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AB167609

Anti-SNAIL + SLUG antibody

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(22 Publications)

Mouse Polyclonal SNAIL antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Human samples. Cited in 22 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNAI1.

View Alternative Names

SNAH, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna, SLUG, SLUGH, SNAI2, Zinc finger protein SNAI2, Neural crest transcription factor Slug, Protein snail homolog 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody (AB167609)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody (AB167609)

Immunofluorescent analysis of HeLa cells labeling SNAIL with ab167609 at 10 μg/ml.

Western blot - Anti-SNAIL + SLUG antibody (AB167609)
  • WB

Unknown

Western blot - Anti-SNAIL + SLUG antibody (AB167609)

Substrate : Perkin Elmer

Lane 1:

Monoclonal Mouse anti-GST at 1 µg

Lanes 2 and 5:

Milk at 5 %

Lane 3:

Western blot - Anti-SNAIL + SLUG antibody (ab167609) at 1 µg

Lane 4:

Monoclonal Mouse anti-GST at 1 µg/mL

Lane 6:

Western blot - Anti-SNAIL + SLUG antibody (ab167609) at 1 µg/mL

Lanes 1 - 3:

SNAIL-GST fusion protein at 0.2 µg

Lanes 4 - 6:

SLUG-GST-fusion protein at 0.2 µg

Secondary

All lanes:

HRP conjugated Goat anti-mouse at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 55.5 kDa,56.4 kDa

false

Exposure time: 90s

Western blot - Anti-SNAIL + SLUG antibody (AB167609)
  • WB

Unknown

Western blot - Anti-SNAIL + SLUG antibody (AB167609)

All lanes:

Western blot - Anti-SNAIL + SLUG antibody (ab167609) at 1 µg/mL

Lane 1:

SNAIL transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat anti-Mouse IgG

Predicted band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SNAI1.

O95863

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAIL and SLUG also known as Snai1 and Snai2 are zinc finger transcription factors important in the regulation of epithelial-mesenchymal transition (EMT). These proteins are expressed mainly in various developing tissues and tumors. The molecular weight of SNAIL is approximately 29 kDa while SLUG typically has a mass around 30 kDa. These factors function within the nucleus binding to E-box sequences in DNA to repress the transcription of epithelial markers.
Biological function summary

SNAIL and SLUG operate as vital regulators of cell motility and invasion by modulating gene expression. They do not work alone; they participate as components of a complex with other EMT-inducing transcription factors including TWIST and ZEB. These factors suppress E-cadherin and other adhesion molecules which facilitates the disassembly of tight junctions between cells promoting a mesenchymal phenotype that is more migratory and invasive.

Pathways

These transcription factors play key roles in both the TGF-beta and Wnt signaling pathways. In the TGF-beta pathway SNAIL and SLUG regulate gene expression to support EMT affecting processes in development and cancer progression. Within the Wnt pathway these factors interact with beta-catenin impacting cell cycle regulation and supporting cells' transition between epithelial and mesenchymal states a critical step in tumor metastasis.

SNAIL and SLUG significantly relate to cancer and fibrosis. In cancer their interaction with proteins such as ZEB and TWIST is important for the metastasis of carcinoma cells. In fibrosis these transcription factors link to TGF-beta-induced fibroblast activation contributing to tissue scarring. The dysregulation of SNAIL and SLUG often serves as an indicator of poor prognosis due to their roles in promoting invasive and metastatic properties in cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration (PubMed : 10655587, PubMed : 15647282, PubMed : 20389281, PubMed : 20562920, PubMed : 21952048, PubMed : 25827072). Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed : 10655587, PubMed : 20389281, PubMed : 20562920). The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed : 20389281, PubMed : 21300290, PubMed : 23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (PubMed : 16096638). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3 (PubMed : 20121949). In addition, may also activate the CDKN2B promoter by itself (PubMed : 20121949).
See full target information SNAI1

Additional targets

SNAI2
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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 43:2708-2721 PubMed39112518

2024

CRISPR-based dissection of microRNA-23a ~ 27a ~ 24-2 cluster functionality in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengying Cui,Zhichao Liu,Shuaibin Wang,Sejong Bae,Hua Guo,Jiangbing Zhou,Runhua Liu,Lizhong Wang

Cells 12: PubMed37681927

2023

Lymphatic Endothelial-to-Myofibroblast Transition: A Potential New Mechanism Underlying Skin Fibrosis in Systemic Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Rosa,Eloisa Romano,Bianca Saveria Fioretto,Khadija El Aoufy,Silvia Bellando-Randone,Marco Matucci-Cerinic,Mirko Manetti

Biomolecules & therapeutics 30:340-347 PubMed35719027

2022

PRR16/Largen Induces Epithelial-Mesenchymal Transition through the Interaction with ABI2 Leading to the Activation of ABL1 Kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Gyeoung Jin Kang,Jung Ho Park,Hyun Ji Kim,Eun Ji Kim,Boram Kim,Hyun Jung Byun,Lu Yu,Tuan Minh Nguyen,Thi Ha Nguyen,Kyung Sung Kim,Hiệu Phùng Huy,Mostafizur Rahman,Ye Hyeon Kim,Ji Yun Jang,Mi Kyung Park,Ho Lee,Chang Ick Choi,Kyeong Lee,Hyo Kyung Han,Jungsook Cho,Seung Bae Rho,Chang Hoon Lee

Bioengineered 13:4744-4756 PubMed35138218

2022

Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Dang,Cuiping Shi,Qianqian Zhang,Peiyu Liao,Yan Wang

International journal of oncology 58: PubMed33955519

2021

Reduced kinase D‑interacting substrate of 220 kDa (Kidins220) in pancreatic cancer promotes EGFR/ERK signalling and disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Cai,Zhiwei Sun,Ping-Hui Sun,Xiangyu Gao,Ke Ji,Xiuyun Tian,Jiafu Ji,Chunyi Hao,Faris Soliman,Chang Liu,Bilal Al-Sarireh,Paul Griffiths,Stephen Hiscox,Wen G Jiang,Lin Ye

Journal of cellular and molecular medicine 24:9658-9666 PubMed32667746

2020

Oestrogen induces epithelial-mesenchymal transition in endometriosis via circ_0004712/miR-148a-3p sponge function.

Applications

Unspecified application

Species

Unspecified reactive species

Xin He,Nana Liu,Tianyi Mu,Dan Lu,Chanwei Jia,Shuyu Wang,Chenghong Yin,Lingyan Liu,Liying Zhou,Xiaowu Huang,Yanmin Ma

Journal of cellular and molecular medicine 23:8035-8045 PubMed31560827

2019

E -mediated EMT by activation of β-catenin/Snail signalling during the development of ovarian endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Xiong,Ling Zhang,Hengwei Liu,Na Li,Yu Du,Haitang He,Zhibing Zhang,Yi Liu

Oncology letters 17:1189-1195 PubMed30655883

2019

MicroRNA-153 suppresses cell invasion by targeting SNAI1 and predicts patient prognosis in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhao,Chang-You Yin,Jing Jiang,Wei Kong,Hao Xu,Hongtao Zhang

Bioscience reports 38: PubMed30061174

2018

Tamoxifen attenuates dialysate-induced peritoneal fibrosis by inhibiting GSK-3β/β-catenin axis activation.

Applications

Unspecified application

Species

Unspecified reactive species

Pengpeng Yan,Huanna Tang,Xiaoying Chen,Shuiyu Ji,Wei Jin,Jiaming Zhang,Jia Shen,Hao Deng,Xiang Zhao,Quanquan Shen,Hongfeng Huang

Journal of experimental & clinical cancer research 37:231 PubMed30231922

2018

HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Cong Wang,Yufeng Wang,Liankang Sun,Zhikui Liu,Liang Wang,Tao Song,Yingmin Yao,Qingguang Liu,Kangsheng Tu
View all publications
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