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AB317193

Anti-SNAI1 antibody [HL2303]

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Rabbit Monoclonal SNAIL antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SNAI1.

View Alternative Names

SNAH, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SNAI1 antibody [HL2303] (AB317193)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SNAI1 antibody [HL2303] (AB317193)

ab317193 detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample : Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : SNAI1 stained by ab317193 diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

Western blot - Anti-SNAI1 antibody [HL2303] (AB317193)
  • WB

Supplier Data

Western blot - Anti-SNAI1 antibody [HL2303] (AB317193)

Untreated (–) and treated (+) HeLa whole cell extract (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317193 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SNAI1 antibody [HL2303] (ab317193) at 1/1000 dilution

Lane 1:

Untreated (–) HeLa whole cell extract at 30 µg

Lane 2:

1 ng/mL TGF-beta and 300 ng/mL EGF for 2 hrs treated (+) HeLa whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-SNAI1 antibody [HL2303] (AB317193)
  • WB

Supplier Data

Western blot - Anti-SNAI1 antibody [HL2303] (AB317193)

Wild-type (WT) and SNAI1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317193 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-SNAI1 antibody [HL2303] (ab317193) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

SNAI1 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2303

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SNAI1.

O95863

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAIL also known as SNAI1 is a zinc finger transcription factor involved in the regulation of cellular processes. The SNAIL protein has a mass of approximately 29 kDa and is robustly expressed in various tissues including embryonic tissues and cancerous cells. The protein functions as a repressor of transcription influencing the expression of genes associated with cellular adhesion and movement. Due to its integral role SNAIL is involved in complex regulatory networks that control cell fate and differentiation.
Biological function summary

SNAIL contributes to the epithelial-mesenchymal transition (EMT) a process critical for embryogenesis and tumor progression. It forms a part of a complex network of transcription factors that regulate cell-cell adhesion molecules like E-cadherin. SNAIL's ability to bind to specific DNA sequences allows it to suppress or activate the transcription of target genes promoting cellular metamorphosis and enabling cells to acquire motility and invade other tissues.

Pathways

SNAIL operates significantly within the TGF-beta and Wnt signaling pathways. The TGF-beta pathway enhances the expression of SNAIL which in turn represses genes that maintain the epithelial phenotype. The Wnt pathway also modulates SNAIL activity connecting it with proteins such as beta-catenin to drive EMT. These pathways highlight SNAIL's involvement in sophisticated signaling pathways that determine cell behavior adaptation and tissue remodeling.

Overexpression of SNAIL associates with cancer progression particularly in metastasis due to its role in EMT. It connects with proteins like Slug and ZEB1 in this context enhancing the invasive capabilities of cancer cells. Moreover SNAIL is implicated in fibrotic diseases where excessive tissue scarring occurs. In such conditions TGF-beta-mediated activation of SNAIL contributes to abnormal tissue remodeling and fibrosis. The involvement of SNAIL in these diseases marks it as a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration (PubMed : 10655587, PubMed : 15647282, PubMed : 20389281, PubMed : 20562920, PubMed : 21952048, PubMed : 25827072). Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed : 10655587, PubMed : 20389281, PubMed : 20562920). The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed : 20389281, PubMed : 21300290, PubMed : 23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (PubMed : 16096638). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3 (PubMed : 20121949). In addition, may also activate the CDKN2B promoter by itself (PubMed : 20121949).
See full target information SNAI1
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Product promise

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