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AB324844

Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free

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Rabbit Recombinant Monoclonal SMT3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Transfected cell line - Saccharomyces cerevisiae S288C, Transfected cell lysate - Saccharomyces cerevisiae S288C samples.

View Alternative Names

YDR510W, D9719.15, SMT3, Ubiquitin-like protein SMT3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)

This data was developed using ab322402, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Panel (A) 293T (human embryonic kidney epithelial cell) transfected with a Saccharomyces cerevisiae SMT3 expression vector containing a GFP- tag and Panel (B) 293T cells transfected with empty vector containing a GFP- tag labeling Smt3 with ab322402 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Saccharomyces cerevisiae SMT3 expression vector containing a GFP tag, no staining in (B) HEK-293T transfected with an empty vector containing a GFP-tag.
The section was incubated with ab322402 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)

This data was developed using ab322402, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Smt3 with ab322402 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human cerebrum.
The section was incubated with ab322402 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)

This data was developed using ab322402, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Smt3 with ab322402 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse cerebrum.
The section was incubated with ab322402 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)

This data was developed using ab322402, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Smt3 with ab322402 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in rat cerebrum.
The section was incubated with ab322402 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)
  • WB

Supplier Data

Western blot - Anti-Smt3 antibody [EPR28614-578] - BSA and Azide free (AB324844)

This data was developed using ab322402, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Smt3 antibody [EPR28614-578] (<a href='/en-us/products/primary-antibodies/smt3-antibody-epr28614-578-ab322402'>ab322402</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Saccharomyces cerevisiae SMT3 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 16 kDa,37 kDa,36 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28614-578

Isotype

IgG

Carrier free

Yes

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Saccharomyces cerevisiae S288C": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Saccharomyces cerevisiae S288C": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab324844 is the carrier-free version of ab322402.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Not known; suppressor of MIF2 mutations.
See full target information SMT3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com