Anti-SMYD3 Antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 Antibody (AB327239)

Immunohistochemical staining of human testis using ab327239 at 1 : 500–1 : 1000 dilution, showing moderate cytoplasmic positivity in cells in seminiferous ducts, Leydig cells were strongly stained.

• ChIP

Supplier Data

ChIP - Anti-SMYD3 Antibody (AB327239)

ChIP-Exo-Seq composite graph for ab327239 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-SMYD3 Antibody (AB327239)

Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue using ab327239 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-SMYD3 Antibody (ab327239)

Lane 2:

Human cell line RT-4

Lane 3:

Human cell line U-251 MG

Lane 4:

Human plasma

Lane 5:

Human liver tissue

Lane 6:

Human tonsil tissue

Predicted band size: 49.1 kDa

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