Anti-SMUG1 antibody [EPR15624] - BSA and Azide free

• IP

Unknown

Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (AB251114)

This data was developed using ab192240, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab192240 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (<a href='/en-us/products/primary-antibodies/smug1-antibody-epr15624-ab192240'>ab192240</a>)

Predicted band size: 29 kDa

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• WB

Lab

Western blot - Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (AB251114)

This data was developed using the same antibody clone in a different buffer formulation (ab192240).

Lanes 1 - 4 : Merged signal (red and green). Green - ab192240 observed at 30 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab192240 was shown to react with SMUG1 in wild-type HAP1 cells in western blot with loss of signal observed in SMUG1 knockout sample. ild-type and SMUG1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab192240 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SMUG1 antibody [EPR15624] (<a href='/en-us/products/primary-antibodies/smug1-antibody-epr15624-ab192240'>ab192240</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

SMUG1 knockout HAP1 cell lysate at 20 µg

Lane 3:

MOLT-4 cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Predicted band size: 29 kDa

Observed band size: 30 kDa

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• WB

Supplier Data

Western blot - Anti-SMUG1 antibody [EPR15624] - BSA and Azide free (AB251114)

This data was developed using ab192240, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SMUG1 antibody [EPR15624] (<a href='/en-us/products/primary-antibodies/smug1-antibody-epr15624-ab192240'>ab192240</a>) at 1/5000 dilution

Lane 1:

MOLT4 cell lysate at 10 µg

Lane 2:

293 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 29 kDa

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