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Rabbit Recombinant Monoclonal SMT3 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Saccharomyces cerevisiae S288C, Transfected cell line - Saccharomyces cerevisiae S288C samples.

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Images

Western blot - Anti-Smt3 antibody [EPR28614-578] (AB322402), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (AB322402), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (AB322402), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (AB322402), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (AB322402), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Transfected cell line - Saccharomyces cerevisiae S288C
Not recommended
Tested
Transfected cell lysate - Saccharomyces cerevisiae S288C
Tested
Not recommended

Tested
Tested

Species
Transfected cell lysate - Saccharomyces cerevisiae S288C
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Saccharomyces cerevisiae S288C
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Saccharomyces cerevisiae S288C
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell lysate - Saccharomyces cerevisiae S288C
Dilution info
-
Notes

-

Target data

Function

Not known; suppressor of MIF2 mutations.

Alternative names

YDR510W, D9719.15, SMT3, Ubiquitin-like protein SMT3

Rabbit Recombinant Monoclonal SMT3 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Saccharomyces cerevisiae S288C, Transfected cell line - Saccharomyces cerevisiae S288C samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28614-578
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Smt3 antibody [EPR28614-578] (ab322402), expandable thumbnail

    Western blot - Anti-Smt3 antibody [EPR28614-578] (ab322402)

    Smt3 Western blot staining using rabbit Anti-Smt3 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-GFP antibody [E385] (Anti-GFP antibody [E385] ab32146) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Smt3 antibody [EPR28614-578] (ab322402) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a GFP-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a Saccharomyces cerevisiae SMT3 expression vector containing a GFP-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 16 kDa, 37 kDa, 36 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (ab322402), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (ab322402)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Smt3 with ab322402 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in rat cerebrum.

    The section was incubated with ab322402 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (ab322402), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (ab322402)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Smt3 with ab322402 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in mouse cerebrum.

    The section was incubated with ab322402 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (ab322402), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (ab322402)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Smt3 with ab322402 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human cerebrum.

    The section was incubated with ab322402 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (ab322402), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] (ab322402)

    Immunohistochemical analysis of paraffin-embedded Panel A 293T (human embryonic kidney epithelial cell) transfected with a Saccharomyces cerevisiae SMT3 expression vector containing a GFP- tag. Panel B 293T cells transfected with empty vector containing a GFP- tag. tissue labeling Smt3 with ab322402 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Saccharomyces cerevisiae SMT3 expression vector containing a GFP tag, no staining in (B) HEK-293T transfected with an empty vector containing a GFP-tag.

    The section was incubated with ab322402 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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