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Knockout Tested Rabbit Recombinant Monoclonal SNAIL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 21 publications.

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Images

Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (AB229701), expandable thumbnail
  • Immunoprecipitation - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (AB229701), expandable thumbnail
  • Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (AB229701), expandable thumbnail
  • Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (AB229701), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration (PubMed:10655587, PubMed:15647282, PubMed:20389281, PubMed:20562920, PubMed:21952048, PubMed:25827072). Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed:10655587, PubMed:20389281, PubMed:20562920). The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed:20389281, PubMed:21300290, PubMed:23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (PubMed:16096638). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3 (PubMed:20121949). In addition, may also activate the CDKN2B promoter by itself (PubMed:20121949).

Alternative names

SNAH, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal SNAIL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 21 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR21043
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab229701 is the carrier-free version of Anti-SNAIL antibody [EPR21043] ab216347.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SNAIL also known as SNAI1 is a zinc finger transcription factor involved in the regulation of cellular processes. The SNAIL protein has a mass of approximately 29 kDa and is robustly expressed in various tissues including embryonic tissues and cancerous cells. The protein functions as a repressor of transcription influencing the expression of genes associated with cellular adhesion and movement. Due to its integral role SNAIL is involved in complex regulatory networks that control cell fate and differentiation.

Biological function summary

SNAIL contributes to the epithelial-mesenchymal transition (EMT) a process critical for embryogenesis and tumor progression. It forms a part of a complex network of transcription factors that regulate cell-cell adhesion molecules like E-cadherin. SNAIL's ability to bind to specific DNA sequences allows it to suppress or activate the transcription of target genes promoting cellular metamorphosis and enabling cells to acquire motility and invade other tissues.

Pathways

SNAIL operates significantly within the TGF-beta and Wnt signaling pathways. The TGF-beta pathway enhances the expression of SNAIL which in turn represses genes that maintain the epithelial phenotype. The Wnt pathway also modulates SNAIL activity connecting it with proteins such as beta-catenin to drive EMT. These pathways highlight SNAIL's involvement in sophisticated signaling pathways that determine cell behavior adaptation and tissue remodeling.

Associated diseases and disorders

Overexpression of SNAIL associates with cancer progression particularly in metastasis due to its role in EMT. It connects with proteins like Slug and ZEB1 in this context enhancing the invasive capabilities of cancer cells. Moreover SNAIL is implicated in fibrotic diseases where excessive tissue scarring occurs. In such conditions TGF-beta-mediated activation of SNAIL contributes to abnormal tissue remodeling and fibrosis. The involvement of SNAIL in these diseases marks it as a potential target for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (ab229701), expandable thumbnail

    Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (ab229701)

    False colour image of Western blot: Anti-SNAIL antibody [EPR21043] staining at 1/500 dilution, shown in green; loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, Anti-SNAIL antibody [EPR21043] ab216347 was shown to bind specifically to SNAIL. A band was observed at 33 kDa in wild-type HeLa cell lysates with no signal observed at this size in SNAI1 CRISPR-Cas9 edited cell line Human SNAI1 (SNAIL) knockout HeLa cell line ab265963 (CRISPR-Cas9 edited cell lysate Human SNAI1 (SNAIL) knockout HeLa cell lysate ab257692). The band observed in the CRISPR-Cas9 edited lysate lane below 33 kDa is likely to represent a truncated form of SNAIL. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and SNAI1 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-SNAIL antibody [EPR21043] (Anti-SNAIL antibody [EPR21043] ab216347) at 1/500 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: SNAI1 CRISPR-Cas9 edited HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human SNAI1 (SNAIL) knockout HeLa cell line (Human SNAI1 (SNAIL) knockout HeLa cell line ab265963)

    Performed under reducing conditions.

    Predicted band size: 29 kDa

    Observed band size: 33 kDa

  • Immunoprecipitation - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (ab229701), expandable thumbnail

    Immunoprecipitation - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (ab229701)

    SNAIL was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-SNAIL antibody [EPR21043] ab216347 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-SNAIL antibody [EPR21043] ab216347 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).
    Lane 2: Anti-SNAIL antibody [EPR21043] ab216347 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SNAIL antibody [EPR21043] ab216347 in HeLa whole cell lysate.

    Exposure time: 10 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SNAIL antibody [EPR21043] ab216347).

    All lanes: Immunoprecipitation - Anti-SNAIL antibody [EPR21043] (Anti-SNAIL antibody [EPR21043] ab216347)

    Developed using the ECL technique.

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

  • Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (ab229701), expandable thumbnail

    Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (ab229701)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    SNAIL expression is not detectable in MCF7 cells, which is consistent with what has been described in the literature (PMID: 10655587 and 22028892).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SNAIL antibody [EPR21043] ab216347).

    All lanes: Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (ab229701) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

    Exposure time: 3min

  • Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (ab229701), expandable thumbnail

    Western blot - Anti-SNAIL antibody [EPR21043] - BSA and Azide free (ab229701)

    This data was developed using the same antibody clone in a different buffer formulation (abAB216347).

    Western blot: Anti-SNAI1 antibody [EPR21043] (Anti-SNAIL antibody [EPR21043] ab216347) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-SNAIL antibody [EPR21043] ab216347 was shown to bind specifically to SNAI1. A band was observed at 29 kDa in wild-type A549 cell lysates with no signal observed at this size in SNAI1 knockout cell line. To generate this image, wild-type and SNAI1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-SNAIL antibody [EPR21043] (Anti-SNAIL antibody [EPR21043] ab216347) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: SNAI1 knockout A549 cell lysate at 20 µg

    Lane 3: Wild-type HeLa ab255929 cell lysate at 20 µg

    Lane 4: SNAI1 knockout HeLa Anti-SNAIL antibody [EPR21043] ab216347 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 29 kDa

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