AB187149

Anti-SMYD3 antibody [EPR11107(2)]

RabMAb
Recombinant
KO Validated
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(25 Publications)

Rabbit Recombinant Monoclonal SMYD3 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 25 publications.

View Alternative Names

ZMYND1, ZNFN3A1, SMYD3, Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

Intracellular flow cytometric analysis of 2%paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SMYD3 with ab187149 at 1/30 dilution. Secondary antibody wasGoat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control was a Rabbit monoclonal IgG.

Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells, labeling SMYD3 with ab187149 at 1/250 dilution (left image; green); secondary antibody was Goat anti rabbit IgG Alexa Fluor^®488, and counterstained with Dapi (right image; blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat spleen tissue lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

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Supplier Data

Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

Immunoprecipitation analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate labeling SMYD3 using ab187149 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of HeLa lysate.

All lanes:

Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

Predicted band size: 49 kDa

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Lab

Western blot - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

Lanes 1 - 4 : Merged signal (red and green). Green - ab187149 observed at 49 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab187149 was shown to react with SMYD3 in HAP1 wild-type cells in Western blot. Loss of signal was observed when SMYD3 knockout sample was used. HAP1 wild-type and SMYD3 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab187149 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

SMYD3 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

MCF7 whole cell lysate at 20 µg

Predicted band size: 49 kDa

Observed band size: 49 kDa

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Supplier Data

Western blot - Anti-SMYD3 antibody [EPR11107(2)] (AB187149)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/20000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Lane 3:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 20 µg

Lane 4:

T47-D (human ductal breast epithelial tumor cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11107(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMYD3 short for SET and MYND domain containing 3 is a member of the protein lysine methyltransferase family. It mainly functions as a histone methyltransferase adding methyl groups to lysine 4 on histone H3 (H3K4) and lysine 5 on histone H4 (H4K5) influencing chromatin structure and gene expression. SMYD3 has a molecular mass of approximately 56 kDa. It is widely expressed in multiple tissues but levels are higher in skeletal muscle and the heart. This broad expression indicates its potential role in various cellular functions.

Biological function summary

This methyltransferase regulates transcriptional activation by methylating histones. It acts as part of transcriptional coactivator complexes interacting with RNA polymerase II. SMYD3 modulates the expression of genes involved in cell proliferation and cancer progression. Additionally it interacts with proteins like Heat Shock Protein 90 (HSP90) and promotes the stabilization of substrate proteins. Its influence extends to non-histone proteins which suggests a wider impact on cellular signaling and structure.

Pathways

SMYD3 is involved in the MAPK (Mitogen-Activated Protein Kinase) signaling pathway an important regulator of cell differentiation proliferation and survival. It interacts with key proteins in this pathway such as ERK2 (Extracellular signal-regulated kinase 2) influencing cellular outcomes. SMYD3 also plays a role in the Wnt signaling pathway which is important for development and oncogenesis. This interaction with signaling pathways underlines its significant regulatory functions within the cell.

SMYD3 is strongly associated with cancer. Its overexpression contributes to tumorigenesis and metastasis particularly in colorectal and liver cancers. In cancer SMYD3 interacts with proteins like AKT1 promoting cancer cell survival and growth. Additionally SMYD3 has been linked to congenital heart diseases through its role in cardiac development. It interacts with cardiac transcription factors further connecting its function to heart malformations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed : 15235609, PubMed : 22419068). Also methylates 'Lys-5' of histone H4 (PubMed : 22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed : 15235609). Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed : 15235609).

See full target information SMYD3

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:24802 PubMed40634549

2025

Small molecule compound ZYZ-384 targets SMYD3 to alleviate aging.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Dan Han,Li Jin,Xuena Xie,Huibo Li,Qian Ding,Yizhun Zhu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e04200 PubMed40548645

2025

SMYD3 Promotes Immune Evasion in Clear Cell Renal Cell Carcinoma via SREBP1-Mediated Transactivation of CD47.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengfang Liu,Xiumei Zhao,Maolin Zang,Huiyang Yuan,Xin Qin,Xiaofeng Li,Shuo Zhao,Ruirong Tan,Keqiang Yan,Li Liu,Yidong Fan,Ning Zhang,Benkang Shi,Bo Han,Shouzhen Chen

The Journal of biological chemistry 301:110320 PubMed40451434

2025

The transcription factor HOXA9 induces expression of the chromatin modifier SMYD3 to drive leukemogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Zhang,Jinqiu Zhong,Luo Yang,Qianyun Ye,Yanli Jin,Chang Liu,Peilong Lai,Hongle Li,Jingxuan Pan,Bei Jin

Scientific reports 15:984 PubMed39762343

2025

SMYD3 drives cell cycle and epithelial-mesenchymal transition pathways through dual gene transcriptional repression and activation in HPV-negative head and neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Madhavi Murali,Abbas Saeed,Sohyoung Kim,Kyunghee Burkitt,Hui Cheng,Arfa Moshiri,Jawad Akhtar,Daniel Tsai,Marie Luff,Baktiar Karim,Vassiliki Saloura

iScience 27:110854 PubMed39310755

2024

Smyd3-mediated immuno-modulation in HPV-negative head and neck squamous cell carcinoma mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel E Tsai,Alexei Lovanov,Abdalla Abdelmaksoud,Jawad Akhtar,Mohd Saleem Dar,Marie Luff,Katherine McKinnon,Sohyoung Kim,Yvette Robbins,Angel Huynh,Madhavi Murali,Benjamin Bernard,Andrew Sinkoe,Xiaolin Luo,Karim B,Clint T Allen,Vassiliki Saloura

Biological trace element research 203:2013-2028 PubMed39106008

2024

Knockdown of SMYD3 by RNA Interference Regulates the Expression of Autophagy-Related Proteins and Inhibits Bone Formation in Fluoride-Exposed Osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Deng,Xiaoxiao Zeng,Kailin Zhang,Ting Zhang,Yangting Dong,Jian Zou,Changxue Wu,Yi Li,Fucheng Li,Zhizhong Guan

Cell reports 42:112823 PubMed37463106

2023

SMYD3 represses tumor-intrinsic interferon response in HPV-negative squamous cell carcinoma of the head and neck.

Applications

Unspecified application

Species

Unspecified reactive species

Nupur Nigam,Benjamin Bernard,Samantha Sevilla,Sohyoung Kim,Mohd Saleem Dar,Daniel Tsai,Yvette Robbins,Kyunghee Burkitt,Cem Sievers,Clint T Allen,Richard L Bennett,Theophilus T Tettey,Benjamin Carter,Lorenzo Rinaldi,Mark W Lingen,Houssein Sater,Elijah F Edmondson,Arfa Moshiri,Abbas Saeed,Hui Cheng,Xiaolin Luo,Kevin Brennan,Vishal Koparde,Chen Chen,Sudipto Das,Thorkell Andresson,Abdalla Abdelmaksoud,Madhavi Murali,Seiji Sakata,Kengo Takeuchi,Raj Chari,Yusuke Nakamura,Ravindra Uppaluri,John B Sunwoo,Carter Van Waes,Jonathan D Licht,Gordon L Hager,Vassiliki Saloura

Cell death & disease 14:386 PubMed37386026

2023

SMYD3 drives the proliferation in gastric cancer cells via reducing EMP1 expression in an H4K20me3-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zeng,Gang Ma,Fenglin Cai,Pengliang Wang,Han Liang,Rupeng Zhang,Jingyu Deng,Yong Liu

Clinical epigenetics 15:92 PubMed37237385

2023

Histone lysine methyltransferase SMYD3 promotes oral squamous cell carcinoma tumorigenesis via H3K4me3-mediated HMGA2 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Zongcheng Yang,Fen Liu,Zongkai Li,Nianping Liu,Xinfeng Yao,Yu Zhou,Liyu Zhang,Pan Jiang,Honghong Liu,Lingming Kong,Chuandong Lang,Xin Xu,Jihui Jia,Takahito Nakajima,Wenchao Gu,Lixin Zheng,Zhihong Zhang

BMC biology 20:294 PubMed36575438

2022

SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Rongfang Qiu,Siyu Zhao,Lin Shen,Bufu Tang,Qiaoyou Weng,Ziwei Xu,Liyun Zheng,Weiqian Chen,Gaofeng Shu,Yajie Wang,Zhongwei Zhao,Minjiang Chen,Jiansong Ji

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