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AB236081

Anti-SMURF1 antibody

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(6 Publications)

Rabbit Polyclonal SMURF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMURF1 aa 150-400.

View Alternative Names

KIAA1625, SMURF1, E3 ubiquitin-protein ligase SMURF1, hSMURF1, HECT-type E3 ubiquitin transferase SMURF1, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for SMURF1 using ab236081 at 1/100 dilution in ICC/IF. An Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for SMURF1 using ab236081 at 1/100 dilution in ICC/IF. An Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMURF1 antibody (AB236081)

Immunofluorescent analysis of 4% formaldehyde fixed, 0.2% Triton X-100 permeabolized A549 (Human lung carcinoma cell line) cells labeling SMURF1 with ab236081 at 1/50 dilution, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody (green). Counterstained with DAPI.

Western blot - Anti-SMURF1 antibody (AB236081)
  • WB

Supplier Data

Western blot - Anti-SMURF1 antibody (AB236081)

All lanes:

Western blot - Anti-SMURF1 antibody (ab236081) at 1/1000 dilution

Lane 1:

U251 whole cell lysate

Lane 2:

A549 whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 86 kDa

Observed band size: 87 kDa

false

Exposure time: 5min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SMURF1 aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q9HCE7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMURF1 also known as SMAD-specific E3 ubiquitin protein ligase 1 functions as an E3 ubiquitin ligase involved in the targeted degradation of proteins. This protein weighs approximately 72 kDa and expresses primarily in various human tissues including bone lung and kidney. SMURF1 plays an essential role in regulating protein turnover by tagging substrates for proteasomal degradation. It interacts with substrates through a C2 domain which aids in membrane localization and substrate recognition effectively controlling cellular protein levels and their functions.
Biological function summary

SMURF1 influences numerous cellular processes such as bone morphogenetic protein (BMP) signaling by regulating the availability and stability of signaling molecules. It does not function in isolation; it is a component of a ubiquitin-proteasome system complex that includes other cofactors assisting its activity. SMURF1 therefore affects cell growth differentiation and homeostasis by managing signal transduction pathways critical for these cellular activities.

Pathways

SMURF1 is involved in the BMP and Wnt signaling pathways. Both are fundamental for various biological activities including embryonic development and cell cycle regulation. Within these pathways SMURF1 influences the activity of SMAD6 and related proteins modulating their turnover and impact on downstream signaling processes. These pathways also interact with other cellular pathways but SMURF1's specific role remains concentrated on modulating protein ubiquitination events.

SMURF1 has been linked to cancer and osteoporosis. Its dysregulation can disrupt normal cellular signaling contributing to tumorigenesis or impaired bone maintenance. In cancer its interaction with proteins such as SMAD7 can alter cell signaling promoting uncontrolled cell growth. In osteoporosis SMURF1's regulation of bone remodeling processes might be compromised leading to decreased bone mass and fragility. Understanding SMURF1's roles may open avenues for therapeutic interventions in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed : 23087404). Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed : 21791611). Plays a role in dendrite formation by melanocytes (PubMed : 23999003).
See full target information SMURF1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:735-751 PubMed37909662

2023

SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Xia,Hanfei Zheng,Yang Li,Wanting Xu,Chengwei Wu,Jiachen Xu,Shanhu Li,Lingqiang Zhang,Lei Dong

Laboratory investigation; a journal of technical methods and pathology 102:1121-1131 PubMed35710596

2022

PAQR3 depletion accelerates diabetic wound healing by promoting angiogenesis through inhibiting STUB1-mediated PPARγ degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Qiu,Chang Shu,Xin Li,Wei-Chang Zhang

Bioengineered 13:2371-2386 PubMed35034538

2022

miR-129-5p restores cardiac function in rats with chronic heart failure by targeting the E3 ubiquitin ligase Smurf1 and promoting PTEN expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Qi,Yan Tang,Lu Yin,Keke Ding,Cuimei Zhao,Wenwen Yan,Yi'an Yao

Aging 13:12239-12257 PubMed33872218

2021

Extracellular-vesicle containing miRNA-503-5p released by macrophages contributes to atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuquan Wang,Zhengmin Xu,Xiaoli Wang,Jiankang Zheng,Lihan Peng,Yunfei Zhou,Yongyan Song,Zhan Lu

British journal of pharmacology 178:2246-2265 PubMed33085791

2021

Regulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signalling via interaction with Gli1.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhang,Feixia Wang,Jianlin He,Naqi Lian,Zhenyi Wang,Jiangjuan Shao,Hai Ding,Shanzhong Tan,Anping Chen,Zili Zhang,Shijun Wang,Shizhong Zheng

Frontiers in medicine 7:577578 PubMed33425934

2020

Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-25 Regulates the Ubiquitination and Degradation of Runx2 by SMURF1 to Promote Fracture Healing in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yikun Jiang,Jun Zhang,Zhengwei Li,Guoliang Jia
View all publications
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