AB15716

Anti-SMUG1 antibody

Name: Anti-SMUG1 antibody (ab15716) | Abcam
Brand: Abcam Limited
SKU: AB15716
Price: 535 USD
Availability: InStock

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(2 Publications)

Goat Polyclonal SMUG1 antibody. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Single-strand selective monofunctional uracil DNA glycosylase aa 1-50.

View Alternative Names

Single-strand selective monofunctional uracil DNA glycosylase, SMUG1

3 Images

Supplier Data

Immunocytochemistry - Anti-SMUG1 antibody (AB15716)

Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling SMUG1 with ab15716 at 10 μg/mL showing strong nuclear and cytoplasmic staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 μg/mL (green). Nuclear DNA was labelled with DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).

Supplier Data

Immunocytochemistry - Anti-SMUG1 antibody (AB15716)

Immunocytochemistry/immunofluorescence analysis of U2OS cells labelling SMUG1 with ab15716 at 10 μg/mL showing strong nuclear staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 μg/mL (green). Nuclear DNA was labelled with DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).

Flow Cytometry (Intracellular) - Anti-SMUG1 antibody (AB15716)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SMUG1 antibody (AB15716)

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) labelling SMUG1 with ab15716. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (1 μg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor^® 488 secondary antibody.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human Single-strand selective monofunctional uracil DNA glycosylase aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q53HV7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMUG1 also known as Single-strand-selective Monofunctional Uracil-DNA Glycosylase 1 plays a role in DNA repair mechanisms. It is an enzyme involved in the base excision repair (BER) pathway specializing in the removal of uracil from single-stranded DNA. SMUG1 has a molecular weight of about 33 kDa. The protein is expressed in various tissues with notable presence in the brain liver and lung. Its expression levels adjust according to the tissue's need for active DNA repair.

Biological function summary

The process of DNA metabolism relies heavily on mechanisms related to the SMUG1 enzyme. As part of the DNA repair machinery it identifies and excises uracil bases from DNA which result from deamination of cytosine or misincorporation during DNA synthesis. SMUG1 does not function within a larger protein complex but interacts dynamically with other repair proteins like AP endonucleases. This activity helps maintain genomic stability by preventing mutations that could arise from uracil incorporation.

Pathways

SMUG1 contributes importantly to the base excision repair pathway an important process for DNA maintenance. This pathway addresses small non-helix-distorting base lesions such as deaminated oxidized or alkylated bases. Within this pathway SMUG1's activity aligns with other glycosylases and the APEX1 protein which processes the abasic sites left after SMUG1 removes uracil. Furthermore SMUG1's function supports the overall cellular DNA damage response signaling when repair and further processing are necessary.

Alterations in the SMUG1 protein have connections to cancer and neurodegeneration. Loss of SMUG1 activity may result in accumulation of mutations due to unprocessed uracil in the DNA contributing to tumorigenesis. In particular studies have shown links between SMUG1 and glioma where defective DNA repair mechanisms escalate mutation rates. Also in neurodegenerative disorders it contributes directly through genomic instability with connections to APEX1 showing potential overlapping repair functions critical for neuron maintenance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following : ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.

See full target information Single-strand selective monofunctional uracil DNA glycosylase

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 42:1698-710 PubMed24178031

2013

Vpr expression abolishes the capacity of HIV-1 infected cells to repair uracilated DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Eldin,Nathalie Chazal,David Fenard,Eric Bernard,Jean-François Guichou,Laurence Briant

FASEB journal : official publication of the Federa 25:4406-14 PubMed21891782

2011

p73 protein regulates DNA damage repair.

Applications

Species

Human

Elena Zaika,Jinxiong Wei,Dengping Yin,Claudia Andl,Ute Moll,Wael El-Rifai,Alexander I Zaika

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMUG1 antibody

Immunocytochemistry - Anti-SMUG1 antibody (AB15716)

Immunocytochemistry - Anti-SMUG1 antibody (AB15716)

Flow Cytometry (Intracellular) - Anti-SMUG1 antibody (AB15716)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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