Anti-SNAIL + SLUG antibody

• WB

Supplier Data

Western blot - Anti-SNAIL + SLUG antibody (AB180714)

All lanes:

Western blot - Anti-SNAIL + SLUG antibody (ab180714) at 1/500 dilution

All lanes:

Mouse heart tissue extracts

Predicted band size: 29 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (AB180714)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SNAIL +SLUG with ab180714 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (AB180714)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification : 400X.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (AB180714)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification : 200X.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (AB180714)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling SNAIL + SLUG with ab180714 at 1/100 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (AB180714)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SNAI1+SLUG with ab180714 at 1/50 dilution.

• IHC-P

AbReview51119****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (AB180714)

ab180714 staining SNAIL in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a basic buffer (TRIS+EDTA, pH9). Samples were incubated with primary antibody (1/500 in antibody diluent) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

Image is courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-SNAIL + SLUG antibody (AB180714)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-SNAIL + SLUG antibody (ab180714) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate at 25 µg

Lane 2:

293T whole cell lysate at 25 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 29 kDa

false

Exposure time: 90s

• WB

Supplier Data

Western blot - Anti-SNAIL + SLUG antibody (AB180714)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-SNAIL + SLUG antibody (ab180714) at 1/1000 dilution

All lanes:

Mouse kidney at 20 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 29 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-SNAIL + SLUG antibody (AB180714)

Lane 1 : Snail recombinant 0.1 ug (55 kDa) Lane 2 : Slug recombinant 0.1 ug (56 kDa)

All lanes:

Snail recombinant 0.1 ug

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-SNAIL + SLUG antibody (AB180714)

All lanes:

Western blot - Anti-SNAIL + SLUG antibody (ab180714)

Lane 1:

HeLa cell lysate

Lane 2:

A431 cell lysate

Lane 3:

HepG2 cell lysate

Lane 4:

Mouse lung tissue lysate

Lane 5:

Rat liver tissue lysate

Predicted band size: 29 kDa

false