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AB185969

Anti-SMYD1 antibody [EPR13573]

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(1 Publication)

Rabbit Recombinant Monoclonal SMYD1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Histone-lysine N-methyltransferase SMYD1, SET and MYND domain-containing protein 1, SMYD1

2 Images
Western blot - Anti-SMYD1 antibody [EPR13573] (AB185969)
  • WB

Supplier Data

Western blot - Anti-SMYD1 antibody [EPR13573] (AB185969)

All lanes:

Western blot - Anti-SMYD1 antibody [EPR13573] (ab185969) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa

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Western blot - Anti-SMYD1 antibody [EPR13573] (AB185969)
  • WB

Supplier Data

Western blot - Anti-SMYD1 antibody [EPR13573] (AB185969)

All lanes:

Western blot - Anti-SMYD1 antibody [EPR13573] (ab185969) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13573

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SET and MYND domain-containing protein 1 (SMYD1) also known as BOP is a protein of approximately 56 kDa that functions as a histone methyltransferase. It modifies histones by transferring methyl groups to specific lysine residues particularly histone H3 at lysine 4 (H3K4). SMYD1 expression is mainly observed in cardiac and skeletal muscle tissues. It plays a role in regulating gene expression by altering chromatin structure therefore influencing transcriptional activity.
Biological function summary

SMYD1 plays a significant role in muscle differentiation and development. Its activity leads to chromatin modifications that facilitate muscle-specific gene expression. SMYD1 interacts in a complex network with other proteins involved in muscle function acting as part of the transcriptional machinery that marks certain genes for activation. This methyltransferase activity contributes to the proper formation of muscle fibers.

Pathways

SMYD1 is important in pathways that control heart development and muscle function. It is active in the MAPK signaling pathway which is essential for cell growth and differentiation and interacts with proteins such as MEF2C a transcription factor important for muscle development. This connection influences the regulation and expression of various genes that are critical for normal cardiac and skeletal muscle function.

Mutations or dysregulation of SMYD1 can lead to congenital heart defects and myopathies. Its interactions with proteins like MYH7 a myosin heavy chain associated with proper cardiac muscle function are important in these conditions. Anomalies in SMYD1 activity can disrupt normal cardiac and skeletal muscle development leading to diseases characterized by structural or functional abnormalities of the heart and muscles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
See full target information SMYD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biomedicine 5:31 PubMed39117956

2024

Inhibition of Sesn2 has negative regulatory effects on the myogenic differentiation of C2C12 myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zubiao Song,Qing Lin,Jiahui Liang,Weixi Zhang
View all publications
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Product promise

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