Anti-SMURF1 antibody [EPR25111-116]

• WB

Lab

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Smurf1 is degraded through the ubiquitin–proteasome system(PMID : 20580715).

All lanes:

Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

786-O (Human kidney renal cell adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 6:

Mouse lung tissue lysate at 20 µg

Lane 7:

Mouse liver tissue lysate at 20 µg

Lane 8:

Rat lung tissue lysate at 20 µg

Lane 9:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,87 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

In lane 1, lysate was freshlymade and used for Western Blotting immediately to minimize protein degradation. In lane 2, the lysate was stored at -80℃ prior to Western Blotting.

The blot of lane 2 was developed using a high sensitivity ECL substrate.

Exposure time : Lane 1 : 158 seconds, Lane 2 : 3 minutes

All lanes:

Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 86 kDa

Observed band size: 87 kDa

false

• WB

Supplier Data

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

MCF7 transfected with siRNA specifically targeting Smurf1, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 86 kDa

Observed band size: 87 kDa

true

Exposure time: 3min

• WB

Lab

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Smurf1 is degraded through the ubiquitin–proteasome system(PMID : 20580715).

All lanes:

Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

Lane 1:

Fresh mouse liver tissue lysate at 20 µg

Lane 2:

Frozen mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false

Exposure time: 180s