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Rabbit Recombinant Monoclonal SMURF1 antibody. Suitable for WB and reacts with Rat, Mouse, Human, Transfected cell line samples. Cited in 1 publication.

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Images

Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408), expandable thumbnail
  • Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408), expandable thumbnail
  • Western blot - Anti-SMURF1 antibody [EPR25111-116] (AB300408), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Transfected cell line
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species
Human, Rat, Mouse, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human, Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Transfected cell line
Dilution info
1/1000
Notes

-

Target data

Function

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed:21791611). Plays a role in dendrite formation by melanocytes (PubMed:23999003).

Alternative names

KIAA1625, SMURF1, E3 ubiquitin-protein ligase SMURF1, hSMURF1, HECT-type E3 ubiquitin transferase SMURF1, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1

Recommended products

Rabbit Recombinant Monoclonal SMURF1 antibody. Suitable for WB and reacts with Rat, Mouse, Human, Transfected cell line samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25111-116
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SMURF1 also known as SMAD-specific E3 ubiquitin protein ligase 1 functions as an E3 ubiquitin ligase involved in the targeted degradation of proteins. This protein weighs approximately 72 kDa and expresses primarily in various human tissues including bone lung and kidney. SMURF1 plays an essential role in regulating protein turnover by tagging substrates for proteasomal degradation. It interacts with substrates through a C2 domain which aids in membrane localization and substrate recognition effectively controlling cellular protein levels and their functions.

Biological function summary

SMURF1 influences numerous cellular processes such as bone morphogenetic protein (BMP) signaling by regulating the availability and stability of signaling molecules. It does not function in isolation; it is a component of a ubiquitin-proteasome system complex that includes other cofactors assisting its activity. SMURF1 therefore affects cell growth differentiation and homeostasis by managing signal transduction pathways critical for these cellular activities.

Pathways

SMURF1 is involved in the BMP and Wnt signaling pathways. Both are fundamental for various biological activities including embryonic development and cell cycle regulation. Within these pathways SMURF1 influences the activity of SMAD6 and related proteins modulating their turnover and impact on downstream signaling processes. These pathways also interact with other cellular pathways but SMURF1's specific role remains concentrated on modulating protein ubiquitination events.

Associated diseases and disorders

SMURF1 has been linked to cancer and osteoporosis. Its dysregulation can disrupt normal cellular signaling contributing to tumorigenesis or impaired bone maintenance. In cancer its interaction with proteins such as SMAD7 can alter cell signaling promoting uncontrolled cell growth. In osteoporosis SMURF1's regulation of bone remodeling processes might be compromised leading to decreased bone mass and fragility. Understanding SMURF1's roles may open avenues for therapeutic interventions in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408), expandable thumbnail

    Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408)

    SMURF1 Western blot staining using rabbit Anti-SMURF1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Smurf1 is degraded through the ubiquitin–proteasome system(PMID: 20580715).

    All lanes: Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

    Lane 1: Fresh mouse liver tissue lysate at 20 µg

    Lane 2: Frozen mouse liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 55 kDa

    Exposure time: 180s

  • Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408), expandable thumbnail

    Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408)

    SMURF1 Western blot staining using rabbit Anti-SMURF1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Smurf1 is degraded through the ubiquitin–proteasome system(PMID: 20580715).

    All lanes: Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 786-O (Human kidney renal cell adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 5: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 6: Mouse lung tissue lysate at 20 µg

    Lane 7: Mouse liver tissue lysate at 20 µg

    Lane 8: Rat lung tissue lysate at 20 µg

    Lane 9: Rat liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 55 kDa, 87 kDa

    Exposure time: 180s

  • Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408), expandable thumbnail

    Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-SMURF1 antibody [EPR25111-116] (ab300408) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

    Lane 2: MCF7 transfected with siRNA specifically targeting Smurf1, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 86 kDa

    Observed band size: 87 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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