Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free

• WB

Lab

Western blot - Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (AB239855)

This data was developed using the same antibody clone in a different buffer formulation (ab53316).

Lanes 1-3 : Merged signal (red and green). Green - ab53316 observed at 86 kDa. Red - loading control ab8245 observed at 36 kDa.

ab53316 Anti-SMURF 2 antibody [EP629Y3] was shown to specifically react with SMURF 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265231 (knockout cell lysate ab257691) was used. Wild-type and SMURF 2 knockout samples were subjected to SDS-PAGE. ab53316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SMURF 2 antibody [EP629Y3] (<a href='/en-us/products/primary-antibodies/smurf-2-antibody-ep629y3-ab53316'>ab53316</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SMURF2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SMURF2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-smurf2-knockout-hela-cell-line-ab265231'>ab265231</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 86 kDa

Observed band size: 86 kDa

false

• WB

Lab

Western blot - Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (AB239855)

This data was developed using the same antibody clone in a different buffer formulation (ab53316).

Lanes 1-3 : Merged signal (red and green). Green - ab53316 observed at 86 kDa. Red - loading control ab8245 observed at 36 kDa.

ab53316 Anti-SMURF 2 antibody [EP629Y3] was shown to specifically react with SMURF 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264806 (knockout cell lysate ab257690) was used. Wild-type and SMURF 2 knockout samples were subjected to SDS-PAGE. ab53316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SMURF 2 antibody [EP629Y3] (<a href='/en-us/products/primary-antibodies/smurf-2-antibody-ep629y3-ab53316'>ab53316</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SMURF2 knockout HeLa cell lysate at 20 µg

Lane 3:

U-2 OS cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 86 kDa

Observed band size: 86 kDa

false