Mouse Monoclonal SNAIL antibody. N-terminal. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNAI1 aa 1-50.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunocytochemistry-immunofluorescence-img56.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94816.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132082.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24012.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42664.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (AB224731)")
Publications
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- Nature cell biology 24:896-9052022Mechanical force application to the nucleus regulates nucleocytoplasmic transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIon Andreu,Ignasi Granero-Moya,Nimesh R Chahare,Kessem Clein,Marc Molina-Jordán,Amy E M Beedle,Alberto Elosegui-Artola,Juan F Abenza,Leone Rossetti,Xavier Trepat,Barak Raveh,Pere Roca-CusachsPubMed 35681009
- Nanoscale 14:2617-26272022Revealing the heterogeneity in neuroblastoma cells nanopillar-guided subnuclear deformation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongpeng Zeng,Priya Dharshana Ramani,Weibo Gao,Wenting ZhaoPubMed 35133394
- Cell death and differentiation 29:861-8732022O-GlcNAcylation of MORC2 at threonine 556 by OGT couples TGF-β signaling to breast cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Ying Liu,Hong-Yi Liu,Tian-Jian Yu,Qin Lu,Fang-Lin Zhang,Guang-Yu Liu,Zhi-Ming Shao,Da-Qiang LiPubMed 34974534
- Journal of translational medicine 19:2102021CCL21 activation of the MALAT1/SRSF1/mTOR axis underpins the development of gastric carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianmei Fu,Xiaohong Tan,Huaming Tang,Jijiang LiuPubMed 34001131
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human SNAI1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O95863
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/10000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration (PubMed:10655587, PubMed:15647282, PubMed:20389281, PubMed:20562920, PubMed:21952048, PubMed:25827072). Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed:10655587, PubMed:20389281, PubMed:20562920). The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed:20389281, PubMed:21300290, PubMed:23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (PubMed:16096638). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3 (PubMed:20121949). In addition, may also activate the CDKN2B promoter by itself (PubMed:20121949).
Additional Targets
SNAI2
Alternative names
SNAH, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna
Recommended products
Mouse Monoclonal SNAIL antibody. N-terminal. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNAI1 aa 1-50.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human SNAI1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O95863
- Clone number
- CL3700
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAIL and SLUG also known as Snai1 and Snai2 are zinc finger transcription factors important in the regulation of epithelial-mesenchymal transition (EMT). These proteins are expressed mainly in various developing tissues and tumors. The molecular weight of SNAIL is approximately 29 kDa while SLUG typically has a mass around 30 kDa. These factors function within the nucleus binding to E-box sequences in DNA to repress the transcription of epithelial markers.
- Biological function summary
SNAIL and SLUG operate as vital regulators of cell motility and invasion by modulating gene expression. They do not work alone; they participate as components of a complex with other EMT-inducing transcription factors including TWIST and ZEB. These factors suppress E-cadherin and other adhesion molecules which facilitates the disassembly of tight junctions between cells promoting a mesenchymal phenotype that is more migratory and invasive.
- Pathways
These transcription factors play key roles in both the TGF-beta and Wnt signaling pathways. In the TGF-beta pathway SNAIL and SLUG regulate gene expression to support EMT affecting processes in development and cancer progression. Within the Wnt pathway these factors interact with beta-catenin impacting cell cycle regulation and supporting cells' transition between epithelial and mesenchymal states a critical step in tumor metastasis.
- Associated diseases and disorders
SNAIL and SLUG significantly relate to cancer and fibrosis. In cancer their interaction with proteins such as ZEB and TWIST is important for the metastasis of carcinoma cells. In fibrosis these transcription factors link to TGF-beta-induced fibroblast activation contributing to tissue scarring. The dysregulation of SNAIL and SLUG often serves as an indicator of poor prognosis due to their roles in promoting invasive and metastatic properties in cancer.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunocytochemistry-immunofluorescence-img56.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)
PFA-fixed, Triton X-100 permeabilized BeWo cells stained for SNAI1 (Green) using ab224731 at 4 μg/ml in ICC/IF.
Microtublues are stained red.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94816.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)
Paraffin-embedded human colorectal cancer tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132082.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)
Paraffin-embedded human breast cancer tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24012.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)
Paraffin-embedded human fallopian tube tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42664.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)
Paraffin-embedded human colon tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91716.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)
Paraffin-embedded human testis tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127118.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)
Paraffin-embedded human cortex tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img50317.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)
Paraffin-embedded human prostate tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91716.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127118.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail](https://content.abcam.com/products/images/snail-slug-antibody-cl3700-n-terminal-ab224731--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img50317.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)")
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