Mouse Polyclonal SNAIL antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNAI1.
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- Cells 12:2023Lymphatic Endothelial-to-Myofibroblast Transition: A Potential New Mechanism Underlying Skin Fibrosis in Systemic Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Rosa,Eloisa Romano,Bianca Saveria Fioretto,Khadija El Aoufy,Silvia Bellando-Randone,Marco Matucci-Cerinic,Mirko ManettiPubMed 37681927
- Biomolecules & therapeutics 30:340-3472022PRR16/Largen Induces Epithelial-Mesenchymal Transition through the Interaction with ABI2 Leading to the Activation of ABL1 Kinase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGyeoung Jin Kang,Jung Ho Park,Hyun Ji Kim,Eun Ji Kim,Boram Kim,Hyun Jung Byun,Lu Yu,Tuan Minh Nguyen,Thi Ha Nguyen,Kyung Sung Kim,Hiệu Phùng Huy,Mostafizur Rahman,Ye Hyeon Kim,Ji Yun Jang,Mi Kyung Park,Ho Lee,Chang Ick Choi,Kyeong Lee,Hyo Kyung Han,Jungsook Cho,Seung Bae Rho,Chang Hoon LeePubMed 35719027
- Bioengineered 13:4744-47562022Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Dang,Cuiping Shi,Qianqian Zhang,Peiyu Liao,Yan WangPubMed 35138218
- International journal of oncology 58:2021Reduced kinase D‑interacting substrate of 220 kDa (Kidins220) in pancreatic cancer promotes EGFR/ERK signalling and disease progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Cai,Zhiwei Sun,Ping-Hui Sun,Xiangyu Gao,Ke Ji,Xiuyun Tian,Jiafu Ji,Chunyi Hao,Faris Soliman,Chang Liu,Bilal Al-Sarireh,Paul Griffiths,Stephen Hiscox,Wen G Jiang,Lin YePubMed 33955519
- Journal of cellular and molecular medicine 24:9658-96662020Oestrogen induces epithelial-mesenchymal transition in endometriosis via circ_0004712/miR-148a-3p sponge function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin He et. al.PubMed 32667746
- Journal of cellular and molecular medicine 23:8035-80452019E -mediated EMT by activation of β-catenin/Snail signalling during the development of ovarian endometriosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenqian Xiong et. al.PubMed 31560827
- Oncology letters 17:1189-11952019MicroRNA-153 suppresses cell invasion by targeting SNAI1 and predicts patient prognosis in glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhao,Chang-You Yin,Jing Jiang,Wei Kong,Hao Xu,Hongtao ZhangPubMed 30655883
- Bioscience reports 38:2018Tamoxifen attenuates dialysate-induced peritoneal fibrosis by inhibiting GSK-3β/β-catenin axis activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengpeng Yan et. al.PubMed 30061174
- Journal of experimental & clinical cancer research 37:2312018HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Li et. al.PubMed 30231922
- Scientific reports 8:105942018Fibulin-3 knockdown inhibits cervical cancer cell growth and metastasis in vitro and in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Li et. al.PubMed 30006571
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human SNAI1. Database link O95863
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Transfected cell lysate | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Associated Products
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Target data
Function
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration (PubMed:10655587, PubMed:15647282, PubMed:20389281, PubMed:20562920, PubMed:21952048, PubMed:25827072). Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed:10655587, PubMed:20389281, PubMed:20562920). The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed:20389281, PubMed:21300290, PubMed:23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (PubMed:16096638). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3 (PubMed:20121949). In addition, may also activate the CDKN2B promoter by itself (PubMed:20121949).
Additional Targets
SNAI2
Alternative names
SNAH, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna
Recommended products
Mouse Polyclonal SNAIL antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNAI1.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human SNAI1. Database link O95863
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAIL and SLUG also known as Snai1 and Snai2 are zinc finger transcription factors important in the regulation of epithelial-mesenchymal transition (EMT). These proteins are expressed mainly in various developing tissues and tumors. The molecular weight of SNAIL is approximately 29 kDa while SLUG typically has a mass around 30 kDa. These factors function within the nucleus binding to E-box sequences in DNA to repress the transcription of epithelial markers.
- Biological function summary
SNAIL and SLUG operate as vital regulators of cell motility and invasion by modulating gene expression. They do not work alone; they participate as components of a complex with other EMT-inducing transcription factors including TWIST and ZEB. These factors suppress E-cadherin and other adhesion molecules which facilitates the disassembly of tight junctions between cells promoting a mesenchymal phenotype that is more migratory and invasive.
- Pathways
These transcription factors play key roles in both the TGF-beta and Wnt signaling pathways. In the TGF-beta pathway SNAIL and SLUG regulate gene expression to support EMT affecting processes in development and cancer progression. Within the Wnt pathway these factors interact with beta-catenin impacting cell cycle regulation and supporting cells' transition between epithelial and mesenchymal states a critical step in tumor metastasis.
- Associated diseases and disorders
SNAIL and SLUG significantly relate to cancer and fibrosis. In cancer their interaction with proteins such as ZEB and TWIST is important for the metastasis of carcinoma cells. In fibrosis these transcription factors link to TGF-beta-induced fibroblast activation contributing to tissue scarring. The dysregulation of SNAIL and SLUG often serves as an indicator of poor prognosis due to their roles in promoting invasive and metastatic properties in cancer.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-SNAIL + SLUG antibody (ab167609)
All lanes: Western blot - Anti-SNAIL + SLUG antibody (ab167609) at 1 µg/mL
Lane 1: SNAIL transfected 293T cell lysate at 15 µL
Lane 2: Non-transfected 293T cell lysate at 15 µL
SecondaryAll lanes: Goat anti-Mouse IgG
Predicted band size: 29 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody (ab167609)
Immunofluorescent analysis of HeLa cells labeling SNAIL with ab167609 at 10 μg/ml.
Western blot - Anti-SNAIL + SLUG antibody (ab167609)
Substrate: Perkin Elmer
Lane 1: Monoclonal Mouse anti-GST at 1 µg
Lanes 2 and 5: Milk at 5 %
Lane 3: Western blot - Anti-SNAIL + SLUG antibody (ab167609) at 1 µg
Lane 4: Monoclonal Mouse anti-GST at 1 µg/mL
Lane 6: Western blot - Anti-SNAIL + SLUG antibody (ab167609) at 1 µg/mL
Lanes 1 - 3: SNAIL-GST fusion protein at 0.2 µg
Lanes 4 - 6: SLUG-GST-fusion protein at 0.2 µg
SecondaryAll lanes: HRP conjugated Goat anti-mouse at 1/5000 dilution
Predicted band size: 29 kDa
Observed band size: 55.5 kDa, 56.4 kDa
Exposure time: 90s
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