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Mouse Monoclonal SNAIL antibody. N-terminal. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNAI1 aa 1-50.

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Images

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human SNAI1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O95863
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested
Mouse
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/5000.00000 - 1/10000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2.00000-10.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed:20389281, PubMed:20562920). The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed:20389281, PubMed:21300290, PubMed:23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.

Alternative names

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Mouse Monoclonal SNAIL antibody. N-terminal. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNAI1 aa 1-50.

Alternative names

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human SNAI1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O95863
Clone number

CL3700

Purification technique

Affinity purification Protein A

Concentration
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Purification notes

Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

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Supplementary info

Biological function summary

SNAIL and SLUG operate as vital regulators of cell motility and invasion by modulating gene expression. They do not work alone; they participate as components of a complex with other EMT-inducing transcription factors including TWIST and ZEB. These factors suppress E-cadherin and other adhesion molecules which facilitates the disassembly of tight junctions between cells promoting a mesenchymal phenotype that is more migratory and invasive.

Activity summary

SNAIL and SLUG also known as Snai1 and Snai2 are zinc finger transcription factors important in the regulation of epithelial-mesenchymal transition (EMT). These proteins are expressed mainly in various developing tissues and tumors. The molecular weight of SNAIL is approximately 29 kDa while SLUG typically has a mass around 30 kDa. These factors function within the nucleus binding to E-box sequences in DNA to repress the transcription of epithelial markers.

Pathways

These transcription factors play key roles in both the TGF-beta and Wnt signaling pathways. In the TGF-beta pathway SNAIL and SLUG regulate gene expression to support EMT affecting processes in development and cancer progression. Within the Wnt pathway these factors interact with beta-catenin impacting cell cycle regulation and supporting cells' transition between epithelial and mesenchymal states a critical step in tumor metastasis.

Associated diseases and disorders

SNAIL and SLUG significantly relate to cancer and fibrosis. In cancer their interaction with proteins such as ZEB and TWIST is important for the metastasis of carcinoma cells. In fibrosis these transcription factors link to TGF-beta-induced fibroblast activation contributing to tissue scarring. The dysregulation of SNAIL and SLUG often serves as an indicator of poor prognosis due to their roles in promoting invasive and metastatic properties in cancer.

Product promise

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Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)

    PFA-fixed, Triton X-100 permeabilized BeWo cells stained for SNAI1 (Green) using ab224731 at 4 μg/ml in ICC/IF.

    Microtublues are stained red.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)

    Paraffin-embedded human colorectal cancer tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)

    Paraffin-embedded human breast cancer tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)

    Paraffin-embedded human fallopian tube tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)

    Paraffin-embedded human colon tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)

    Paraffin-embedded human testis tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)

    Paraffin-embedded human cortex tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody [CL3700] - N-terminal (ab224731)

    Paraffin-embedded human prostate tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

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Product protocols

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