AB250496

Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SMYD1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

View Alternative Names

Histone-lysine N-methyltransferase SMYD1, SET and MYND domain-containing protein 1, SMYD1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free (AB250496)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free (AB250496)

This data was developed using ab181372, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SMYD1 with ab181372 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free (AB250496)

This data was developed using ab181372, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SMYD1 with ab181372 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free (AB250496)

This data was developed using ab181372, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SMYD1 antibody [EPR13574(B)-30] (<a href='/en-us/products/primary-antibodies/smyd1-antibody-epr13574b-30-ab181372'>ab181372</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human skeletal muscle tissue lysate at 10 µg

Predicted band size: 56 kDa,60 kDa

Observed band size: 60 kDa

false

Supplier Data

Immunoprecipitation - Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free (AB250496)

This data was developed using ab181372, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal heart lysate or (Lane 2) 1XPBS (negative control) immunoprecipitated using ab181372 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-SMYD1 antibody [EPR13574(B)-30] (<a href='/en-us/products/primary-antibodies/smyd1-antibody-epr13574b-30-ab181372'>ab181372</a>)

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13574(B)-30

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab250496 is the carrier-free version of ab181372.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SET and MYND domain-containing protein 1 (SMYD1) also known as BOP is a protein of approximately 56 kDa that functions as a histone methyltransferase. It modifies histones by transferring methyl groups to specific lysine residues particularly histone H3 at lysine 4 (H3K4). SMYD1 expression is mainly observed in cardiac and skeletal muscle tissues. It plays a role in regulating gene expression by altering chromatin structure therefore influencing transcriptional activity.

Biological function summary

SMYD1 plays a significant role in muscle differentiation and development. Its activity leads to chromatin modifications that facilitate muscle-specific gene expression. SMYD1 interacts in a complex network with other proteins involved in muscle function acting as part of the transcriptional machinery that marks certain genes for activation. This methyltransferase activity contributes to the proper formation of muscle fibers.

Pathways

SMYD1 is important in pathways that control heart development and muscle function. It is active in the MAPK signaling pathway which is essential for cell growth and differentiation and interacts with proteins such as MEF2C a transcription factor important for muscle development. This connection influences the regulation and expression of various genes that are critical for normal cardiac and skeletal muscle function.

Mutations or dysregulation of SMYD1 can lead to congenital heart defects and myopathies. Its interactions with proteins like MYH7 a myosin heavy chain associated with proper cardiac muscle function are important in these conditions. Anomalies in SMYD1 activity can disrupt normal cardiac and skeletal muscle development leading to diseases characterized by structural or functional abnormalities of the heart and muscles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.

See full target information SMYD1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free (AB250496)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free (AB250496)

Western blot - Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free (AB250496)

Immunoprecipitation - Anti-SMYD1 antibody [EPR13574(B)-30] - BSA and Azide free (AB250496)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise