AB155018

Anti-SMYD3 antibody

Name: Anti-SMYD3 antibody (ab155018) | Abcam
Brand: Abcam Limited
SKU: AB155018
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SMYD3 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMYD3 aa 1 to C-terminus.

View Alternative Names

ZMYND1, ZNFN3A1, SMYD3, Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1

3 Images

Supplier Data

Western blot - Anti-SMYD3 antibody (AB155018)

10% SDS-PAGE

All lanes:

Western blot - Anti-SMYD3 antibody (ab155018) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

SK-OV-3 (human ovarian cancer cell line) whole cell lysate at 30 µg

Lane 3:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-SMYD3 antibody (AB155018)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-SMYD3 antibody (ab155018) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-SMYD3 antibody (AB155018)

10% SDS-PAGE

All lanes:

Western blot - Anti-SMYD3 antibody (ab155018)

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

Transfected HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human SMYD3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H7B4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMYD3 short for SET and MYND domain containing 3 is a member of the protein lysine methyltransferase family. It mainly functions as a histone methyltransferase adding methyl groups to lysine 4 on histone H3 (H3K4) and lysine 5 on histone H4 (H4K5) influencing chromatin structure and gene expression. SMYD3 has a molecular mass of approximately 56 kDa. It is widely expressed in multiple tissues but levels are higher in skeletal muscle and the heart. This broad expression indicates its potential role in various cellular functions.

Biological function summary

This methyltransferase regulates transcriptional activation by methylating histones. It acts as part of transcriptional coactivator complexes interacting with RNA polymerase II. SMYD3 modulates the expression of genes involved in cell proliferation and cancer progression. Additionally it interacts with proteins like Heat Shock Protein 90 (HSP90) and promotes the stabilization of substrate proteins. Its influence extends to non-histone proteins which suggests a wider impact on cellular signaling and structure.

Pathways

SMYD3 is involved in the MAPK (Mitogen-Activated Protein Kinase) signaling pathway an important regulator of cell differentiation proliferation and survival. It interacts with key proteins in this pathway such as ERK2 (Extracellular signal-regulated kinase 2) influencing cellular outcomes. SMYD3 also plays a role in the Wnt signaling pathway which is important for development and oncogenesis. This interaction with signaling pathways underlines its significant regulatory functions within the cell.

SMYD3 is strongly associated with cancer. Its overexpression contributes to tumorigenesis and metastasis particularly in colorectal and liver cancers. In cancer SMYD3 interacts with proteins like AKT1 promoting cancer cell survival and growth. Additionally SMYD3 has been linked to congenital heart diseases through its role in cardiac development. It interacts with cardiac transcription factors further connecting its function to heart malformations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed : 15235609, PubMed : 22419068). Also methylates 'Lys-5' of histone H4 (PubMed : 22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed : 15235609). Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed : 15235609).

See full target information SMYD3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 28:1 PubMed34979900

2022

Ketogenic diet ameliorates lipid dysregulation in type 2 diabetic mice by downregulating hepatic pescadillo 1.

Applications

Unspecified application

Species

Unspecified reactive species

Jielin Zhou,Yao Lu,Yajing Jia,Jing Lu,Zhengxuan Jiang,Keyang Chen

BMC pulmonary medicine 17:24 PubMed28137284

2017

Epigenetic modifying enzyme expression in asthmatic airway epithelial cells and fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Dorota Stefanowicz,Jari Ullah,Kevin Lee,Furquan Shaheen,Ekiomoado Olumese,Nick Fishbane,Hyun-Kyoung Koo,Teal S Hallstrand,Darryl A Knight,Tillie-Louise Hackett

View all publications

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