Rabbit Polyclonal SNAIL phospho S246 antibody. Suitable for WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human SNAI1 phospho S246.
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- Aging 13:15523-155372021S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhigang Xu,Chuantao Cheng,Ranran Kong,Yale Liu,Shuang Wang,Yuefeng Ma,Xin XingPubMed 34099591
- Cell biology international 45:1288-12952021Dual inhibition of TGFβR and ROCK reverses the epithelial to mesenchymal transition in collectively migrating zebrafish keratocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnes S Pascual,Jose L Rapanan,Chandana K Uppalapati,Kimbal E Cooper,Kathryn J Leyva,Elizabeth E HullPubMed 33710707
- Journal of cellular physiology 235:8768-87782020A Slug-dependent mechanism is responsible for tumor suppression of p53-stabilizing compound CP-31398 in p53-mutated endometrial carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Liu,Zhi-Ying Yu,Tan-Tan Yu,Shi-Hong Cui,Li Yang,Hui Chang,Yan-Hong Qu,Xiao-Feng Lv,Xiao-An Zhang,Chen-Chen RenPubMed 32633026
- Journal of experimental & clinical cancer research 37:2372018Long noncoding RNA MLK7-AS1 promotes ovarian cancer cells progression by modulating miR-375/YAP1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Yan et. al.PubMed 30249278
- Nature communications 9:34732018Group I Paks are essential for epithelial- mesenchymal transition in an Apc-driven model of colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH Y Chow et. al.PubMed 30150766
- Oncotarget 8:37717-377292017KCTD11 inhibits growth and metastasis of hepatocellular carcinoma through activating Hippo signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongliang Tong,Beng Yang,Heng Xiao,Chuanhui Peng,Wendi Hu,Xiaoyu Weng,Shaobing Cheng,Chengli Du,Zhen Lv,Chaofeng Ding,Lin Zhou,Haiyang Xie,Jian Wu,Shusen ZhengPubMed 28465479
- PloS one 6:e290602011Regulation of cysteinyl leukotriene receptor 2 expression--a potential anti-tumor mechanism.Applications:WBReactive species:HumanCecilia Magnusson,Astrid M Bengtsson,Minghui Liu,Jian Liu,Yvonne Ceder,Roy Ehrnström,Anita SjölanderPubMed 22194989
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SNAI1 phospho S246. The exact immunogen used to generate this antibody is proprietary information. Database link O95863
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Recombinant full length protein - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration (PubMed:10655587, PubMed:15647282, PubMed:20389281, PubMed:20562920, PubMed:21952048, PubMed:25827072). Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed:10655587, PubMed:20389281, PubMed:20562920). The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed:20389281, PubMed:21300290, PubMed:23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (PubMed:16096638). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3 (PubMed:20121949). In addition, may also activate the CDKN2B promoter by itself (PubMed:20121949).
Alternative names
SNAH, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna
Recommended products
Rabbit Polyclonal SNAIL phospho S246 antibody. Suitable for WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human SNAI1 phospho S246.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SNAI1 phospho S246. The exact immunogen used to generate this antibody is proprietary information. Database link O95863
- Purification technique
- Affinity purification Immunogen
- Specificity
ab63568 detects endogenous levels of SNAIL only when phosphorylated at serine 246.
- Concentration
- Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAIL and SLUG also known as Snai1 and Snai2 are zinc finger transcription factors important in the regulation of epithelial-mesenchymal transition (EMT). These proteins are expressed mainly in various developing tissues and tumors. The molecular weight of SNAIL is approximately 29 kDa while SLUG typically has a mass around 30 kDa. These factors function within the nucleus binding to E-box sequences in DNA to repress the transcription of epithelial markers.
- Biological function summary
SNAIL and SLUG operate as vital regulators of cell motility and invasion by modulating gene expression. They do not work alone; they participate as components of a complex with other EMT-inducing transcription factors including TWIST and ZEB. These factors suppress E-cadherin and other adhesion molecules which facilitates the disassembly of tight junctions between cells promoting a mesenchymal phenotype that is more migratory and invasive.
- Pathways
These transcription factors play key roles in both the TGF-beta and Wnt signaling pathways. In the TGF-beta pathway SNAIL and SLUG regulate gene expression to support EMT affecting processes in development and cancer progression. Within the Wnt pathway these factors interact with beta-catenin impacting cell cycle regulation and supporting cells' transition between epithelial and mesenchymal states a critical step in tumor metastasis.
- Associated diseases and disorders
SNAIL and SLUG significantly relate to cancer and fibrosis. In cancer their interaction with proteins such as ZEB and TWIST is important for the metastasis of carcinoma cells. In fibrosis these transcription factors link to TGF-beta-induced fibroblast activation contributing to tissue scarring. The dysregulation of SNAIL and SLUG often serves as an indicator of poor prognosis due to their roles in promoting invasive and metastatic properties in cancer.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
All lanes: Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1: Extracts from HT29 cells (5-30µg), minus immunising peptide
Lane 2: Extracts from HT29 cells (5-30µg) plus immunising peptide (5-10µg)
Predicted band size: 29 kDa
Observed band size: 26 kDa
Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
ab63568 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa
All lanes: Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1: SNAIL (SNAI1) Human Recombinant Protein at 0.1 µg
Lane 2: SLUG (SNAI2) Human Recombinant Protein at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa
Exposure time: 3min
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