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Rabbit Recombinant Monoclonal SMYD3 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 15 publications.

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Images

Western blot - Anti-SMYD3 antibody [EPR11107(2)] (AB187149), expandable thumbnail
  • Western blot - Anti-SMYD3 antibody [EPR11107(2)] (AB187149), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] (AB187149), expandable thumbnail
  • Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (AB187149), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (AB187149), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Expected
Rat
Expected
Expected
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/40 - 1/60
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/20000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/20000
Notes

-

Species
Human
Dilution info
1/1000 - 1/20000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068). Also methylates 'Lys-5' of histone H4 (PubMed:22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609). Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609).

Alternative names

ZMYND1, ZNFN3A1, SMYD3, Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1

Recommended products

Rabbit Recombinant Monoclonal SMYD3 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 15 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR11107(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SMYD3 short for SET and MYND domain containing 3 is a member of the protein lysine methyltransferase family. It mainly functions as a histone methyltransferase adding methyl groups to lysine 4 on histone H3 (H3K4) and lysine 5 on histone H4 (H4K5) influencing chromatin structure and gene expression. SMYD3 has a molecular mass of approximately 56 kDa. It is widely expressed in multiple tissues but levels are higher in skeletal muscle and the heart. This broad expression indicates its potential role in various cellular functions.

Biological function summary

This methyltransferase regulates transcriptional activation by methylating histones. It acts as part of transcriptional coactivator complexes interacting with RNA polymerase II. SMYD3 modulates the expression of genes involved in cell proliferation and cancer progression. Additionally it interacts with proteins like Heat Shock Protein 90 (HSP90) and promotes the stabilization of substrate proteins. Its influence extends to non-histone proteins which suggests a wider impact on cellular signaling and structure.

Pathways

SMYD3 is involved in the MAPK (Mitogen-Activated Protein Kinase) signaling pathway an important regulator of cell differentiation proliferation and survival. It interacts with key proteins in this pathway such as ERK2 (Extracellular signal-regulated kinase 2) influencing cellular outcomes. SMYD3 also plays a role in the Wnt signaling pathway which is important for development and oncogenesis. This interaction with signaling pathways underlines its significant regulatory functions within the cell.

Associated diseases and disorders

SMYD3 is strongly associated with cancer. Its overexpression contributes to tumorigenesis and metastasis particularly in colorectal and liver cancers. In cancer SMYD3 interacts with proteins like AKT1 promoting cancer cell survival and growth. Additionally SMYD3 has been linked to congenital heart diseases through its role in cardiac development. It interacts with cardiac transcription factors further connecting its function to heart malformations.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149), expandable thumbnail

    Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    Lanes 1 - 4: Merged signal (red and green). Green - ab187149 observed at 49 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab187149 was shown to react with SMYD3 in HAP1 wild-type cells in Western blot. Loss of signal was observed when SMYD3 knockout sample was used. HAP1 wild-type and SMYD3 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab187149 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: SMYD3 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: MCF7 whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 49 kDa

  • Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149), expandable thumbnail

    Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/20000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma cell line) cell lysate at 20 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

    Lane 3: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 20 µg

    Lane 4: T47-D (human ductal breast epithelial tumor cell line) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] (ab187149), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells, labeling SMYD3 with ab187149 at 1/250 dilution (left image; green); secondary antibody was Goat anti rabbit IgG Alexa Fluor®488, and counterstained with Dapi (right image; blue).

  • Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (ab187149), expandable thumbnail

    Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    Immunoprecipitation analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate labeling SMYD3 using ab187149 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of HeLa lysate.

    All lanes: Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    Intracellular flow cytometric analysis of 2%paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SMYD3 with ab187149 at 1/30 dilution. Secondary antibody wasGoat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control was a Rabbit monoclonal IgG.

  • Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149), expandable thumbnail

    Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/5000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Rat spleen tissue lysate at 10 µg

    Lane 3: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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