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AB313470

Anti-SMURF2 antibody [RP23040176]

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(1 Publication)

Rabbit Recombinant Multiclonal SMURF 2 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SMURF2.

View Alternative Names

E3 ubiquitin-protein ligase SMURF2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-SMURF2 Antibody [RP23040176] (AB313470)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMURF2 Antibody [RP23040176] (AB313470)

Immunofluorescence analysis was performed on fixed and permeabilized MDA-MB-231 cells for detection of endogenous Smurf2 using ab313470 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate ( 1 : 2000). Panel a) shows representative cells that were stained for detection and localization of Smurf2 protein (green), Panel b) is stained for nuclei (blue) using with DAPI . Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin ( 1 : 300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Smurf2. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040176

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SMURF2.

Q9HAU4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMURF2 also known as SMAD specific E3 ubiquitin protein ligase 2 functions as an E3 ubiquitin ligase involved in protein ubiquitination. It weighs approximately 85 kDa and is part of the NEDD4 family of E3 ligases. SMURF2 facilitates the transfer of ubiquitin from an E2 conjugating enzyme to specific substrate proteins marking them for degradation via the proteasome pathway. This protein is widely expressed in various tissues including brain kidney and liver indicating its essential role in different cellular processes.
Biological function summary

SMURF2 regulates many cell signaling pathways including the TGF-beta/Smad pathway. It associates with SMAD proteins to control their stability and activity acting as a negative regulator to modulate TGF-beta signaling. By promoting the ubiquitination and degradation of receptor-regulated SMADs (R-SMADs) SMURF2 impacts cellular responses to growth factors. This regulatory mechanism is important for maintaining cellular proliferation differentiation and apoptosis.

Pathways

The function of SMURF2 significantly influences the TGF-beta and BMP signaling pathways both critical in controlling cell growth and differentiation. These pathways involve interactions with other proteins like SMAD7 and SMAD2. SMURF2 interacts with SMAD7 an inhibitory SMAD to regulate Smad-dependent transcriptional responses ensuring the fine-tuning of signal transduction. These pathways' balance and regulation are vital for appropriate cellular function and response.

SMURF2 has connections to cancer and fibrosis. Aberrant regulation of SMURF2 contributes to tumorigenesis where its altered expression levels can lead to unrestrained cell growth and cancer progression particularly in breast and prostate cancers. Additionally SMURF2 is involved in fibrosis with its dysregulation affecting extracellular matrix deposition. In both cancer and fibrosis SMURF2 interacts with TGF-beta and other related proteins highlighting its involvement in disease progression and sustaining pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 11016919, PubMed : 38016474). Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling (PubMed : 11163210, PubMed : 12717440, PubMed : 21791611). In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1 (PubMed : 18448069). Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation (PubMed : 11016919, PubMed : 11158580, PubMed : 11389444). SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation (PubMed : 11389444). Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation (PubMed : 30696809). Acts as an activator of ferroptosis by mediating ubiquitination and degradation of GSTP1, thereby preventing detoxification of 4-hydroxynonenal (4-HNE) reactive aldehyde (PubMed : 38016474).. (Microbial infection) In case of filoviruses Ebola/EBOV and Marburg/MARV infection, the complex formed by viral matrix protein VP40 and SMURF2 facilitates virus budding.
See full target information SMURF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 49:798-809 PubMed40244332

2025

IL-17C-Mediated Upregulation of SMURF2 Induces Psoriatic Changes in Keratinocytes by Facilitating PPP6C Ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Mao,Xin Ma,Yuanyuan Sun,Wuqing Wang,Bin Li
View all publications
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Product promise

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