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Rabbit Recombinant Monoclonal SMYD3 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (AB232626), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (AB232626), expandable thumbnail
  • Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (AB232626), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (AB232626), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (AB232626), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Expected
Rat
Expected
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

5 products for Alternative Version

Target data

Function

Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068). Also methylates 'Lys-5' of histone H4 (PubMed:22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609). Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609).

Alternative names

ZMYND1, ZNFN3A1, SMYD3, Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1

Recommended products

Rabbit Recombinant Monoclonal SMYD3 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR11107(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab232626 is the carrier-free version of Anti-SMYD3 antibody [EPR11107(2)] ab187149.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SMYD3 short for SET and MYND domain containing 3 is a member of the protein lysine methyltransferase family. It mainly functions as a histone methyltransferase adding methyl groups to lysine 4 on histone H3 (H3K4) and lysine 5 on histone H4 (H4K5) influencing chromatin structure and gene expression. SMYD3 has a molecular mass of approximately 56 kDa. It is widely expressed in multiple tissues but levels are higher in skeletal muscle and the heart. This broad expression indicates its potential role in various cellular functions.

Biological function summary

This methyltransferase regulates transcriptional activation by methylating histones. It acts as part of transcriptional coactivator complexes interacting with RNA polymerase II. SMYD3 modulates the expression of genes involved in cell proliferation and cancer progression. Additionally it interacts with proteins like Heat Shock Protein 90 (HSP90) and promotes the stabilization of substrate proteins. Its influence extends to non-histone proteins which suggests a wider impact on cellular signaling and structure.

Pathways

SMYD3 is involved in the MAPK (Mitogen-Activated Protein Kinase) signaling pathway an important regulator of cell differentiation proliferation and survival. It interacts with key proteins in this pathway such as ERK2 (Extracellular signal-regulated kinase 2) influencing cellular outcomes. SMYD3 also plays a role in the Wnt signaling pathway which is important for development and oncogenesis. This interaction with signaling pathways underlines its significant regulatory functions within the cell.

Associated diseases and disorders

SMYD3 is strongly associated with cancer. Its overexpression contributes to tumorigenesis and metastasis particularly in colorectal and liver cancers. In cancer SMYD3 interacts with proteins like AKT1 promoting cancer cell survival and growth. Additionally SMYD3 has been linked to congenital heart diseases through its role in cardiac development. It interacts with cardiac transcription factors further connecting its function to heart malformations.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626), expandable thumbnail

    Western blot - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SMYD3 antibody [EPR11107(2)] ab187149).

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-SMYD3 antibody [EPR11107(2)] ab187149 observed at 49 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    Anti-SMYD3 antibody [EPR11107(2)] ab187149 was shown to react with SMYD3 in HAP1 wild-type cells in Western blot. Loss of signal was observed when SMYD3 knockout sample was used. HAP1 wild-type and SMYD3 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with Anti-SMYD3 antibody [EPR11107(2)] ab187149 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SMYD3 antibody [EPR11107(2)] (Anti-SMYD3 antibody [EPR11107(2)] ab187149) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: SMYD3 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: MCF7 whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells, labeling SMYD3 with Anti-SMYD3 antibody [EPR11107(2)] ab187149 at 1/250 dilution (left image; green); secondary antibody was Goat anti rabbit IgG Alexa Fluor®488, and counterstained with Dapi (right image; blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SMYD3 antibody [EPR11107(2)] ab187149).

  • Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626), expandable thumbnail

    Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunoprecipitation analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate labeling SMYD3 using Anti-SMYD3 antibody [EPR11107(2)] ab187149 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of HeLa lysate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SMYD3 antibody [EPR11107(2)] ab187149).

    All lanes: Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (Anti-SMYD3 antibody [EPR11107(2)] ab187149)

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue, labeling SMYD3 with Anti-SMYD3 antibody [EPR11107(2)] ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SMYD3 antibody [EPR11107(2)] ab187149).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunohistochemical analysis of 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SMYD3 with Anti-SMYD3 antibody [EPR11107(2)] ab187149 at 1/30 dilution. Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control was a Rabbit monoclonal IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SMYD3 antibody [EPR11107(2)] ab187149).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] - BSA and Azide free (ab232626)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue, labeling SMYD3 with Anti-SMYD3 antibody [EPR11107(2)] ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SMYD3 antibody [EPR11107(2)] ab187149).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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