oxidative stress response pathway. Within these pathways SMP30 interacts with proteins such as calmodulin and superoxide dismutase. Calmodulin links to calcium signaling while superoxide dismutase addresses oxidative stress management. These interactions place SMP30 centrally within pathways that govern cellular response to physiological and environmental changes.

Associated diseases and disorders

SMP30 associates with aging-related disorders like osteoporosis and neurodegeneration. Changes in SMP30 expression correlate with altered calcium homeostasis contributing to these conditions. In the context of neurodegeneration SMP30's calcium-binding ability interacts with proteins such as calcium/calmodulin-dependent protein kinase a regulator in neuronal survival and plasticity. Understanding SMP30’s role provides insights into therapeutic avenues especially for diseases where calcium dysregulation and oxidative stress play an important part.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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