AB63568

Anti-SNAIL + SLUG (phospho S246) antibody

Name: Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) | Abcam
Brand: Abcam Limited
SKU: AB63568
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal SNAIL phospho S246 antibody. Suitable for WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SNAI1 phospho S246.

View Alternative Names

SNAH, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna

2 Images

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Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (AB63568)

All lanes:

Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution

Lane 1:

Extracts from HT29 cells (5-30µg), minus immunising peptide

Lane 2:

Extracts from HT29 cells (5-30µg) plus immunising peptide (5-10µg)

Predicted band size: 29 kDa

Observed band size: 26 kDa

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Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (AB63568)

ab63568 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa

All lanes:

Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution

Lane 1:

SNAIL (SNAI1) Human Recombinant Protein at 0.1 µg

Lane 2:

SLUG (SNAI2) Human Recombinant Protein at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 68 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human SNAI1 phospho S246. The exact immunogen used to generate this antibody is proprietary information.

O95863

Specificity

ab63568 detects endogenous levels of SNAIL only when phosphorylated at serine 246.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAIL and SLUG also known as Snai1 and Snai2 are zinc finger transcription factors important in the regulation of epithelial-mesenchymal transition (EMT). These proteins are expressed mainly in various developing tissues and tumors. The molecular weight of SNAIL is approximately 29 kDa while SLUG typically has a mass around 30 kDa. These factors function within the nucleus binding to E-box sequences in DNA to repress the transcription of epithelial markers.

Biological function summary

SNAIL and SLUG operate as vital regulators of cell motility and invasion by modulating gene expression. They do not work alone; they participate as components of a complex with other EMT-inducing transcription factors including TWIST and ZEB. These factors suppress E-cadherin and other adhesion molecules which facilitates the disassembly of tight junctions between cells promoting a mesenchymal phenotype that is more migratory and invasive.

Pathways

These transcription factors play key roles in both the TGF-beta and Wnt signaling pathways. In the TGF-beta pathway SNAIL and SLUG regulate gene expression to support EMT affecting processes in development and cancer progression. Within the Wnt pathway these factors interact with beta-catenin impacting cell cycle regulation and supporting cells' transition between epithelial and mesenchymal states a critical step in tumor metastasis.

SNAIL and SLUG significantly relate to cancer and fibrosis. In cancer their interaction with proteins such as ZEB and TWIST is important for the metastasis of carcinoma cells. In fibrosis these transcription factors link to TGF-beta-induced fibroblast activation contributing to tissue scarring. The dysregulation of SNAIL and SLUG often serves as an indicator of poor prognosis due to their roles in promoting invasive and metastatic properties in cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration (PubMed : 10655587, PubMed : 15647282, PubMed : 20389281, PubMed : 20562920, PubMed : 21952048, PubMed : 25827072). Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription (PubMed : 10655587, PubMed : 20389281, PubMed : 20562920). The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (PubMed : 20389281, PubMed : 21300290, PubMed : 23721412). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (PubMed : 16096638). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3 (PubMed : 20121949). In addition, may also activate the CDKN2B promoter by itself (PubMed : 20121949).

See full target information SNAI1 phospho S246

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The clinical respiratory journal 18:e13770 PubMed38783645

2024

ZEB1-AS1 mediates bone metastasis through targeting miR-320b/BMPR1A axis in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nianxi Tan,Junyi Tang,Guang Chen,Weilin Jiang,Zhiqin Liu

Cell division 18:4 PubMed36882799

2023

MiR-146b-5p/SEMA3G regulates epithelial-mesenchymal transition in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxi Tang,Tao Xiong

Aging 13:15523-15537 PubMed34099591

2021

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn.

Applications

Unspecified application

Species

Unspecified reactive species

Zhigang Xu,Chuantao Cheng,Ranran Kong,Yale Liu,Shuang Wang,Yuefeng Ma,Xin Xing

Cell biology international 45:1288-1295 PubMed33710707

2021

Dual inhibition of TGFβR and ROCK reverses the epithelial to mesenchymal transition in collectively migrating zebrafish keratocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes S Pascual,Jose L Rapanan,Chandana K Uppalapati,Kimbal E Cooper,Kathryn J Leyva,Elizabeth E Hull

Journal of cellular physiology 235:8768-8778 PubMed32633026

2020

A Slug-dependent mechanism is responsible for tumor suppression of p53-stabilizing compound CP-31398 in p53-mutated endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Liu,Zhi-Ying Yu,Tan-Tan Yu,Shi-Hong Cui,Li Yang,Hui Chang,Yan-Hong Qu,Xiao-Feng Lv,Xiao-An Zhang,Chen-Chen Ren

Journal of experimental & clinical cancer research 37:237 PubMed30249278

2018

Long noncoding RNA MLK7-AS1 promotes ovarian cancer cells progression by modulating miR-375/YAP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Yan,Hong Li,Pengyun Li,Xia Li,Jianjian Lin,Linlin Zhu,Maria A Silva,Xiaofang Wang,Ping Wang,Zhan Zhang

Nature communications 9:3473 PubMed30150766

2018

Group I Paks are essential for epithelial- mesenchymal transition in an Apc-driven model of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

H Y Chow,B Dong,C A Valencia,C T Zeng,J N Koch,T Y Prudnikova,J Chernoff

Oncotarget 8:37717-37729 PubMed28465479

2017

KCTD11 inhibits growth and metastasis of hepatocellular carcinoma through activating Hippo signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rongliang Tong,Beng Yang,Heng Xiao,Chuanhui Peng,Wendi Hu,Xiaoyu Weng,Shaobing Cheng,Chengli Du,Zhen Lv,Chaofeng Ding,Lin Zhou,Haiyang Xie,Jian Wu,Shusen Zheng

PloS one 6:e29060 PubMed22194989

2011

Regulation of cysteinyl leukotriene receptor 2 expression--a potential anti-tumor mechanism.

Applications

Species

Human

Cecilia Magnusson,Astrid M Bengtsson,Minghui Liu,Jian Liu,Yvonne Ceder,Roy Ehrnström,Anita Sjölander

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SNAIL + SLUG (phospho S246) antibody

Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (AB63568)

Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (AB63568)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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