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AB154408

Anti-SNAI3 antibody

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(1 Publication)

Rabbit Polyclonal SNAI3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SNAI3 aa 1-250.

View Alternative Names

ZNF293, SNAI3, Zinc finger protein SNAI3, Protein snail homolog 3, Zinc finger protein 293

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SNAI3 antibody (AB154408)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SNAI3 antibody (AB154408)

Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling SNAI3 using ab154408 at a 1/500 dilution.
The image in the lower panel was co-stained with Hoechst 33342.

Western blot - Anti-SNAI3 antibody (AB154408)
  • WB

Unknown

Western blot - Anti-SNAI3 antibody (AB154408)

12% SDS PAGE

All lanes:

Western blot - Anti-SNAI3 antibody (ab154408) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SNAI3 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q3KNW1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAI3 also known as Snail family transcriptional repressor 3 is a zinc-finger protein involved in transcription regulation. It has a molecular mass of approximately 30-34 kDa. SNAI3 is expressed in various tissues but shows significant expression in mesodermal and neuroectodermal cells. The protein functions by binding to E-box motifs in the promoter regions of target genes and repressing their transcription.
Biological function summary

SNAI3 plays a role in cellular processes including epithelial-mesenchymal transition (EMT) which is critical for development and wound healing. SNAI3 does not form part of a large protein complex but interacts with other molecules to exert its regulatory function. Its activity helps facilitate changes in cell adhesion and motility impacting developmental pathways and cellular reprogramming.

Pathways

SNAI3 plays roles in EMT and the Wnt signaling pathway. The Wnt pathway is important in cell proliferation and determination and SNAI3 is known to interact with key proteins such as β-catenin to modulate these processes. SNAI3 regulates genes involved in these pathways integrating signals that drive changes in cell state and phenotype during morphogenesis.

SNAI3's function connects to cancer and fibrosis. In cancer deregulated SNAI3 expression can promote metastasis by facilitating EMT reminiscent of its role in normal tissue maintenance. Proteins like Twist1 and ZEB1 interact with SNAI3 in these contexts possibly contributing to aggressive cancer phenotypes. In fibrosis altered SNAI3 expression affects tissue remodeling partially due to its influence on extracellular matrix composition.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Seems to inhibit myoblast differentiation. Transcriptional repressor of E-box-dependent transactivation of downstream myogenic bHLHs genes. Binds preferentially to the canonical E-box sequences 5'-CAGGTG-3' and 5'-CACCTG-3' (By similarity).
See full target information SNAI3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 33:145-159.e6 PubMed33357456

2020

High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sangmoo Jeong,Angela Maria Savino,Rachel Chirayil,Ersilia Barin,Yuanming Cheng,Sun-Mi Park,Alexandra Schurer,Edouard Mullarky,Lewis C Cantley,Michael G Kharas,Kayvan R Keshari
View all publications
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Product promise

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