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Rabbit Polyclonal SMYD5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SMYD5 aa 300 to C-terminus.

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Images

Western blot - Anti-SMYD5 antibody - C-terminal (AB137622), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD5 antibody - C-terminal (AB137622), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human SMYD5 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6GMV2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow

Dilution info

-

Notes

-

Target data

Function

Protein-lysine N-trimethyltransferase that specifically catalyzes trimethylation of 'Lys-22' of the RPL40/eL40 subunit of the 60S ribosome, thereby promoting translation elongation and protein synthesis (PubMed:39048817, PubMed:39103523). May also act as a histone methyltransferase in the context of histone octamers, but not on nucleosome substrates: trimethylates 'Lys-36' of histone H3 and 'Lys-20' of histone H4 to form H3K36me3 and H4K20me3, respectively (By similarity). The histone methyltransferase activity, which is independent of its SET domain, is however unsure in vivo (PubMed:39048817, PubMed:39103523). In association with the NCoR corepressor complex, involved in the repression of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages, possibly by catalyzing the formation of H4K20me3 at the gene promoters (By similarity). Plays an important role in embryonic stem (ES) cell self-renewal and differentiation (By similarity). Maintains genome stability of ES cells during differentiation through regulation of heterochromatin formation and repression of endogenous repetitive DNA elements by promoting H4K20me3 marks (PubMed:28951459). Acts as a regulator of the hypothermia response: its degradation in response to mild hypothermia relieves the formation of H3K36me3 at gene promoters, allowing expression of the neuroprotective gene SP1 (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal SMYD5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SMYD5 aa 300 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human SMYD5 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6GMV2
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SMYD5 also known as SET and MYND domain-containing protein 5 functions as a histone methyltransferase and plays a role in regulating gene expression. It typically adds methyl groups to lysine residues on histone proteins which influences chromatin structure and gene transcription. SMYD5 has a mass of around 58 kDa and is found in various tissues with significant expression in the heart skeletal muscle and testis. Its specific localization to these tissues highlights its potential importance in the regulation of gene expression patterns that are tissue-specific.

Biological function summary

SMYD5 engages in modifying chromatin architecture which impacts cellular processes like proliferation and differentiation. It forms part of larger complexes that work in coordination to modify histones including the modification of H4K20 an important target of SMYD5's methyltransferase activity. This modification is linked to the regulation of DNA damage response and cell cycle progression. As a component of these complexes SMYD5 interacts with other proteins to execute its function.

Pathways

Research identifies SMYD5 as an important regulator in the NF-κB signaling pathway a pathway pivotal for immune response and inflammation. By influencing this pathway SMYD5 affects cellular reactions to stress and immune signals. It is known to interact with TAK1 a kinase involved in activating NF-κB demonstrating a connection with inflammatory regulation. Additionally SMYD5 has roles in the epigenetic landscape participating in pathways involving SET domain proteins responsible for chromatin remodeling.

Associated diseases and disorders

SMYD5 has been linked to inflammatory and cancer-related conditions. The regulation of the NF-κB pathway by SMYD5 implicates it in inflammation-related diseases including rheumatoid arthritis where NF-κB activity correlates with inflammatory responses. Furthermore alterations in SMYD5 expression or function might influence tumorigenesis. In these contexts proteins like TAK1 become important as they connect SMYD5 function to key pathways driving disease development. Understanding SMYD5's role offers potential avenues for therapeutic intervention in related conditions.

Product promise

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2 product images

  • Western blot - Anti-SMYD5 antibody - C-terminal (ab137622), expandable thumbnail

    Western blot - Anti-SMYD5 antibody - C-terminal (ab137622)

    10% SDS PAGE

    All lanes: Western blot - Anti-SMYD5 antibody - C-terminal (ab137622) at 1/1000 dilution

    Lane 1: 293T whole cell lysate at 30 µg

    Lane 2: A431 whole cell lysate at 30 µg

    Lane 3: H1299 whole cell lysate at 30 µg

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD5 antibody - C-terminal (ab137622), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD5 antibody - C-terminal (ab137622)

    ab137622 at a 1/100 dilution, staining SMYD5 in paraffin embedded NCI-N87 xenograft tissue by Immunohistochemistry.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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