AB192240

Anti-SMUG1 antibody [EPR15624]

Name: Anti-SMUG1 antibody [EPR15624] (ab192240) | Abcam
Brand: Abcam Limited
SKU: AB192240
Price: 325 USD
Availability: InStock
Rating: 5 (3 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(3 Reviews)

(10 Publications)

Rabbit Recombinant Monoclonal SMUG1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications.

View Alternative Names

Single-strand selective monofunctional uracil DNA glycosylase, SMUG1

3 Images

Unknown

Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (AB192240)

Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab192240 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (ab192240)

Predicted band size: 29 kDa

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Lab

Western blot - Anti-SMUG1 antibody [EPR15624] (AB192240)

Lanes 1 - 4 : Merged signal (red and green). Green - ab192240 observed at 30 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab192240 was shown to react with SMUG1 in wild-type HAP1 cells in western blot with loss of signal observed in SMUG1 knockout sample. Wild-type and SMUG1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab192240 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

SMUG1 knockout HAP1 cell lysate at 20 µg

Lane 3:

MOLT-4 cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Predicted band size: 29 kDa

Observed band size: 30 kDa

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Supplier Data

Western blot - Anti-SMUG1 antibody [EPR15624] (AB192240)

All lanes:

Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/5000 dilution

Lane 1:

MOLT4 cell lysate at 10 µg

Lane 2:

293 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 29 kDa

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805 Alexa Fluor® 790

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed
HRP

Goat Anti-Rabbit IgG H&L (HRP)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15624

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMUG1 also known as Single-strand-selective Monofunctional Uracil-DNA Glycosylase 1 plays a role in DNA repair mechanisms. It is an enzyme involved in the base excision repair (BER) pathway specializing in the removal of uracil from single-stranded DNA. SMUG1 has a molecular weight of about 33 kDa. The protein is expressed in various tissues with notable presence in the brain liver and lung. Its expression levels adjust according to the tissue's need for active DNA repair.

Biological function summary

The process of DNA metabolism relies heavily on mechanisms related to the SMUG1 enzyme. As part of the DNA repair machinery it identifies and excises uracil bases from DNA which result from deamination of cytosine or misincorporation during DNA synthesis. SMUG1 does not function within a larger protein complex but interacts dynamically with other repair proteins like AP endonucleases. This activity helps maintain genomic stability by preventing mutations that could arise from uracil incorporation.

Pathways

SMUG1 contributes importantly to the base excision repair pathway an important process for DNA maintenance. This pathway addresses small non-helix-distorting base lesions such as deaminated oxidized or alkylated bases. Within this pathway SMUG1's activity aligns with other glycosylases and the APEX1 protein which processes the abasic sites left after SMUG1 removes uracil. Furthermore SMUG1's function supports the overall cellular DNA damage response signaling when repair and further processing are necessary.

Alterations in the SMUG1 protein have connections to cancer and neurodegeneration. Loss of SMUG1 activity may result in accumulation of mutations due to unprocessed uracil in the DNA contributing to tumorigenesis. In particular studies have shown links between SMUG1 and glioma where defective DNA repair mechanisms escalate mutation rates. Also in neurodegenerative disorders it contributes directly through genomic instability with connections to APEX1 showing potential overlapping repair functions critical for neuron maintenance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following : ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.

See full target information Single-strand selective monofunctional uracil DNA glycosylase

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26617 PubMed40695981

2025

A prognostic signature for hormone receptor-positive, human epidermal growth factor receptor 2-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yincheng Liu,Ningyi Xue,Feidie Duan,Kunli Zhao,Yan Liang,Jing Zhao,Lei Zhang,Yu Xu,Yuzi Zhang,Guoqiang Wang,Shangli Cai,Tianyu Zeng,Shui Wang

Molecular cell 84:2036-2052.e7 PubMed38688279

2024

Unprocessed genomic uracil as a source of DNA replication stress in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sneha Saxena,Christopher S Nabel,Turner W Seay,Parasvi S Patel,Ajinkya S Kawale,Caroline R Crosby,Helene Tigro,Eugene Oh,Matthew G Vander Heiden,Aaron N Hata,Zucai Suo,Lee Zou

International journal of biological sciences 20:1669-1687 PubMed38481813

2024

Tubule-specific cyclin-dependent kinase 12 knockdown potentiates kidney injury through transcriptional elongation defects.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Lin Zhang,Tao-Tao Tang,Wei-Jie Ni,Zhong-Tang Li,Liang-Yun-Zi Jiang,Yao Wang,Xuan Zhou,Jing-Yuan Cao,Qing Yin,Wei Jiang,Ya-Jie Zhao,Wei-Hua Gan,Ai-Qing Zhang,Zuo-Lin Li,Yi Wen,Lin-Li Lv,Bi-Cheng Liu,Bin Wang

Molecular cell 84:447-462.e10 PubMed38244544

2024

BRCA2 promotes genomic integrity and therapy resistance primarily through its role in homology-directed repair.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Xin Lim,Mahdia Zaman,Weiran Feng,Maria Jasin

Nucleic acids research 51:4881-4898 PubMed36971122

2023

UV-DDB stimulates the activity of SMUG1 during base excision repair of 5-hydroxymethyl-2'-deoxyuridine moieties.

Applications

Unspecified application

Species

Unspecified reactive species

Sunbok Jang,Sripriya J Raja,Vera Roginskaya,Matthew A Schaich,Simon C Watkins,Bennett Van Houten

Frontiers in cardiovascular medicine 9:1053697 PubMed36620624

2022

5mC modification patterns provide novel direction for early acute myocardial infarction detection and personalized therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqun Guo,Hua Jiang,Jinlong Wang,Ping Li,Xiaoquan Zeng,Tao Zhang,Jianyi Feng,Ruqiong Nie,Yulong Liu,Xiaobian Dong,Qingsong Hu

Nature 607:799-807 PubMed35859169

2022

Mechanisms of APOBEC3 mutagenesis in human cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mia Petljak,Alexandra Dananberg,Kevan Chu,Erik N Bergstrom,Josefine Striepen,Patrick von Morgen,Yanyang Chen,Hina Shah,Julian E Sale,Ludmil B Alexandrov,Michael R Stratton,John Maciejowski

Nature 606:930-936 PubMed35477155

2022

Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shangming Tang,Ema Stokasimov,Yuxiang Cui,David Pellman

Molecular cell 81:767-783.e11 PubMed33333017

2020

Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD.

Applications

Unspecified application

Species

Unspecified reactive species

Graeme Hewitt,Valerie Borel,Sandra Segura-Bayona,Tohru Takaki,Phil Ruis,Roberto Bellelli,Laura C Lehmann,Lucia Sommerova,Aleksandra Vancevska,Antonia Tomas-Loba,Kang Zhu,Christopher Cooper,Kasper Fugger,Harshil Patel,Robert Goldstone,Deborah Schneider-Luftman,Ellie Herbert,Gordon Stamp,Rachel Brough,Stephen Pettitt,Christopher J Lord,Stephen C West,Ivan Ahel,Dragana Ahel,J Ross Chapman,Sebastian Deindl,Simon J Boulton

Cell reports 28:1690-1702.e10 PubMed31412240

2019

SMUG1 Promotes Telomere Maintenance through Telomerase RNA Processing.

Applications

Unspecified application

Species

Unspecified reactive species

Penelope Kroustallaki,Lisa Lirussi,Sergio Carracedo,Panpan You,Q Ying Esbensen,Alexandra Götz,Laure Jobert,Lene Alsøe,Pål Sætrom,Sarantis Gagos,Hilde Nilsen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMUG1 antibody [EPR15624]

Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (AB192240)

Western blot - Anti-SMUG1 antibody [EPR15624] (AB192240)

Western blot - Anti-SMUG1 antibody [EPR15624] (AB192240)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise